葡萄糖无酶快检技术研究进展

王艳; 程美佳; 谢金晖; 刘天娇; 李虹佳; 辛嘉英

doi:10.13386/j.issn1002-0306.2022020019

葡萄糖无酶快检技术研究进展

doi: 10.13386/j.issn1002-0306.2022020019

1.
哈尔滨商业大学食品科学与工程重点实验室，黑龙江哈尔滨 150076
2.
中国科学院兰州化学物理研究所，羰基合成与选择氧化国家重点实验室，甘肃兰州 730000

基金项目: 黑龙江省自然科学基金（LH2021C051）；哈尔滨商业大学青年创新人才项目青年后备人才专项（2019CX33）；黑龙江省“百千万”工程科技重大专项支撑行动计划（2021ZX12B07）的子课题（2021ZX12B07-2）；中央支持地方高校改革发展资金人才培养支持计划项目（高水平人才）（304017）。

详细信息

作者简介:
王艳（1984−），女，博士，副教授，研究方向：食品生物技术和微生物发酵技术研究，E-mail：wangyan_123456@163.com

中图分类号: Q814.2
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- 文章访问数: 84
- HTML全文浏览量: 34
- PDF下载量: 27
- 被引次数: 0
出版历程
- 收稿日期: 2022-02-10
- 网络出版日期: 2022-10-19
- 刊出日期: 2022-11-23

Research Progress in Glucose Enzyme-free Rapid Detection Technology

1.
Key Laboratory for Food Science and Engineering, Harbin University of Commerce, Harbin 150076, China
2.
State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China

摘要

摘要: 葡萄糖的检测在食品与医疗行业中尤为重要，电化学无酶检测与可视化检测因其测量快、便捷、操作简单而广泛应用于食品工业、生物检测、医疗卫生等领域，并成为研究人员关注的热点。本文综述了国内外的葡萄糖无酶快检技术，对无酶葡萄糖传感技术的研究进展进行了总结，其中，构建模拟酶技术在电化学与可视化检测中均得到了广泛研究，讨论了它们在临床医学与食品分析葡萄糖检测中的应用，并对葡萄糖无酶检测技术的未来研究方向进行了展望，为后续开发研究及在医疗和农业行业应用提供了理论依据。
- 葡萄糖无酶检测 /
- 电化学检测 /
- 可视化检测 /
- 模拟酶催化检测
Abstract: Glucose detection is particularly important in the food and medical industry. Electrochemical enzyme-free detection and visual detection are widely used in the food industry, biological detection, medical and health and other fields because of its fast, convenient measurement and simple operation. And it has become a hot topic for researchers. This paper summarizes the technology of enzyme-free fast glucose detection at home and abroad. The research progress of enzyme-free glucose sensing technology is reviewed. And the application of these technology is discussed. Among them, the construction simulation enzyme technology in the electrochemical and visual detection are extensive research, discusses their application in clinical medicine and food analysis glucose detection, and the future of glucose enzyme-free detection technology research direction is prospected, for the subsequent development research and application in medical and agricultural industry provides a theoretical basis.
- glucose enzyme-free detection /
- electrochemical detection /
- visual detection /
- simulated enzyme catalytic detection

HTML全文

图 1 基于AuNPs的自限增长系统示意图^[7]

Figure 1. Schematic diagram of AuNPs-based self-limiting growth system^[7]

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图 2 在ITO/PbS/SiO₂/AuNPs电极上检测葡萄糖的PEC策略示意图^[9]

Figure 2. Schematic illustration of the PEC strategy for detection of glucose at ITO/PbS/SiO₂/AuNPs electrode^[9]

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图 3 CuOx@BPC/GCE葡萄糖非酶检测机理图^[23]

Figure 3. CuOx@ BPC/GCE glucose non-enzyme detection mechanism diagram^[23]

注：参比电极使用饱和甘汞电极（SCE）。

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图 4 在H₂O₂存在时，GO-COOH作为过氧化物模拟酶氧化TMB机理图^[67]

Figure 4. Mechanism diagram of oxidation of TMB by GO-COOH as a peroxide mimicking enzyme in the presence of hydrogen peroxide^[67]

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图 5 基于Au@Ag的双金属核壳纳米粒子（Au@AgNPs）和碳纳米点（C-dots）的双信号葡萄糖检测示意图^[70]

Figure 5. Schematic representation of dual-signal glucose detection using Au @Ag-based bimetal nuclear-shell nanoparticles （Au@Ag NPs） and carbon nanodots （C-dots）^[70]

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表 1 CuO基非酶促葡萄糖传感器性能

Table 1. CuO-based non-enzymatic glucose sensor performance

电极材料	灵敏度（µA mmol⁻¹cm⁻²）	线性范围（mmol/L）	检出限（µmol/L）	缓冲溶液	参考文献
CuO纳米盘/SPCE	627.3	0.002~2.5	0.2	0.1 mol/L KOH	[24]
Cu₃（BTC）₂-衍生CuO	1523.5	<1.25	1	0.1mol/L NaOH	[29]
CuO纳米花/GCE	2634.4	5×10⁻⁴~2.67	0.26	0.05 mol/L NaOH	[25]
CuO-PANI	2800	2.5×10⁻⁴~0.28	0.24	0.1mol/L NaOH	[30]
-NF/FTO	1359	0.28~4.6	0.24	0.1mol/L NaOH	[30]
CuO饼干状/SPCE	308.7	0.0005~4.03	0.1	0.1 mol/L NaOH	[31]
3D-KSC/CuO @C/AuNPs	935	3.71×10⁻³~8.5	1.22	0.1 mol/L NaOH	[32]
CuO/MoS₂	1055	0.1~10	−	0.1 mol/L NaOH	[33]
莠草状	1281	0.05~2.0	16.7	0.1 mol/L NaOH	[34]
CuO/Cu₂O纳米线	1281	0.05~2.0	16.7	0.1 mol/L NaOH	[34]
CuO/Cu₂O/SnO₂纳米棒	2043	0.05~2.0	16.7	0.1 mol/L NaOH	[27]
多孔泡沫状CuO	6.17	<1	65.3	0.1 mol/L NaOH	[35]
注：−表示参考文献中作者未标注线性范围、检出限，表示未检出明确的范围与检出限，表2、表3同。

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表 2 Co₃O₄基非酶促葡萄糖传感器性能

Table 2. Co₃O₄-based non-enzymatic glucose sensor performance

电极材料	灵敏度（µA mmol⁻¹cm⁻²）	线性范围（mmol/L）	检出限（µmol/L）	缓冲溶液	参考文献
Co₃O₄微薄片	339.5	<0.22	—	0.1 mol/L KOH	[36]
Co₃O₄NPs-LIG	214	1×10⁻³~9	0.41	0.1 mol/L NaOH	[21]
针状Co₃O₄/柔性碳布	2591.6	2×10⁻³~1.0	0.23	0.1 mol/L NaOH	[37]
针状Co₃O₄/柔性碳布	271.2	1.0~10	0.23	0.1 mol/L NaOH	[37]
多孔Co₃O₄纳米板	212.92	0.05~3.2	2.7	0.1 mol/L NaOH	[38]
Co-Co₃O₄/CNT/CF	637.5	1.2×10⁻³~2.29	0.4	0.1 mol/L NaOH	[39]
Co₃O₄/Au	4470.4	2×10⁻³~2.11	0.085	0.1 mol/L NaOH	[40]
Au@Co₃O₄-S	1127.3	2×10⁻⁴~3.1	0.09	0.1 mol/L NaOH	[41]

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表 3 NiO基的非酶促葡萄糖传感器性能

Table 3. NiO-based non-enzymatic glucose sensor performance

电极材料	灵敏度（µA mmol⁻¹cm⁻²）	线性范围（mmol/L）	检出限（µmol/L）	缓冲溶液	参考文献
NiO纳米结构/镍板	206.9	0.1~10	1.16	0.1 mol/L KCl 0.5 mol/L NaOH	[42]
Ni/NiO/NG	3.2518	1×10⁻³~3.568	0.032	0.1 mol/L NaOH	[43]
Au@NiO	5536.2	1×10⁻³~6	0.25	0.1 mol/L NaOH	[44]
NiO/FTO	2632.53	5×10⁻³~0.825	0.084	0.1 mol/L NaOH	[45]
NiO-PPy/GCE	1094.8 62.9	0.01~0.5 1~20	5.8	0.1 mol/L PBS	[46]
NiO@CF	878.6	—	0.37	0.1 mol/L KOH	[47]
NiO/CC	5752	6×10⁻³~2	7.45	0.1 mol/L NaOH	[48]
NiO NC-rGO/GCE	4254	0.5~20	0.079	0.1 mol/L NaOH	[49]
NiO/碳多孔复合材料	2918.2	5×10⁻³~4.1	0.92	0.1 mol/L NaOH	[50]
NiO/PMB/GCE	413.06	3×10⁻³~0.8	2.1	0.1 mol/L NaOH	[51]
GLAD NiO	4400	5×10⁻⁴~9	—	0.1 mol/L NaOH	[52]

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