Abstract: The effect of growth promoting agents on the flavor of netted melon was evaluated. During the planting, Bacillus subtilis, Trichoderma harzianum and Streptomyces were applied to the roots for 4 times at a dosage of 0.1 g/m² respectively. After harvest, volatile components of fruits were evaluated and compared with the untreated ones as the control based on the SPME-GC-MS and electric nose analysis. The results showed that the growth promoting agents affected the composition and concentration of volatile components in netted melon. A total of 54 volatile components were detected in Trichoderma harzianum group, whose content was 294.46 μg/kg being 22.66% higher than that of the control. A total of 13 aroma components was determined in Trichoderma harzianum group including trans-6-nonenal, cis-6-nonenol, octanal, trans-2-nonenal, trans,cis-2,6-nonenal, β-Violone, 3,6-nonyl-1-alcohol, nonanal, 1-octene-3-alcohol, heptanaldehyde, 2-methylbutyraldehyde, cis-3-hexenal and ethyl butyrate. The number and aroma activity value of the aroma components in the Trichoderma harzianum group was 33.33% and 82.53% higher than those of the control, respectively. Therefore, Trichoderma harzianum could improve the flavor richness and intensity of netted melon.