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中国精品科技期刊2020

仿刺参雌性生殖腺皂苷的免疫增强活性研究

钟静诗 王共明 张健 刘芳 井月欣 王艺欣

钟静诗,王共明,张健,等. 仿刺参雌性生殖腺皂苷的免疫增强活性研究[J]. 食品工业科技,2022,43(23):378−386. doi:  10.13386/j.issn1002-0306.2022030289
引用本文: 钟静诗,王共明,张健,等. 仿刺参雌性生殖腺皂苷的免疫增强活性研究[J]. 食品工业科技,2022,43(23):378−386. doi:  10.13386/j.issn1002-0306.2022030289
ZHONG Jingshi, WANG Gongming, ZHANG Jian, et al. Study on Immune-enhancing Activity of Gonad Saponins from Female of Apostichopus japonicus[J]. Science and Technology of Food Industry, 2022, 43(23): 378−386. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2022030289
Citation: ZHONG Jingshi, WANG Gongming, ZHANG Jian, et al. Study on Immune-enhancing Activity of Gonad Saponins from Female of Apostichopus japonicus[J]. Science and Technology of Food Industry, 2022, 43(23): 378−386. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2022030289

仿刺参雌性生殖腺皂苷的免疫增强活性研究

doi: 10.13386/j.issn1002-0306.2022030289
基金项目: 山东省现代农业产业技术体系刺参产业创新团队建设项目(SDAIT-22-07);山东省自然科学基金重点项目(ZR2020KC034)。
详细信息
    作者简介:

    钟静诗(1997−),女,硕士研究生,研究方向:食品工程,E-mail:465369582@qq.com

    通讯作者:

    王共明(1988−),男,硕士,工程师,研究方向:食品科学,E-mail:wgmsd105@163.com

    张健(1980−),男,博士,副研究员,研究方向:食品科学,E-mail:zjsd408@163.com

  • 中图分类号: TS201.4

Study on Immune-enhancing Activity of Gonad Saponins from Female of Apostichopus japonicus

  • 摘要: 目的:以仿刺参雌性生殖腺皂苷(gonad saponins from female of Apostichopus japonicus,AGS)为研究对象,探究其免疫增强活性。方法:通过对巨噬细胞吞噬能力和细胞因子的测定,研究AGS的体外免疫调节活性;通过对免疫低下小鼠胸腺、脾脏指数,碳廓清指数与吞噬指数,腹腔巨噬细胞增殖活性,脾细胞增殖活性,自然杀伤细胞(natural killer cell,NK cell)活力,T淋巴细胞亚群水平及小鼠血清细胞因子的测定,分析AGS在小鼠体内的免疫调节活性。结果:体外实验表明,AGS能提高小鼠体外巨噬细胞吞噬能力,促进TNF-α、IL-6、IFN-γ的分泌,且各组与正常组相比差异显著(P<0.05);体内实验表明,与模型组相比,中剂量组和高剂量组AGS均能显著提高小鼠胸腺、脾脏指数、碳廓清指数和吞噬指数(P<0.05),显著增强腹腔巨噬细胞和脾细胞增殖活性(P<0.05),显著增强NK细胞的活性(P<0.05),显著提高CD4+/CD8+水平(P<0.05),显著促进TNF-α、IL-6、IFN-γ的分泌(P<0.05)。结论:AGS在体外和体内都表现出增强免疫的活性。
  • 图  1  AGS对RAW264.7细胞因子的影响

    Figure  1.  Effects of AGS on the release of cytokines in RAW264.7 cells

    注:A:TNF-α;B:IL-6;C:IFN-γ;*表示与正常组相比,差异显著(P<0.05);#表示与LPS组相比,差异显著(P<0.05);图2~图4同。

    图  2  AGS对小鼠巨噬细胞增殖活性的影响

    Figure  2.  Effects of AGS on proliferation of macrophages in mice

    图  3  AGS对小鼠脾细胞增殖活性的影响

    Figure  3.  Effects of AGS on proliferation of splenocytes in mice

    图  4  AGS对小鼠脾细胞中自然杀伤细胞(NK细胞)活力的影响

    Figure  4.  Effects of AGS on NK cell activity of splenocytes in mice

    图  5  AGS对小鼠脾脏T淋巴细胞亚群的影响

    Figure  5.  Effect of AGS on T lymphocyte subsets of spleen in mice

    注:A:正常组;B:模型组;C:低剂量组;D:中剂量组;E:高剂量组。

    图  6  AGS对小鼠血清细胞因子的影响

    Figure  6.  Effects of AGS the release of cytokines in serum of mice

    注:A:TNF-α;B:IL-6;C:IFN-γ;*表示与正常组相比,差异显著(P<0.05);#表示与模型组相比,差异显著(P<0.05)。

    表  1  各组小鼠给药处理表

    Table  1.   Dosing and treatment of mice in each group

    序号组别是否注射环磷酰胺第11~20 d灌胃
    1正常组去离子水
    2模型对照组去离子水
    3低剂量组0.5 mg/kg
    4中剂量组5 mg/kg
    5高剂量组20 mg/kg
    下载: 导出CSV

    表  2  AGS对RAW264.7吞噬能力的影响

    Table  2.   Effects of AGS on the phagocytosis of RAW264.7 cells

    组别吸光度
    正常组0.282±0.014
    200(μg/mL)0.329±0.018*
    500(μg/mL)0.321±0.006*
    1000(μg/mL)0.320±0.007*
    LPS0.338±0.015*
    注:*表示与正常组相比,差异显著(P<0.05)。
    下载: 导出CSV

    表  3  AGS对免疫低下小鼠脏器指数的影响

    Table  3.   Effects of AGS on organ indices in immunocompromised mice

    组别胸腺指数(mg/10 g bw)脾脏指数(mg/10 g bw)
    正常组51.05±6.2749.92±5.25
    模型组29.19±2.31*39.32±2.11*
    低剂量组36.22±0.19*#40.86±0.66*
    中剂量组48.84±4.97#46.80±0.85#
    高剂量组62.03±3.29#47.26±0.14#
    注:*表示与正常组相比,差异显著(P<0.05);#表示与模型组相比,差异显著(P<0.05);表4~表5同。
    下载: 导出CSV

    表  4  AGS对免疫低下小鼠碳廓清指数、吞噬指数的影响

    Table  4.   Effect of AGS on carbon clearance index and phagocytic index in immunocompromised mice

    组别碳廓清指数吞噬指数
    正常组0.0689±0.00190.3551±0.0179
    模型组0.0199±0.0015*0.0993±0.0119*
    低剂量组0.0210±0.0020*0.1110±0.0116*
    中剂量组0.0339±0.0030*#0.1888±0.0069*#
    高剂量组0.0409±0.0014*#0.2489±0.0266*#
    下载: 导出CSV

    表  5  AGS对小鼠脾脏T淋巴细胞亚群的影响

    Table  5.   Effect of AGS on T lymphocyte subsets of spleen in mice

    组别CD4+(%)CD8+(%)CD4+/CD8+
    正常组72.33±0.4021.63±0.643.35±0.11
    模型组56.17±0.57*35.80±0.26*1.57±0.02*
    低剂量组58.10±1.21*27.80±0.87*#2.09±0.11*#
    中剂量组66.23±0.40*#22.13±0.57#2.99±0.09*#
    高剂量组58.43±0.32*#29.50±0.79*#1.98±0.04*#
    下载: 导出CSV
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出版历程
  • 收稿日期:  2022-03-23
  • 网络出版日期:  2022-10-19
  • 刊出日期:  2022-11-23

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