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中国精品科技期刊2020
韩翔鹏,陈清莹,张杏果,等. 粪肠球菌Z096对副溶血弧菌生物被膜及群体感应的抑制作用[J]. 食品工业科技,2022,43(19):167−176. doi: 10.13386/j.issn1002-0306.2021120291.
引用本文: 韩翔鹏,陈清莹,张杏果,等. 粪肠球菌Z096对副溶血弧菌生物被膜及群体感应的抑制作用[J]. 食品工业科技,2022,43(19):167−176. doi: 10.13386/j.issn1002-0306.2021120291.
HAN Xiangpeng, CHEN Qingying, ZHANG Xingguo, et al. Inhibitory Effects of Enterococcus faecalis Z096 on Biofilm and Quorum Sensing of Vibrio parahaemolyticus[J]. Science and Technology of Food Industry, 2022, 43(19): 167−176. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021120291.
Citation: HAN Xiangpeng, CHEN Qingying, ZHANG Xingguo, et al. Inhibitory Effects of Enterococcus faecalis Z096 on Biofilm and Quorum Sensing of Vibrio parahaemolyticus[J]. Science and Technology of Food Industry, 2022, 43(19): 167−176. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021120291.

粪肠球菌Z096对副溶血弧菌生物被膜及群体感应的抑制作用

Inhibitory Effects of Enterococcus faecalis Z096 on Biofilm and Quorum Sensing of Vibrio parahaemolyticus

  • 摘要: 为了研究粪肠球菌Z096对副溶血弧菌生物被膜和群体感应(quorum sensing,QS)系统的抑制作用,采用竞争、清除、排阻三种方式模拟Z096与副溶血弧菌在微菌落环境中的相互作用,并进一步探究了Z096的提取物(Z096-E)对副溶血弧菌生物被膜形成、成熟生物被膜清除、细胞表面疏水性、自聚性、QS信号分子AI-2活性、群集泳动能力以及胞外多糖和蛋白合成的影响。结果表明:Z096可通过竞争、清除、排阻的方式与副溶血弧菌相互作用,降低浮游和生物被膜状态的副溶血弧菌细胞数量,干扰副溶血弧菌在载体表面的粘附,且Z096-E能够显著抑制副溶血弧菌生物被膜形成,有效清除成熟生物被膜,1.6 mg/mL的Z096-E处理12 h,副溶血弧菌生物被膜抑制率为70.43%,代谢活性减少率为84.15%;12.8 mg/mL的Z096-E处理副溶血弧菌成熟生物被膜4 h,生物被膜清除率为58.21%,代谢活性减少率为69.84%。而且1.6 mg/mL的Z096-E对副溶血弧菌群集和泳动能力、细胞表面疏水性和自聚性、胞外多糖和蛋白合成的抑制率分别为47.26%、53.56%、63.37%、89.38%、77.65%和51.91%,抑制效果具有浓度依赖性。此外,Z096-E可使副溶血弧菌QS信号分子AI-2活性减弱,表明Z096-E是一种AI-2类群体感应抑制剂,其可通过干扰QS系统,从而影响副溶血弧菌的生理特性。因此,本研究发现了一株能够抑制副溶血弧菌生物被膜的乳酸菌,其提取物Z096-E能作为一种防控副溶血弧菌生物被膜的新型乳酸菌生物制剂,这对消除致病菌生物被膜污染以及开发新型抗菌剂具有积极的作用。

     

    Abstract: To study the inhibitory effects of Enterococcus faecalis Z096 on the biofilm and quorum sensing (QS) system of Vibrio parahaemolyticus, the competition, elimination and exclusion were performed to simulate the interaction between Z096 and V. parahaemolyticus in microbial communities. In addition, the effects of Z096 extract (Z096-E) on V. parahaemolyticus biofilm formation, mature biofilm elimination, cell surface hydrophobicity, auto-aggregation, QS signal molecule AI-2 activity, motility ability (swarming and swimming), extracellular polysaccharide and protein synthesis were further explored. The results showed that Z096 could significantly reduce the number of V. parahaemolyticus cells in plankton and biofilm by competition, elimination and exclusion, and interfere with the adhesion of V. parahaemolyticus on the carrier surface. Moreover, Z096-E could significantly inhibit biofilm formation and effectively eliminate the mature biofilm of V. parahaemolyticus. When treated with 1.6 mg/mL of Z096-E for 12 h, the biofilm inhibition rate was 70.43%, and the metabolic activity decreased by 84.15%; When the mature biofilm of V. parahaemolyticus was treated with 12.8 mg/mL of Z096-E for 4 h, the biofilm removing rate was 58.21%, and the metabolic activity decreased by 69.84%. The swarming and swimming ability, cell surface hydrophobicity and auto-aggregation, extracellular polysaccharide and protein synthesis of V. parahaemolyticus were inhibited by 1.6 mg/mL of Z096-E by 47.26%, 53.56%, 63.37%, 89.38%, 77.65% and 51.91%, respectively, and the inhibitory effect was dose-dependent. In addition, Z096-E weakened the activity of QS signal molecule AI-2 of V. parahaemolyticus, indicating that Z096-E was an AI-2 quorum sensing inhibitor, which could affect the physiological characteristics of V. parahaemolyticus by interfering with the QS system. Therefore, we found one strain of lactic acid bacteria (LAB) that could inhibit V. parahaemolyticus biofilm, and the Z096-E could be used as a novel LAB-based biological agent to prevent and control V. parahaemolyticus biofilm. This study would be of positive significance to eliminate V. parahaemolyticus biofilm pollution and develop novel antibacterial agents.

     

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