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中国精品科技期刊2020

响应面法优化蒲桃籽中三萜类化合物的提取工艺及其抗氧化活性分析

刘嘉鑫 陈小梅 曾慧 王淑美 向丽敏

刘嘉鑫,陈小梅,曾慧,等. 响应面法优化蒲桃籽中三萜类化合物的提取工艺及其抗氧化活性分析[J]. 食品工业科技,2022,43(23):192−199. doi:  10.13386/j.issn1002-0306.2022020016
引用本文: 刘嘉鑫,陈小梅,曾慧,等. 响应面法优化蒲桃籽中三萜类化合物的提取工艺及其抗氧化活性分析[J]. 食品工业科技,2022,43(23):192−199. doi:  10.13386/j.issn1002-0306.2022020016
LIU Jiaxin, CHEN Xiaomei, ZENG Hui, et al. Optimization of the Extraction Process of Triterpenoids from the Seeds of Syzygium jambos L. Alston by Response Surface Methodology and Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2022, 43(23): 192−199. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2022020016
Citation: LIU Jiaxin, CHEN Xiaomei, ZENG Hui, et al. Optimization of the Extraction Process of Triterpenoids from the Seeds of Syzygium jambos L. Alston by Response Surface Methodology and Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2022, 43(23): 192−199. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2022020016

响应面法优化蒲桃籽中三萜类化合物的提取工艺及其抗氧化活性分析

doi: 10.13386/j.issn1002-0306.2022020016
基金项目: 2020年广东大学生科技创新培育专项资金资助项目( pdjh2020b0306)。
详细信息
    作者简介:

    刘嘉鑫(1998−),女,硕士研究生,研究方向:中药化学成分的研究与应用,E-mail:1206358058@qq.com

    通讯作者:

    王淑美(1966−),女,博士,教授,研究方向:中药药效物质基础及质量控制研究,E-mail:2395903468@qq.com

    向丽敏(1988−),女,博士,副教授,研究方向:中药学,E-mail:xianglimin88@163.com

  • 中图分类号: TS201.1;R284.2

Optimization of the Extraction Process of Triterpenoids from the Seeds of Syzygium jambos L. Alston by Response Surface Methodology and Its Antioxidant Activity

  • 摘要: 以蒲桃籽为原料,通过单因素实验考察各因素对蒲桃籽中三萜类化合物得率的影响。运用Design-Expert 12软件设计响应面法优化提取工艺,并进行模型诊断和工艺验证。最后对提取得到的蒲桃籽三萜进行DPPH·、ABTS+·清除能力的测定,评价其抗氧化活性。结果表明,蒲桃籽三萜类化合物的最优提取工艺为甲醇体积分数44.30%、料液比1:47.18 g/mL、提取时间101.07 min(实验调整为101 min)。此条件下,实际得率(12.11 mg/g)与理论得率(12.28 mg/g)之间的偏差仅为1.26%(<5%),误差较小。蒲桃籽三萜类化合物DPPH·、ABTS+·清除能力的IC50值分别是为24.93、12.16 μg/mL,并且抗氧化活性与其浓度呈现出一定的量效关系。此优化实验有效可行,且蒲桃籽三萜类化合物具有较强的抗氧化活性。本文的研究为蒲桃资源的开发利用提供了一定的理论依据。
  • 图  1  甲醇体积分数对三萜类化合物得率的影响

    Figure  1.  Effect of methanol volume fraction on the yield of triterpenoids

    注:不同小写字母表示差异显著(P<0.05),图2~图4同。

    图  2  提取温度对三萜类化合物得率的影响

    Figure  2.  Effect of extraction temperature on the yield of triterpenoids

    图  3  提取时间对三萜类化合物得率的影响

    Figure  3.  Effect of extraction time on the yield of triterpenoids

    图  4  料液比对三萜类化合物得率的影响

    Figure  4.  Effect of material-liquid ratio on the yield of triterpenoids

    图  5  模型诊断工具图

    Figure  5.  Diagnostic tool diagram of the model

    注:(a)表示预测值内部t化残差分布;(b)表示内部t化残差正态分布概率。

    图  6  蒲桃籽总三萜、VC的DPPH·清除能力测定结果

    Figure  6.  Results of DPPH scavenging ability of total triterpenoids in the seeds of S. jambos L. Alston and VC

    注:不同小写字母表示蒲桃籽总三萜在不同浓度下抗氧化活性差异显著(P<0.05);不同大写字母表示VC在不同浓度下抗氧化活性差异显著(P<0.05);图7同。

    图  7  蒲桃籽总三萜、VC的ABTS+·清除能力测定结果

    Figure  7.  Results of ABTS+· scavenging ability of total triterpenoids in the seeds of S. jambos L. Alston and VC

    表  1  Box-Behnken试验设计因素和水平

    Table  1.   Test factors and levels of Box-Behnken design


    水平
    因素
    A
    甲醇体积分数(%)
    B
    提取时间(min)
    C
    料液比(g/mL)
    −130601:10
    050901:40
    1701201:70
    下载: 导出CSV

    表  2  Box-Behnken 试验设计和结果

    Table  2.   Box-Behnken experimental design and results

    实验号A甲醇体积分数B提取时间C料液比D得率(mg/g)
    100011.92
    2−1−1010.80
    310−17.74
    401−18.72
    50−1−19.00
    600012.36
    71−1011.20
    800011.81
    9−10110.95
    1000012.06
    1111010.89
    1200012.44
    1301110.29
    141019.62
    15−10−17.80
    160−1110.49
    17−11011.81
    下载: 导出CSV

    表  3  回归方程方差分析

    Table  3.   Analysis of variance of regression equation

    方差来源平方和自由度均方FP显著性
    模型36.078394.008726.68580.0001**
    A0.459910.45993.06150.1236
    B0.005810.00580.03850.8499
    C8.195018.195054.55370.0002**
    AB0.436410.43642.90510.1321
    AC0.406910.40692.70840.1438
    BC0.002210.00220.01460.9071
    2.500312.500316.64460.0047**
    0.122610.12260.81600.3964
    22.7097122.7097151.1779<0.0001**
    残差1.051570.1502
    失拟项0.754030.25133.37830.1351不显著
    纯误差0.297640.0744
    总离差0.371316
    注:**P<0.01表示差异极显著;*P<0.05表示差异显著。
    下载: 导出CSV
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  • 收稿日期:  2022-02-10
  • 网络出版日期:  2022-10-21
  • 刊出日期:  2022-11-23

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