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中国精品科技期刊2020
杨静,李博,张延妮. 拐枣多糖的反复冻融回流工艺优化及抗氧化活性研究[J]. 食品工业科技,2022,43(19):273−279. doi: 10.13386/j.issn1002-0306.2022010018.
引用本文: 杨静,李博,张延妮. 拐枣多糖的反复冻融回流工艺优化及抗氧化活性研究[J]. 食品工业科技,2022,43(19):273−279. doi: 10.13386/j.issn1002-0306.2022010018.
YANG Jing, LI Bo, ZHANG Yanni. Optimization of Repeated Freezing-Thawing Reflux for Extraction of Polysaccharide from Hovenia dulcis and Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2022, 43(19): 273−279. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022010018.
Citation: YANG Jing, LI Bo, ZHANG Yanni. Optimization of Repeated Freezing-Thawing Reflux for Extraction of Polysaccharide from Hovenia dulcis and Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2022, 43(19): 273−279. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022010018.

拐枣多糖的反复冻融回流工艺优化及抗氧化活性研究

Optimization of Repeated Freezing-Thawing Reflux for Extraction of Polysaccharide from Hovenia dulcis and Its Antioxidant Activity

  • 摘要: 为了从拐枣中提取更多的多糖并提高其提取物的抗氧化活性,将反复冻融技术整合到回流提取方法中,通过正交试验优化了影响多糖含量的主要冻融条件,最后对所获多糖提取物的抗氧化活性进行了评价。结果显示,较佳的冻融条件为:溶胀加水量40 mL/100 g,冻结时间4 h,冻融次数2 次,解冻温度60 ℃;此时拐枣中多糖的含量为1.68%±0.03%;多糖提取物对DPPH自由基(提取物浓度为0.10 mg/mL)和ABTS自由基(提取物浓度为2.0 mg/mL)清除率可分别达到86.73%和91.33%。研究结果为拐枣多糖的综合利用提供了一定的参考。

     

    Abstract: In order to extract more polysaccharide from Hovenia dulcis and improve the antioxidant activity of the extract, repeated freezing-thawing technology coupled with reflux extraction method was employed, and major freezing-thawing conditions affecting the content of polysaccharide were optimized via orthogonal tests; In the end, the antioxidant activity of polysaccharide extract was evaluated. The results indicated that the optimal freezing-thawing conditions were as follows: The water content for swelling was set at 40 mL/100 g, freezing time was 4 h, the time of repetition of freezing-thawing was twice, and thawing temperature was 60 °C, under which the content of polysaccharides in Hovenia dulcis studied was 1.68%±0.03%. The clearance rates of polysaccharide extract to DPPH free radical and ABTS free radical were up to 86.73% (when the concentration of extract was 0.10 mg/mL) and 91.33% (when the concentration of extract was 2.0 mg/mL), respectively. The results provide a reference for the comprehensive utilization of polysaccharide from Hovenia dulcis.

     

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