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中国精品科技期刊2020

克罗诺杆菌环境耐受性的评价

庞立冬 宋丹靓敏 贾嫒 李誉 梁雅琪 苏群超 郑亚平 李然 满朝新 姜毓君

庞立冬,宋丹靓敏,贾嫒,等. 克罗诺杆菌环境耐受性的评价[J]. 食品工业科技,2022,43(23):161−167. doi:  10.13386/j.issn1002-0306.2022040050
引用本文: 庞立冬,宋丹靓敏,贾嫒,等. 克罗诺杆菌环境耐受性的评价[J]. 食品工业科技,2022,43(23):161−167. doi:  10.13386/j.issn1002-0306.2022040050
PANG Lidong, SONG Danliangmin, JIA Ai, et al. Evaluation of Environmental Resistance of Cronobacter spp doi:  10.13386/j.issn1002-0306.2022040050
Citation: PANG Lidong, SONG Danliangmin, JIA Ai, et al. Evaluation of Environmental Resistance of Cronobacter spp doi:  10.13386/j.issn1002-0306.2022040050

克罗诺杆菌环境耐受性的评价

doi: 10.13386/j.issn1002-0306.2022040050
基金项目: 国家自然科学基金面上项目(31871828)。
详细信息
    作者简介:

    庞立冬(2000−),男,本科,研究方向:乳品科学技术,E-mail:325146789@qq.com

    通讯作者:

    姜毓君(1971−),男,博士,教授,研究方向:乳品科学技术,E-mail: yujun_jiang@163.com

  • 中图分类号: TS201.3

Evaluation of Environmental Resistance of Cronobacter spp.

  • 摘要: 婴儿配方奶粉(powdered infant formulas,PIF)是克罗诺杆菌(Cronobacter spp.)的主要污染来源,其原辅料的添加和加工环境是最为主要的传播途径。为彻底有效地对克罗诺杆菌进行预防和控制,保障乳制品的安全,本实验以8株分离自PIF及其加工环境中的克罗诺杆菌为研究对象,对其耐热性、耐酸碱性、耐干燥性及耐高渗性进行研究,分析不同菌株环境耐受性之间的差异。结果表明:8株克罗诺杆菌对热的耐受性存在差异性,其中分离自终产品的菌株耐热性相对较强;克罗诺杆菌普遍具有耐酸不耐碱的特性,在pH为1.5的酸性条件下,所有菌株在处理30 s后仍可存活;克罗诺杆菌具有较强的抗干燥能力,在室温条件下,空气相对湿度为20.7%的条件下,所有菌株在7个月的试验过程中菌数均从初始浓度108 CFU/mL下降到105 CFU/mL,这种缓慢的下降趋势说明克罗诺杆菌具有很强的抵抗干燥环境的能力;克罗诺杆菌具有较强的耐高渗性,当NaCl的浓度大于8%时,所有菌株生长缓慢,且在等渗条件下,克罗诺杆菌对山梨醇的耐受性强于NaCl。
  • 图  1  ES3、ES46、ES35和ES39四株克罗诺杆菌在干燥条件下的活菌数

    Figure  1.  Viable counts of four Cronobacter spp. ES3, ES46, ES35 and ES39 under dry conditions

    图  2  ES52、ES45、ES37和ES38四株克罗诺杆菌在干燥条件下的活菌数

    Figure  2.  Viable counts of four Cronobacter spp. ES52, ES45, ES37 and ES38 under dry conditions

    图  3  克罗诺杆菌对不同浓度NaCl的耐受性

    Figure  3.  Resistance of Cronobacter spp. for different concentrations of NaCl

    注:A~H分别为克罗诺杆菌ES3、ES35、ES37、ES38、ES39、ES45、ES46、ES52。

    表  1  实验用菌株及其来源

    Table  1.   Bacterial strains used in this study and their sources

    菌株编号菌株名称所属ST型菌株来源
    ES3丙二酸盐克罗诺杆菌258婴儿配方粉
    ES35丙二酸盐克罗诺杆菌201婴儿配方粉
    ES37阪崎克罗诺杆菌8原料乳
    ES38阪崎克罗诺杆菌12原辅料
    ES39阪崎克罗诺杆菌1婴儿配方粉
    ES45阪崎克罗诺杆菌4振动筛
    ES46阪崎克罗诺杆菌64传送带
    ES52阪崎克罗诺杆菌21流化床出口
    下载: 导出CSV

    表  2  克罗诺杆菌在不同温度下的生长情况

    Table  2.   Growth of Cronobacter spp. at different temperatures

    温度
    (℃)
    菌株
    编号
    时间(min)
    1235810203060
    53全部菌株+++++++++
    57ES3+++++++
    ES35+++++++
    ES37++++
    ES38++++
    ES39++++
    ES45+++
    ES46+++
    ES52++++++
    61ES3++++
    ES35++++
    ES37+
    ES38++
    ES39+
    ES45++
    ES46++
    ES52+++
    65全部菌株
    注:“+”表示存活,“−”表示死亡。处理后原液直接涂布,有菌落存在即为存活,无菌落生长即为死亡;表3同。
    下载: 导出CSV

    表  3  克罗诺杆菌在不同pH下的生长情况

    Table  3.   Growth of Cronobacter spp. at different pH

    pH菌株编号时间(s)
    10306090120
    1.0全部菌株
    1.5ES3++++
    ES35+++
    ES37+++
    ES38++
    ES39+++
    ES45++
    ES46++
    ES52+++
    2.0ES3+++++
    ES35++++
    ES37++++
    ES38++++
    ES39++++
    ES45++++
    ES46+++
    ES52++++
    12.0ES3++++
    ES35++++
    ES37++++
    ES38+++++
    ES39++++
    ES45++++
    ES46++++
    ES52++++
    12.5ES3+
    ES35+
    ES37+
    ES38++
    ES39++
    ES45+
    ES46+
    ES52+
    13.0ES3
    ES35
    ES37
    ES38+
    ES39
    ES45
    ES46
    ES52
    下载: 导出CSV
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  • 收稿日期:  2022-04-07
  • 网络出版日期:  2022-10-21
  • 刊出日期:  2022-11-23

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