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中国精品科技期刊2020

热处理和蛋白浓度对肌原纤维蛋白乳液的稳定性和流变特性的影响

郝梦 毛书灿 周志 汪兰 熊光权 石柳

郝梦,毛书灿,周志,等. 热处理和蛋白浓度对肌原纤维蛋白乳液的稳定性和流变特性的影响[J]. 食品工业科技,2022,43(23):56−63. doi:  10.13386/j.issn1002-0306.2022020066
引用本文: 郝梦,毛书灿,周志,等. 热处理和蛋白浓度对肌原纤维蛋白乳液的稳定性和流变特性的影响[J]. 食品工业科技,2022,43(23):56−63. doi:  10.13386/j.issn1002-0306.2022020066
HAO Meng, MAO Shucan, ZHOU Zhi, et al. Effect of Heat Treatment and Protein Concentration on the Stability and Rheological Properties of Myofibrillar Protein Emulsion[J]. Science and Technology of Food Industry, 2022, 43(23): 56−63. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2022020066
Citation: HAO Meng, MAO Shucan, ZHOU Zhi, et al. Effect of Heat Treatment and Protein Concentration on the Stability and Rheological Properties of Myofibrillar Protein Emulsion[J]. Science and Technology of Food Industry, 2022, 43(23): 56−63. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2022020066

热处理和蛋白浓度对肌原纤维蛋白乳液的稳定性和流变特性的影响

doi: 10.13386/j.issn1002-0306.2022020066
基金项目: 湖北省自然科学基金(2020CFB466);湖北省农业科技创新中心重大科技研发项目(2020-620-000-002-03)。
详细信息
    作者简介:

    郝梦(1997−),女,硕士研究生,研究方向:水产品加工,E-mail:haomeng_1997@163.com

    通讯作者:

    石柳(1988−),女,博士,助理研究员,研究方向:水产品加工与综合利用,E-mail:shiliu@hbaas.com

  • 中图分类号: TS254.9

Effect of Heat Treatment and Protein Concentration on the Stability and Rheological Properties of Myofibrillar Protein Emulsion

  • 摘要: 为了获得稳定的肌原纤维蛋白乳液,本文以冷冻白鲢鱼糜为原料提取肌原纤维蛋白,研究热处理(85 ℃ 10 min)和蛋白浓度(5、10、15、20、25 mg/mL)对肌原纤维蛋白溶液聚集比例、粒径、电位和微观结构等及对大豆油-肌原纤维蛋白乳液结构、表观粘度和色度的影响。结果表明:热处理使肌原纤维蛋白溶液中的蛋白发生聚集,使乳液粒径增大,表观粘度减小,乳液的L*b*增加,疏水性减弱。随蛋白浓度的增加,未热处理组和热处理组的肌原纤维蛋白溶液的表观粘度逐渐增大,电位值波动上升,且分别在蛋白浓度为10和25 mg/mL时平均粒径最小。随蛋白浓度增加,肌原纤维蛋白乳液中参与乳化的油滴数量增多,油滴粒径减小,聚结程度减小。因此,在油相比为0.6,蛋白浓度为10~20 mg/mL时,热处理组的肌原纤维蛋白乳液液滴小而分散,表观粘度低,乳液稳定性高。本研究对开发稳定的肌原纤维蛋白乳液具有重要意义。
  • 图  1  不同浓度下的MP组和MPA组肌原纤维蛋白溶液的SDS-PAGE凝胶电泳

    Figure  1.  SDS-PAGE gel electrophoresis of myofibrillar protein solution in MP and MPA groups at different concentrations

    图  2  不同蛋白浓度下热处理后的MPA组蛋白聚集比例

    Figure  2.  Protein aggregation ratio of MPA group after heat treatment at different protein concentrations

    注:不同字母表示差异性显著(P<0.05),图4同。

    图  3  热处理对肌原纤维蛋白溶液粒径分布的影响

    Figure  3.  Effect of heat treatment on particle size distribution of myofibrillar protein solution

    注:A:MP,未加热组;B:MPA,加热组。

    图  4  热处理对肌原纤维蛋白溶液电位的影响

    Figure  4.  Effect of heat treatment on myofibrillar protein solution potential

    图  5  热处理对MP(A)和MPA组(B)肌原纤维蛋白溶液粘度的影响

    Figure  5.  Effect of heat treatment on myofibrillar protein solution viscosity in MP (A) and MPA groups (B)

    图  6  不同浓度和油相比的MP组乳液(A)和MPA组乳液(B)24 h静置情况

    Figure  6.  Emulsion of MP group (A) and MPA group (B) standing for 24 h with different concentrations and oil

    图  7  肌原纤维蛋白乳液0和24 h(4 ℃放置)放置正拍图

    Figure  7.  Positive pictures of myofibrillar protein emulsion at 0 and 24 h (placed at 4 ℃)

    图  8  热处理对MP和MPA组乳液微观形貌的影响

    Figure  8.  Influence of heat treatment on micromorphology of emulsion in MP and MPA groups

    图  9  MP(A)和MPA组(B)乳液的红外光谱图

    Figure  9.  Infrared spectrogram of MP (A) and MPA (B) emulsion groups

    图  10  MP(A)和MPA组(B)肌原纤维蛋白乳液粘度随剪切速率的变化曲线

    Figure  10.  Myofibrillar protein emulsion viscosity curve with shear rate in MP (A) and MPA groups (B)

    表  1  不同浓度下MP组和MPA组肌原纤维蛋白溶液的平均粒径(D(4,3)

    Table  1.   Average particle size of myofibrillar protein solution in MP group and MPA group at different concentrations (D(4,3))

    蛋白浓度(mg/mL)510152025
    MP(μm)152.8±6.5b92.7±9.6c181.7±10.7a163.4±4.9b148.8±7.3b
    MPA(μm)238.3±5.7a179.2±2.4c198.3±9.7b183.3±6.2c166.1±2.3d
    注:同行不同小写字母表示差异性显著(P<0.05)。
    下载: 导出CSV

    表  2  热处理对MP和MPA组乳液色度的影响

    Table  2.   Effect of heat treatment on chromaticity of emulsion in MP and MPA groups

    样品蛋白浓度(mg/mL)L*a*b*
    MP561.6±0.6e−1.8±0.1c3.5±0.3c
    1078.2±0.3c−1.9±0.1d6.0±0.3a
    1581.1±0.1b−1.6±0.0ab5.2±0.2b
    2087.5±0.2a−1.6±0.0ab5.1±0.0b
    2563.8±0.3d−1.5±0.1a2.3±0.4d
    MPA550.4±1.8d−1.3±0.0a1.7±0.1c
    1082.2±0.8c−1.9±0.0c5.5±0.1b
    1584.8±0.6b−1.7±0.0b5.3±0.0b
    2087.5±0.1ab−1.9±0.0c6.0±0.1a
    2589.3±1.0a−1.9±0.0c6.2±0.1a
    注:同列不同小写字母表示同一样品不同浓度间差异性显著(P<0.05)。
    下载: 导出CSV
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  • 收稿日期:  2022-02-14
  • 网络出版日期:  2022-10-20
  • 刊出日期:  2022-11-23

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