• Scopus
  • CA
  • DOAJ
  • FSTA
  • JST
  • 北大核心期刊
  • 中国科技核心期刊CSTPCD
  • 中国精品科技期刊
  • RCCSE中国核心学术期刊
  • 中国农业核心期刊
  • 中国生物医学文献服务系统SinoMed收录期刊
中国精品科技期刊2020

松茸多糖的体外抗氧化性及对乙醇氧化损伤小鼠作用的研究

沈成龙 陈怡帆 曾英杰 陈炼红

沈成龙,陈怡帆,曾英杰,等. 松茸多糖的体外抗氧化性及对乙醇氧化损伤小鼠作用的研究[J]. 食品工业科技,2022,43(23):371−377. doi:  10.13386/j.issn1002-0306.2022020248
引用本文: 沈成龙,陈怡帆,曾英杰,等. 松茸多糖的体外抗氧化性及对乙醇氧化损伤小鼠作用的研究[J]. 食品工业科技,2022,43(23):371−377. doi:  10.13386/j.issn1002-0306.2022020248
SHEN Chenglong, CHEN Yifan, ZENG Yingjie, et al. Study on Antioxidant Activity of Tricholoma matsutake Polysaccharide in Vitro and Its Effect on Mice Damaged by Ethanol Oxidation[J]. Science and Technology of Food Industry, 2022, 43(23): 371−377. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2022020248
Citation: SHEN Chenglong, CHEN Yifan, ZENG Yingjie, et al. Study on Antioxidant Activity of Tricholoma matsutake Polysaccharide in Vitro and Its Effect on Mice Damaged by Ethanol Oxidation[J]. Science and Technology of Food Industry, 2022, 43(23): 371−377. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2022020248

松茸多糖的体外抗氧化性及对乙醇氧化损伤小鼠作用的研究

doi: 10.13386/j.issn1002-0306.2022020248
基金项目: 西南民族大学中央高校基本科研业务费专项资金项目资助(2022NYXXS013)。
详细信息
    作者简介:

    沈成龙(2000−),男,硕士研究生,研究方向:食品加工与安全,E-mail:1402289402@qq.com

    通讯作者:

    陈炼红(1967−),女,硕士,教授,研究方向:民族食品资源开发与研究,E-mail:lianhong_chen@163.com

  • 中图分类号: TS201.4

Study on Antioxidant Activity of Tricholoma matsutake Polysaccharide in Vitro and Its Effect on Mice Damaged by Ethanol Oxidation

  • 摘要: 本文以超氧阴离子自由基(Superoxide anion radicals,O2 · )、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate),ABTS)自由基清除能力、总还原能力为指标研究松茸多糖(Tricholoma matsutake polysaccharide,TMP)体外抗氧化能力;建立乙醇氧化损伤小鼠模型,通过测定小鼠血清中谷丙转氨酶(Alanine transaminase,ALT)、谷草转氨酶(Aspartate transaminase,AST)和碱性磷酸酶(Alkaline phosphatase,AKP)的活力以及肝脏中过氧化氢酶(Catalase,CAT)、丙二醛(Malondialdehyde,MDA)、超氧化物歧化酶(Superoxide dismutase,SOD)和谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-Px)的水平研究松茸多糖的体内抗氧化活性。体外抗氧化结果表明,松茸多糖对超氧阴离子自由基和ABTS自由基有较好的清除能力,当浓度为1 mg/mL时,清除率分别为56.67%和96.60%,而松茸多糖的总还原能力较低,当浓度为1 mg/mL,吸光值为0.163;动物实验结果表明,连续灌胃四周后,与模型对照组相比,松茸多糖可以使小鼠血清中的ALT、AST和AKP活力显著降低(P<0.05),使肝脏中CAT、SOD和GSH-Px的水平显著升高(P<0.05),MDA的含量显著降低(P<0.05),且呈剂量依赖性。研究表明松茸多糖有一定的抗氧化活性,可为松茸多糖的后续开发应用提供思路。
  • 图  1  松茸多糖对超氧阴离子自由基的清除能力

    Figure  1.  The scavenging ability of Tricholoma matsutake polysaccharides on superoxide anion free radicals

    图  2  松茸多糖的总还原能力

    Figure  2.  The total reducing power of Tricholoma matsutake polysaccharides

    图  3  松茸多糖对ABTS自由基的清除能力

    Figure  3.  The ability of Tricholoma matsutake polysaccharides to scavenge ABTS free radicals

    图  4  小鼠肝脏切片(400×)

    Figure  4.  Mouse liver section(400×)

    表  1  实验小鼠分组及处理

    Table  1.   Grouping and treatment of experimental mice

    组别灌胃试剂灌胃剂量
    空白对照组生理盐水0.3 mL
    模型对照组生理盐水0.3 mL
    阳性对照组VC100 mg/kg
    低剂量组松茸多糖溶液100 mg/kg
    中剂量组松茸多糖溶液200 mg/kg
    高剂量组松茸多糖溶液400 mg/kg
    下载: 导出CSV

    表  2  小鼠体重变化

    Table  2.   Changes in body weight of mice

    组别小鼠体重(g)
    0 d7 d14 d21 d28 d
    空白对照组30.4±0.3231.9±0.4233.7±0.5134.8±0.3736.0±0.22
    模型对照组29.8±0.5532.3±0.6233.1±0.3534.3±0.4435.7±0.29
    阳性对照组30.2±0.4532.1±0.5833.4±0.6134.9±0.4535.8±0.71
    低剂量组30.3±0.3732.3±0.3233.8±0.2934.9±0.5435.6±0.27
    中剂量组30.5±0.2831.8±0.3333.5±0.5635.0±0.2736.2±0.61
    高剂量组30.1±0.4332.5±0.5133.6±0.3634.9±0.4136.1±0.52
    下载: 导出CSV

    表  3  松茸多糖对小鼠血清中ALT、AST、AKP活力的影响

    Table  3.   Effect of Tricholoma matsutake polysaccharides on the activities of ALT, AST and AKP in the serum of mice

    组别剂量
    (mg/kg)
    ALT
    (U/L)
    AST
    (U/L)
    AKP
    (U/L)
    空白对照组014.61±0.5932.43±1.3756.26±3.28
    模型对照组028.33±1.29#60.19±1.72#182.28±9.42#
    阳性对照组10017.76±1.02*37.22±1.02*75.89±4.78*
    低剂量组10024.56±1.06*&45.64±1.27*&147.65±11.92*&
    中剂量组20022.34±1.39*&42.96±1.43*&136.02±10.21*&
    高剂量组40020.16±1.03*40.22±1.04*109.88±6.28*&
    注:#:与空白对照组比较,差异显著(P<0.05); *:与模型对照组比较,差异显著(P<0.05); &:与阳性对照组比,差异显著(P<0.05);表4同。
    下载: 导出CSV

    表  4  松茸多糖对小鼠肝脏中CAT、MDA、SOD和GSH-Px水平的影响

    Table  4.   Effects of Tricholoma matsutake polysaccharides on the levels of CAT, MDA, SOD and GSH-Px in the liver of mice

    组别剂量
    (mg/kg)
    CAT
    (U/mg prot)
    SOD
    (U/mg prot)
    MDA
    (nmol/mg prot)
    GSH-Px
    (U/mg prot)
    空白对照组097.03±2.84152.83±2.884.60±0.3878.60±3.83
    模型对照组044.41±1.63#110.58±2.17#10.61±0.27#41.67±1.23#
    阳性对照组10089.05±3.24*150.88±2.65*5.23±0.22*74.46±2.98*
    低剂量组10056.73±1.67*&117.44±1.89&8.30±0.66*&48.55±1.09&
    中剂量组20062.45±2.21*&120.89±1.22*&8.16±0.94*&47.93±1.94&
    高剂量组40072.42±1.78*&144.52±2.51*6.29±0.44*68.47±2.06*
    下载: 导出CSV
  • [1] 赵顺义. 珍稀名贵食用菌—松茸[J]. 养生月刊,2017,38(6):518−519. [ZHAO S Y. Rare and rare edible fungi—matsutake[J]. Health Preservation Monthly,2017,38(6):518−519. doi:  10.3969/j.issn.1671-1734.2017.06.012
    [2] 李建文, 杨永平, 毕迎凤, 等. 松茸主要产地的遗传多样性研究[C]. 中国菌物学会2016年学术年会论文摘要集. 2016.

    LI J W, YANG Y P, BI Y F, et al. Genetic diversity of Tricholoma matsutake from major producing areas[C]. Proceedings of the 2016 Annual Meeting of Chinese Fungi Society. 2016.
    [3] DENG M, WANG J, LI Y L, et al. The impact of polyphenols extracted from Tricholoma matsutake on UVB-induced photoaging in mouse skin[J]. Journal of Cosmetic Dermatology,2021,21(2):781−793.
    [4] 王秋果. 野生松茸有效成分活性研究及风味产品的研制[D]. 成都: 成都大学, 2020.

    WANG Q G. Study on active ingredients of wild matsutake and development of flavored products[D]. Chengdu: Chengdu University, 2020.
    [5] KIM J Y, BYEON S E, LEE Y G, et al. Immunostimulatory activities of polysaccharides from liquid culture of pine-mushroom Tricholoma matsutake[J]. Journal of Microbiology and Biotechnology,2008,18(1):95−103.
    [6] DING X, HOU W, ZHONG J, et al. Anti-microorganism, anti-tumor, and immune activities of a novel polysaccharide isolated from Tricholoma matsutake[J]. Pharmacognosy Magazine,2013,9(35):244−249. doi:  10.4103/0973-1296.113278
    [7] YANG H R, CHEN L H, ZENG Y J. Structure, antioxidant activity and in vitro hypoglycemic activity of a polysaccharide purified from Tricholoma matsutake[J]. Foods,2021,10(9):2184. doi:  10.3390/foods10092184
    [8] YOU L, GAO Q, FENG M, et al. Structural characterisation of polysaccharides from Tricholoma matsutake and their antioxidant and antitumour activities[J]. Food Chemistry,2013,138(4):2242−2249. doi:  10.1016/j.foodchem.2012.11.140
    [9] HU Y, HE Y, NIU Z, et al. A review of the immunomodulatory activities of polysaccharides isolated from Panax species[J]. Journal of Ginseng Research,2022,46(1):23−32. doi:  10.1016/j.jgr.2021.06.003
    [10] PURUSHOTHAMAN A, TEENA R K S, JACOB J M, et al. Curcumin analogues with improved antioxidant properties: A theoretical exploration[J]. Food Chemistry,2021,373(PB):131499.
    [11] 周静. 天然抗氧化物质的提取、分离及活性研究[D]. 杭州: 浙江大学, 2008.

    ZHOU J. Study on extraction, isolation and activity of natural antioxidants [D]. Hangzhou: Zhejiang University, 2008.
    [12] MU S, YANG W, HUANG G. Antioxidant activities and mechanisms of polysaccharides[J]. Chemical Biology & Drug Design,2020,97(3):628−632.
    [13] CHEN Y, DU X J, ZHANG Y, et al. Ultrasound extraction optimization, structural features, and antioxidant activity of polysaccharides from Tricholoma matsutake[J]. Journal of Zhejiang University-Science B (Biomedicine & Biotechnology),2017,18(8):674−684.
    [14] 吴杨洋, 田淑雨, 鹿士峰, 等. 不同提取方法对松茸多糖理化性质及抗氧化活性的影响[J]. 食品安全质量检测学报,2018,9(19):5164−5170. [WU Y Y, TIAN S Y, LU S F, et al. Effects of different extraction methods on physicochemical properties and antioxidant activity of Tricholoma matsutake polysaccharides[J]. Journal of Food Safety,2018,9(19):5164−5170. doi:  10.3969/j.issn.2095-0381.2018.19.026
    [15] DING X, TANG J, CAO M, et al. Structure elucidation and antioxidant activity of a novel polysaccharide isolated from Tricholoma matsutake[J]. International Journal of Biological Macromolecules,2010,47(2):271−275. doi:  10.1016/j.ijbiomac.2010.04.010
    [16] 刘刚, 王辉, 张洪. 松茸多糖对D-半乳糖所致小鼠衰老模型的影响[J]. 中国药理学通报,2012,28(10):1439−1442. [LIU G, WANG H, ZHANG H. Effects of Tricholoma matsutake polysaccharides on D-galactose induced aging in mice[J]. Chinese Pharmacological Bulletin,2012,28(10):1439−1442.
    [17] 王静杰, 杜鑫, 钟强, 等. 超声辅助酶法制备海参性腺多糖的结构特性及抗氧化活性[J]. 食品科学,2021,42(21):97−104. [WANG J J, DU X, ZHONG Q, et al. Structural characteristics and antioxidant activity of polysaccharides prepared from sea cucumber gonad by ultrasound-assisted enzymatic hydrolysis[J]. Food Science,2021,42(21):97−104. doi:  10.7506/spkx1002-6630-20201215-186
    [18] 孔沛筠, 常雅宁, 聂嘉睿, 等. 木耳多糖提取工艺优化及其体外抗氧化活性研究[J]. 食品与药品,2018,20(3):187−193. [KONG P Y, CHANG Y N, NIE J R, et al. Optimization of extraction process and antioxidant activity of Auricularia auriculata polysaccharide[J]. Food & Drug,2018,20(3):187−193. doi:  10.3969/j.issn.1672-979X.2018.03.006
    [19] 何坤明, 王国锭, 白新鹏, 等. 山茱萸籽多糖分离纯化、结构表征及抗氧化活性[J]. 食品科学,2021,42(19):81−88. [HE K M, WANG G D, BAI X P, et al. Isolation, purification, structure characterization and antioxidant activity of Cornus officinalis seed polysaccharides[J]. Food Science,2021,42(19):81−88. doi:  10.7506/spkx1002-6630-20201111-109
    [20] 国家食品药品监督管理总局. 国食药监保化[2012]107号《关于印发抗氧化功能评价方法等9个保健功能评价方法的通知》[EB/OL]. (2012-04-23) [2017-07 -18]. http://www.sda.gov.cn/WS01/CL0847/71257.html.

    China Food and Drug Administration. State food and drug administration [2012] No. 107《About printing nine health care functions such as antioxidant function evaluation method evaluation method of circular》[EB/OL]. (2012-04-23) [2017-07-18]. http://www.sda.gov.cn/WS01/CL0847/71257.html.
    [21] 中华人民共和国卫生部. 保健食品检验与评价技术规范[M]. 北京: 化学工业出版社, 2003: 43−71

    Ministry of Health of the People's Republic of China. Technical specification for inspection and evaluation of health food[M]. Beijing: Chemical Industry Press, 2003: 43−71.
    [22] 董浩迪. 黄秋葵黄酮提取分离及其降血糖活性研究[D]. 天津: 天津科技大学, 2019.

    DONG H D. Extraction, separation and hypoglycemic activity of flavonoids from Okra [D]. Tianjin: University of Science and Technology, 2019.
    [23] 付昊, 龙虎, 蔡自建, 等. 熟地黄多糖的体内抗氧化活性研究[J]. 食品研究与开发,2019,40(4):57−61. [FU H, LONG H, CAI Z J, et al. Study on antioxidant activity of polysaccharides from Rehmannia glutinosa[J]. Food Research and Development,2019,40(4):57−61. doi:  10.3969/j.issn.1005-6521.2019.04.011
    [24] 彭勇胜. 姬松茸多糖的制备及其抗氧化、保肝作用研究[D]. 武汉: 华中农业大学, 2011.

    PENG Y S. Preparation of polysaccharides from Agaricus blazei and its antioxidant and liver protection effects [D]. Wuhan: Huazhong Agricultural University, 2011.
    [25] 张玉, 张绵松, 史亚萍, 等. 铜藻活性组分多糖的体外抗氧化性研究[J]. 食品研究与开发,2018,39(6):12−18. [ZHANG Y, ZHANG M S, SHI Y P, et al. Comparison on antioxidant activity of polysaccharide fraction from Sargassum horneri in vitro[J]. Food Research and Development,2018,39(6):12−18. doi:  10.3969/j.issn.1005-6521.2018.06.003
    [26] 张天凤, 张振山, 王帅, 等. 亚麻籽粕提取物抗氧化成分及抗氧化活性研究[J]. 粮食与油脂,2021,34(12):137−141. [ZHANG T F, ZHANG Z S, WANG S, et al. Study on antioxidant components and antioxidant activity of flaxseed meal extract[J]. Grain and Oil,2021,34(12):137−141. doi:  10.3969/j.issn.1008-9578.2021.12.032
    [27] 饶雪甜. 黑果腺肋花楸体外消化模拟及其原花青素性质和应用研究[D]. 广州: 华南理工大学, 2020.

    RAO X T. Study on in vitro digestion simulation of Aronia melanocarpa and properties and application of its proanthocyanidins[D]. Guangzhou: South China University of Technology, 2020.
    [28] 李艺萌, 李文芝, 钟瑞芳, 等. 黑菇多糖的提取工艺优化及理化性质和抗氧化活性研究[J]. 河南工业大学学报(自然科学版),2020,41(3):19−26. [LI Y M, LI W Z, ZHONG R F, et al. Study on extraction process optimization, physicochemical properties, and antioxidant activity of polysaccharide from Russula adusta (Pers.) Fr

    J]. Journal of Henan University of Technology (Natural Science Edition),2020,41(3):19−26.
    [29] 许女, 贾瑞娟, 陈旭峰, 等. 鸡腿菇子实体多糖的体内、体外抗氧化活性[J]. 中国食品学报,2019,19(1):34−40. [XU N, JIA R J, CHEN X F, et al. Antioxidant activity in vitro andin vivo of polysaccharide from Coprinus comatus[J]. Journal of Food Science and Technology,2019,19(1):34−40. doi:  10.16429/j.1009-7848.2019.01.005
    [30] 董博斐, 彭文欣, 杨凤霞, 等. 红平菇胞外多糖体外抗氧化及对高血脂小鼠体内抗氧化能力探究[J]. 食品工业科技,2021,42(5):305−310. [DU B F, PENG W X, YANG F X, et al. Study on the antioxidant capacity of extracellular polysaccharides of Pleurotus djamor in vitro and in hyperlipidemia mice[J]. Science and Technology of Food Industry,2021,42(5):305−310.
    [31] 王箴言, 夏晴, 王玉, 等. 桦褐孔菌不同多糖组分的体内、外抗氧化活性[J]. 中国食品学报,2021,21(8):152−158. [WANG Z Y, XIA Q, WANG Y, et al. Antioxidant activity of different polysaccharide components of Inonotus obliquus in vitro and in vivo[J]. Chinese Journal of Food Science,2021,21(8):152−158. doi:  10.16429/j.1009-7848.2021.08.014
    [32] 庄伟. 黑木耳多糖的提取、结构解析及活性功能研究[D]. 上海: 华东理工大学, 2020.

    ZHUANG W. Study on extraction, structural analysis and active function of polysaccharide isolated from Auricularia auricula-judae (Bull)[D]. Shanghai: East China University of Science and Technology, 2020.
    [33] LI X X, JIANG Z H, ZHOU B, et al. Hepatoprotective effect of gastrodin against alcohol-induced liver injury in mice[J]. Journal of Physiology and Biochemistry,2018,75(1):29−37.
    [34] AGRAWAL S, DHIMAN R K, LIMDI J K. Evaluation of abnormal liver function tests[J]. Postgraduate Medical Journal,2016,92(1086):223−234. doi:  10.1136/postgradmedj-2015-133715
    [35] 欧志强. 碱性磷酸酶(ALP)在妊娠晚期的监测和变化分析[J]. 医学检验与临床,2017,28(8):43−44. [OU Z Q. Monitoring and analysis of alkaline phosphatase (ALP) in late pregnancy[J]. Clin Exp Clin,2017,28(8):43−44. doi:  10.3969/j.issn.1673-5013.2017.08.013
    [36] 梁强. 连钱草抗泌尿系草酸钙结石的药理作用及其机制研究[D]. 兰州: 兰州大学, 2017.

    LIANG Q. Study on pharmacological effects and mechanism of Limonium chinense against urinary calcium oxalate stones[D]. Lanzhou: Lanzhou University, 2017.
    [37] OWOJUYIGBE O S, LARBIE C, FIREMPONG C K, et al. Hura crepitans stem bark extract: A potential remedy to sub-acute liver damage[J]. Journal of Ethnopharmacology,2022,284:114768. doi:  10.1016/j.jep.2021.114768
    [38] HUANG D, LI C, CHEN Q, et al. Identification of polyphenols from Rosa roxburghii Tratt pomace and evaluation of in vitro and in vivo antioxidant activity[J]. Food Chemistry,2022,377:131922. doi:  10.1016/j.foodchem.2021.131922
    [39] LI C X, SHEN L R. New observations on the effect of Camellia oil on fatty liver disease in rats[J]. Journal of Zhejiang University. Science. B,2020,21(8):657−667.
    [40] 吴奇辉. 紫马铃薯花色苷分离纯化及降脂减肥活性研究[D]. 杭州: 浙江大学, 2014.

    WU Q H. Isolation and purification of anthocyanins from purple potato and their lipid-reducing activity[D]. Hangzhou: Zhejiang University, 2014.
  • 加载中
图(4) / 表(4)
计量
  • 文章访问数:  63
  • HTML全文浏览量:  10
  • PDF下载量:  9
  • 被引次数: 0
出版历程
  • 收稿日期:  2022-02-28
  • 网络出版日期:  2022-10-21
  • 刊出日期:  2022-11-23

目录

    /

    返回文章
    返回

    重要通知

    1、快速见刊:客座主编专栏征稿-食源性功能物质挖掘及评价
           2、喜讯 :《食品工业科技》被DOAJ数据库收录!
           3喜报:《食品工业科技》世界期刊影响力稳居Q2区
           4、祝贺:《食品工业科技》中国期刊影响力稳居Q1第二名