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中国精品科技期刊2020

骏枣多糖的分离纯化、结构表征及抗氧化活性研究

赵建成 刘慧燕 方海田

赵建成,刘慧燕,方海田. 骏枣多糖的分离纯化、结构表征及抗氧化活性研究[J]. 食品工业科技,2022,43(23):71−78. doi:  10.13386/j.issn1002-0306.2022020076
引用本文: 赵建成,刘慧燕,方海田. 骏枣多糖的分离纯化、结构表征及抗氧化活性研究[J]. 食品工业科技,2022,43(23):71−78. doi:  10.13386/j.issn1002-0306.2022020076
ZHAO Jiancheng, LIU Huiyan, FANG Haitian. Study on Isolation, Purification, Structure Characterization and Antioxidant Activity of Polysaccharide from Zizyphus jujuba cv. Junzao[J]. Science and Technology of Food Industry, 2022, 43(23): 71−78. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2022020076
Citation: ZHAO Jiancheng, LIU Huiyan, FANG Haitian. Study on Isolation, Purification, Structure Characterization and Antioxidant Activity of Polysaccharide from Zizyphus jujuba cv. Junzao[J]. Science and Technology of Food Industry, 2022, 43(23): 71−78. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2022020076

骏枣多糖的分离纯化、结构表征及抗氧化活性研究

doi: 10.13386/j.issn1002-0306.2022020076
基金项目: 宁夏回族自治区重点研发计划项目(2018BBF02008);宁夏食品微生物应用技术与安全控制重点实验室平台建设项目(2021JCTJ0030、2019YDDF0062)资助。
详细信息
    作者简介:

    赵建成(1996−),女,硕士研究生,研究方向:健康食品生物制造,E-mail:1204684666@qq.com

    通讯作者:

    刘慧燕(1977−),女,硕士,副教授,研究方向:农产品生物加工,E-mail:liuhy@nxu.edu.cn

    方海田(1978−),男,博士,教授,研究方向:健康食品生物制造,E-mail:fanght@nxu.edu.cn

  • 中图分类号: TS201.1

Study on Isolation, Purification, Structure Characterization and Antioxidant Activity of Polysaccharide from Zizyphus jujuba cv. Junzao

  • 摘要: 本实验以骏枣为原料,对骏枣多糖的分离纯化、结构表征及抗氧化活性进行研究。通过水提醇沉得骏枣粗多糖HZPC,再经DEAE-52阴离子层析柱和Sephadex G-100葡聚糖凝胶柱分离纯化获得精制骏枣多糖组分HZPC-2。利用高效凝胶色谱法(HPGPC)和离子色谱法(IC)测定HZPC-2的分子量及单糖构成,紫外光谱、红外光谱和扫描电镜研究HZPC-2的结构特征,最后对HZPC-2的抗氧化活性进行评估。结果表明:HZPC-2为α-吡喃葡萄苷骨架的中性多糖(JDP-N),分子量为3.25×104 Da,是由鼠李糖(Rha)、阿拉伯糖(Ara)、半乳糖(Gal)、葡萄糖(Glc)、木糖(Xyl)、甘露糖(Man)和半乳糖醛酸(GalA)构成,其摩尔比为0.05:0.34:0.29:0.15:0.08:0.02:0.06。扫描电子显微镜观察结果显示,HZPC-2表面呈沟壑状且朝四面延伸,四周嵌着孔状结构。体外抗氧化试验表明,HZPC-2对三种自由基清除活性的最强的是DPPH自由基,其次是羟自由基,最弱的是ABTS自由基,这可作为潜在抗氧化剂以及为开发具有骏枣多糖功能的食品提供研究基础。
  • 图  1  HZPC的DEAE-52洗脱曲线

    Figure  1.  DEAE-52 elution curve of HZPC

    图  2  HZPC-2的Sephadex G-100 洗脱曲线

    Figure  2.  Sephadex G-100 elution curve of HZPC-2

    图  3  HZPC-2的紫外吸收光谱(a)和傅里叶红外吸收光谱图(b)

    Figure  3.  UV (a) and FTIR (b) absorption spectra of HZPC-2

    图  4  HZPC-2单糖构成分析

    Figure  4.  Analysis of monosaccharide composition of HZPC-2

    注:Rha:鼠李糖;Ara:阿拉伯糖;Gal:半乳糖;Glc:葡萄糖;Xyl:木糖;Man:甘露糖;GalA:半乳糖醛酸。

    图  5  HZPC-2的SEM 图像

    Figure  5.  SEM image of HZPC-2

    图  6  HZPC-2的抗氧化活性

    Figure  6.  Antioxidant activity of HZPC-2

    表  1  HZPC-2单糖构成分析

    Table  1.   Analysis of monosaccharide composition of HZPC-2

    组别标准品HZPC-2样品摩尔比
    保留时间
    (min)
    峰面积
    (%)
    保留时间
    (min)
    峰面积
    (%)
    岩藻糖Fuc6.057.976.050.00
    盐酸氨基半乳糖GalN11.2710.7611.270.00
    鼠李糖Rha11.768.1811.761.190.05
    阿拉伯糖Ara12.636.5512.637.490.34
    盐酸氨基葡萄糖GlcN14.0921.1614.090.00
    半乳糖Gal15.938.2015.937.230.29
    葡萄糖Glc18.0310.0218.034.480.15
    N-乙酰-D氨基葡萄糖GlcNAc19.663.2119.660.00
    木糖Xyl21.035.7921.031.170.08
    甘露糖Man21.626.2721.620.440.02
    果糖Fru24.865.4624.860.00
    核糖Rib27.7618.8627.760.00
    半乳糖醛酸GalA45.428.4245.421.650.06
    古罗糖醛酸GulA46.1513.2546.150.00
    葡萄糖醛酸GlcA48.6310.8248.630.00
    甘露糖醛酸ManA50.9825.7150.980.00
    下载: 导出CSV
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  • 收稿日期:  2022-02-14
  • 网络出版日期:  2022-10-20
  • 刊出日期:  2022-11-23

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