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中国精品科技期刊2020

基于主成分分析和聚类分析的蓝莓品质综合评价

许文静, 陈昌琳, 邓莎, 刘怡君, 吕远平

许文静,陈昌琳,邓莎,等. 基于主成分分析和聚类分析的蓝莓品质综合评价[J]. 食品工业科技,2022,43(13):311−319. doi: 10.13386/j.issn1002-0306.2021110198.
引用本文: 许文静,陈昌琳,邓莎,等. 基于主成分分析和聚类分析的蓝莓品质综合评价[J]. 食品工业科技,2022,43(13):311−319. doi: 10.13386/j.issn1002-0306.2021110198.
XU Wenjing, CHEN Changlin, DENG Sha, et al. Comprehensive Evaluation of Blueberry Quality Based on Principal Component Analysis and Cluster Analysis[J]. Science and Technology of Food Industry, 2022, 43(13): 311−319. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021110198.
Citation: XU Wenjing, CHEN Changlin, DENG Sha, et al. Comprehensive Evaluation of Blueberry Quality Based on Principal Component Analysis and Cluster Analysis[J]. Science and Technology of Food Industry, 2022, 43(13): 311−319. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021110198.

基于主成分分析和聚类分析的蓝莓品质综合评价

基金项目: 四川省科技计划项目(重点研发项目2020YFN0149);四川省重点研发项目(省院省校科技合作),蓝莓冷链保鲜与精深加工技术研究(2022YFSY0031)
详细信息
    作者简介:

    许文静(1998−),女,硕士研究生,研究方向:健康食品工程,E-mail:1054243824@qq.com

    通讯作者:

    吕远平(1971−),女,博士,教授,研究方向:食品科学,E-mail:364943477@qq.com

  • 中图分类号: TS255

Comprehensive Evaluation of Blueberry Quality Based on Principal Component Analysis and Cluster Analysis

  • 摘要: 目的:探究蓝莓的综合品质特性。方法:利用全自动氨基酸分析仪、气相色谱-质谱联用仪等仪器,测定四川地区6个品种蓝莓的感官形态、营养功能、游离氨基酸、挥发性风味物质等20个指标,并结合主成分分析法及聚类分析评价其综合品质。结果:不同品种蓝莓各项指标间存在差异,布里吉塔的单果重(2.06 g)最重,横径(14.39 mm)最大,但胶粘性(151.96 N)和总酚含量(1.64 mg/g)最低;密斯梯的游离氨基酸含量最高(453.72 mg/100 g),鲜味氨基酸和甜味氨基酸占比较大;绿宝石的游离氨基酸含量最低(122.93 mg/100 g)且种类最少;园蓝的硬度适中,糖酸比(28.23)、花青素(2.09 mg/g)、总酚(5.78 mg/g)及挥发性风味物质(14.60 μg/g)含量最高。结论:根据主成分分析得到的最优品种为园蓝;根据聚类分析将蓝莓分为三类,适宜鲜食品种为园蓝和莱格西;加工鲜食皆宜品种为绿宝石、布里吉塔和密斯梯,蓝雨品种加工优势突出。
    Abstract: Objective: To explore the comprehensive quality characteristics of blueberries. Methods: 20 indexes including sensory morphology, nutritional function, free amino acids and volatile flavor substance content of six varieties of blueberries in Sichuan were detected by automatic amino acid analyzer and gas chromatography-mass spectrometry and other instruments. Furthermore, its comprehensive quality was evaluated by the principal component analysis and cluster analysis. Results: There were differences among different blueberry varieties. The single fruit weight (2.06 g) and transverse diameter (14.39 mm) of Brigita were the highest, while the gumminess (151.96 N) and total phenol content (1.64 mg/g) were the lowest; Mistry had the highest free amino acid content (453.72 mg/100 g), and the proportion of umami and sweet amino acids was higher; Emerald had the lowest free amino acid content (122.93 mg/100 g) and the least variety; Gardenblue had moderate hardness, its sugar-acid ratio (28.23), anthocyanin content (2.09 mg/g), total phenol content (5.78 mg/g) and flavor substances content (14.60 μg/g) were the highest. Conclusions: According to principal component analysis, the optimal variety was Gardenblue. Cluster analysis divided blueberries into three categories, suitable for fresh-eating varieties including Gardenblue and Legacy; suitable for both processing and fresh-eating varieties were Emerald, Brigitta and Misty; while Bluerain had outstanding processing advantages.
  • 蓝莓(Vaccinium spp.)又名越橘,是杜鹃花科越橘属植物,已被全球公认为是一种能促进健康的重要饮食来源,近年来其产量和消费量均大幅增加[1]。蓝莓营养丰富,富含花青素、多酚、黄酮等天然抗氧化物,可用于对抗某些人类癌症、调节血糖水平、降低器官氧化应激、调节肠道菌群、调节炎症反应以及改善儿童认知表现等[2-4]

    随着蓝莓产量的增加,不同品种蓝莓的形态特征、质地组成和营养特性各不相同,导致种植者难以科学地选择栽培品种;同时消费者对蓝莓的要求更加具体,表现在外观品质(果实大小、色泽、质构)、香气滋味(糖、酸、挥发性风味物质)及营养功能(花青素、总酚)上。目前,国内外对蓝莓品质的评价研究多集中在营养指标、抗氧化能力分析上,对品种间差异及加工适应性的研究稍有不足。如刘笑宏等[5]对胶东地区4个品种蓝莓的11个理化指标及芳香物质进行分析,但缺少质构及色差等指标分析;温靖等[6-8]探讨了7个蓝莓品种的品质特性,充分分析了其感官品质、理化指标及抗氧化能力,但欠缺对蓝莓氨基酸组成的分析。

    目前对山东、广东等地区的引种蓝莓研究较多,因此,本文测定了四川地区6个蓝莓品种的感官形态、色泽质构、营养功能指标、氨基酸组成及风味物质含量等指标,全面评价蓝莓的综合品质,同时利用主成分分析[9]和聚类分析[10]等方法进行比较分类,能客观有效的反映品种间差异,对品种选育、市场推广和采后加工具有生产指导意义。

    供试6个蓝莓品种(莱格西、绿宝石、密斯梯、蓝雨、布里吉塔、园蓝) 四川省农业科学院经济作物育种栽培研究所。经原料验收,果实大小一致、无病、虫害,部分贮藏于4 ℃用于感官形态品质指标及水分含量测定,部分置于−20 ℃贮藏备用;DNS(二硝基水杨酸) 厦门海标科技有限公司;2-辛醇 上海麦克林生化科技有限公司;没食子酸、芦丁、福林酚、盐酸、硫酸、氯化钾 成都市科隆化学品有限公司;以上试剂均为分析纯。

    CM-5型色度色差仪 日本柯尼卡美能达;TA-XT plus型质构仪 英国SMS;A300全自动氨基酸分析仪 德国曼默博尔;QP2010SE气相色谱质谱联用仪 日本岛津。

    单果重:每个品种随机选取30个果实,称重后计算单果重,重复测定3次[6];果形指数:每个品种随机选取20个果实用游标卡尺测量横径与纵径,重复测定3次,果形指数=纵径/横径[7];色度:采用色度色差仪测定不同品种蓝莓L*、a*、b*值,平行测12次;质构:采用TPA(全质构测试)分析,测定时样品果柄处向上,平行测定15次。使用直径75 mm(P36R)圆形探头,仪器参数设置为测前速度0.5 mm/s,测试速度1 mm/s,测后速度0.5 mm/s,压缩程度30%,2次压缩停顿时间为5 s,触发力5 g[11]

    水分含量:采用GB 5009.3-2016《食品中水分的测定》第一法直接干燥法测定;可溶性固形物(total soluble solid,TSS)含量:参照NY/T 2637-2014《水果和蔬菜可溶性固形物含量的测定 折射仪法》测定;总酸含量:采用GB 12456-2021《食品中总酸的测定》第二法pH计电位滴定法测定;还原糖含量:依照GB 5009.7-2016《食品中还原糖的测定》进行测定;可溶性糖含量:采用NY/T 2742-2015《水果及制品可溶性糖的测定3,5-二硝基水杨酸比色法》测定。

    花青素含量:采用pH示差法[12-13]测定:即1 g蓝莓研磨后加入12 mL 80%甲醇振荡提取20 min后6000 r/min离心15 min得提取液。分别取1 mL提取液于2支25 mL具塞试管中,分别用pH为1.0和4.5的缓冲溶液定容至10 mL,在40 ℃水浴锅中平衡50 min后测定535、700 nm吸光度值计算花青素含量,以矢车菊素-3-O-葡萄糖苷计。

    总酚含量:采用Folin-Ciocalteu法[14]测定,即1 g蓝莓加入4 mL 70%乙醇研磨后转入离心管中70 ℃水浴提取10 min,4200 r/min离心15 min后收集上清液至10 mL容量瓶,残渣反复提取至刻度。取1 mL提取液于10 mL棕色容量瓶中,分别加入1 mL福林酚试剂、3 mL 7.5% Na2CO3后定容,避光显色1 h后测定760 nm吸光度值,以没食子酸为标准品,结果以没食子酸当量表示(mg/g)。

    黄酮含量:参考邵盈盈[15]的方法进行测定,以芦丁为标准品,结果以芦丁当量表示(mg/g)。

    采用氨基酸自动分析仪测定游离氨基酸含量,样品前处理依据GB 30987-2020《植物中游离氨基酸的测定》进行,分离柱为阳离子交换树脂2619(4.6 mm×60 mm,3 μm),柱温57 ℃,反应柱温度135 ℃,检测波长570、440 nm,缓冲溶液流速0.40 mL/min,茚三酮流速0.35 mL/min,进样量20 μL[16]

    采用顶空固相微萃取和气相色谱-质谱联用仪对6个品种蓝莓进行香气检测与分析。根据Ke等[17]的处理方法并加以改动:称取研磨后的蓝莓5 g于20 mL顶空瓶中,加入0.1 g NaCl与15 μL内标物质(40 mg/L 2-辛醇)后密封。样品先在40 ℃下平衡20 min,再将填充有65 μm PDMS/DVB材料的萃取头插入顶空瓶中萃取30 min,最后插入GC进样口,解析3 min,以2-辛醇为内标对风味物质进行定量分析。

    GC条件:参照吴林等[18]的方法稍作修改,色谱柱DB-5MS(30 m×250 μm,0.25 μm),进样口温度250 ℃,色谱柱起始温度50 ℃,升温程序为以50 ℃保持2 min,以5 ℃/min升至100 ℃保持5 min;8 ℃/min 升至180 ℃保持4 min;15 ℃升至230 ℃保留2 min。载气为高纯He,流量1.0 mL/min。MS条件:离子源温度200 ℃,接口温度250 ℃;离子源:EI;电子能量:70 eV;扫描范围:35~625 amu。

    所有数据以平均值±标准差表示,采用SPSS 26软件对数据进行Duncan’s差异显著性分析,P<0.05时显著;通过SPSS 26软件进行主成分分析、聚类分析;采用Origin 2018软件作图。

    表1可知,蓝莓果实单果重为0.85~2.06 g,高于2.00 g的有莱格西和布里吉塔,果实较重;单果质量最轻的为蓝雨,仅为0.85 g,且各品种间差异显著(P<0.05)。判定果实形状的直观标准是果形指数,通常果形指数在0.6~0.8为扁圆形,0.8~0.9为圆形,0.9~1.0为椭圆形或圆锥形,1.0以上为长圆形[19]。蓝雨果实小且呈椭圆形,园蓝则为圆形代表,其余品种皆为扁圆形,这主要受蓝莓遗传基因的影响。由单果质量和横、纵径可判断出布里吉塔和莱格西属于大果型蓝莓,密斯梯、蓝雨和园蓝为中小果形蓝莓。

    表  1  不同品种蓝莓形态指标比较
    Table  1.  Comparison of morphological indexes of different varieties of blueberries
    品种单果质量(g)横径(mm)纵径(mm)果形指数果实形状
    莱格西2.01±0.03a13.44±0.77b10.25±0.59b0.76±0.04c扁圆形
    绿宝石1.86±0.09b13.00±0.86c9.86±0.75b0.76±0.05c扁圆形
    密斯梯1.35±0.06c11.21±0.77d8.13±0.60d0.72±0.03d扁圆形
    蓝雨0.85±0.02e9.69±0.52e8.90±0.44c0.92±0.04a椭圆形
    布里吉塔2.06±0.07a14.39±0.50a11.32±0.90a0.79±0.05c扁圆形
    园蓝1.09±0.08d10.85±0.51d8.99±0.63c0.83±0.05b圆形
    注:同列不同字母表示差异显著(P<0.05),表2~表5同。
    下载: 导出CSV 
    | 显示表格

    颜色是食品重要的外观指标之一,色差仪中L*值表示样品的明暗度,a*值表示红绿度,b*值表示黄蓝度[20]。不同品种蓝莓色度参数见表2,莱格西、绿宝石L*值较高,说明这两个品种果皮明亮度高,与其余品种差异显著(P<0.05)。a*值密斯梯最大(1.74),b*值绿宝石最小(−6.10)。所有品种蓝莓a*值均大于0,说明果皮颜色偏红,b*值均小于0,表明蓝莓颜色偏蓝,整体呈现明亮的蓝紫色。

    表  2  不同品种蓝莓色度比较
    Table  2.  Comparison of chroma of different blueberries
    品种L*a*b*
    莱格西41.74±4.6a1.18±0.11bc-6.09±1.42b
    绿宝石42.86±3.46a1.49±0.51ab-6.10±1.17b
    密斯梯37.07±4.03b1.74±0.10a-5.99±0.95b
    蓝雨28.75±2.52c1.02±0.25c-4.84±0.75a
    布里吉塔25.30±1.98d1.43±0.95ab-4.63±1.36a
    园蓝35.13±2.79b0.65±0.06d-5.19±0.84ab
    下载: 导出CSV 
    | 显示表格
    表  4  不同品种蓝莓的水分含量、TSS、还原糖、可溶性糖、总酸及糖酸比
    Table  4.  Water content, TSS, reducing sugar, soluble sugar, total acid and sugar-acid ratio of different blueberries
    品种水分含量(%)TSS(%)还原糖(%)可溶性糖(%)总酸(%)糖酸比
    莱格西85.96±0.16bc10.7±0.12d9.66±1.49ab10.63±0.77b1.02±0.1b10.48±1.38bc
    绿宝石88.11±0.83a11.3±0.23c7.81±0.45cd11.04±1.26ab1.14±0.01a9.66±2.87c
    密斯梯86.53±0.51b11.9±0.12b9.99±0.07a11.70±1.84ab1.00±0.10b11.74±1.89b
    蓝雨85.28±0.15c10.5±0.12e7.37±0.32d8.93±0.07c1.18±0.03a7.59±0.18d
    布里吉塔87.72±0.35a11.1±0.12c8.38±0.87bcd10.51±0.40bc1.07±0.01ab9.86±0.41bc
    园蓝80.83±0.53d14.9±0.12a8.99±0.42abc12.61±0.48a0.45±0.02c28.23±0.46a
    下载: 导出CSV 
    | 显示表格

    表3可知,不同品种蓝莓硬度在408.37~805.67 g范围内,莱格西与绿宝石硬度相当,与其余品种间差异显著(P<0.05)。弹性代表蓝莓受到挤压后在一段时间内恢复原形的能力[21],可以理解为蓝莓在第一次咬合后与第二次咬合间恢复的高度。蓝莓的弹性除绿宝石外,其余品种差异不大;蓝雨和莱格西的胶粘性最大,分别为296.48 N和280.33 N,最小的为布里吉塔(151.96 N);咀嚼性反映蓝莓果实对咀嚼的持续抵抗力,蓝雨的咀嚼性最大,咀嚼韧性好,脆而不烂,其次为莱格西,最小为布里吉塔。

    表  3  不同品种蓝莓质构比较
    Table  3.  Texture comparison of different blueberry varieties
    品种硬度(g)弹性(mm)胶粘性(N)咀嚼性(mJ)
    莱格西715.31±90.61b0.69±0.03a280.33±41.8a183.67±26.75a
    绿宝石741.75±120.21b0.63±0.07b246.59±36.6b162.55±24.19b
    密斯梯439.41±80.36d0.69±0.03a170.33±15.88d114.44±12.06c
    蓝雨805.67±68.21a0.65±0.02ab296.48±24.82a193.86±19.31a
    布里吉塔408.37±58.17d0.66±0.06ab151.96±20.40d102.58±13.49c
    园蓝510.65±55.66c0.69±0.09a225.74±20.53c155.33±19.93b
    下载: 导出CSV 
    | 显示表格

    表4可知,6个品种蓝莓水分含量为80.83%~88.11%。园蓝的TSS、可溶性糖含量最高;最低的为蓝雨,其TSS和可溶性糖含量仅有园蓝的70%和71%。园蓝的总酸含量最低(0.45%),布里吉塔、密斯梯、莱格西之间差异不显著(P>0.05)。园蓝的糖酸比最高(28.23),莱格西、绿宝石、布里吉塔糖酸比接近,蓝雨果实糖酸比最低。从水分含量、可溶性糖和总酸含量来看,初步认为除蓝雨外其余品种高糖低酸、高水分含量[22],都可以作为鲜食品种销售。

    表5可知,园蓝和蓝雨的花青素含量较高,分别为2.09 mg/g、1.36 mg/g,与其余品种蓝莓差异显著(P<0.05),这与园蓝和蓝雨相对较厚的果皮有关,果皮中含有大量的花青素。园蓝的总酚和黄酮含量最高;总酚最低的为布里吉塔,仅有园蓝的28%,黄酮含量最少的为蓝雨仅有0.33 mg/g。蓝雨果实较小,糖酸比较低,鲜食销售优势不大,但功能成分花青素、总酚含量可观,更适宜加工。

    表  5  不同品种蓝莓花青素、总酚、黄酮含量比较
    Table  5.  Comparison of anthocyanins, total phenols and flavonoids contents in different blueberries
    品种花青素(mg/g)总酚(mg/g)黄酮(mg/g)
    莱格西0.84±0.23c2.35±0.2c0.68±0.06b
    绿宝石0.97±0.18c2.54±0.25c0.58±0.02b
    密斯梯1.05±0.17c3.07±0.13b0.65±0.01b
    蓝雨1.36±0.11b3.01±0.07b0.33±0.03d
    布里吉塔0.92±0.14c1.64±0.04d0.45±0.04c
    园蓝2.09±0.10a5.78±0.39a0.99±0.13a
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    不同品种蓝莓游离氨基酸组成及含量见表6。6个品种蓝莓共检测出18种游离氨基酸,其中,包含苏氨酸、缬氨酸、异亮氨酸、苯丙氨酸、亮氨酸、赖氨酸6种人体必需氨基酸,缺乏甲硫氨酸与色氨酸。不同蓝莓品种氨基酸组成相似,但含量差异较大,这与严红光等[23]的研究结果相似。游离氨基酸总量含量最多的为密斯梯(435.720 mg/100 g),布里吉塔和绿宝石游离氨基酸总量低于200 mg/100 g,仅有密斯梯的46%和28%。检测出的18种氨基酸中含量比较高的有Glu、Ala、GABA、Lys、Arg、Pro,含量较低的为Gly、Ile、Phe、Leu。有研究指出氨基酸的生物合成途径在果蔬和食用菌中完全不同,且其代谢途径的转录表达调控水平导致了代谢系统的稳定差异[24],不同蓝莓品种氨基酸含量差异可能与其遗传差异、氨基酸代谢途径中酶活力差异相关。

    表  6  不同品种蓝莓游离氨基酸组成
    Table  6.  Free amino acid composition of different blueberries
    游离氨基酸
    (mg/100 g)
    莱格西绿宝石密斯梯蓝雨布里吉塔园蓝
    天冬氨酸Asp8.128±0.78b8.319±0.63b10.989±0.71a8.767±1.34ab7.240±0.84b9.128±0.77ab
    苏氨酸Thr1.690±0.62b1.971±0.78ab3.103±0.70a2.108±0.71ab1.84±0.57b2.688±0.65a
    丝氨酸Ser5.019±0.72a6.735±1.40a5.882±1.41a6.025±1.40a6.1±1.48a5.382±0.64a
    天冬酰胺Asn1.752±0.70c4.115±0.74b29.074±1.40a4.843±1.39b4.135±1.40b4.681±2.14b
    谷氨酸Glu10.556±0.70c16.072±1.41b28.187±0.71a14.889±2.11b14.339±1.42b30.914±1.35a
    甘氨酸Gly0.443±0.44b0.353±0.21c0.576±0.35a
    丙氨酸Ala12.722±1.41a12.560±2.11a11.155±0.08a11.797±1.41a12.566±1.34a7.364±0.91b
    缬氨酸Val3.710±1.41b3.071±0.64c4.058±1.49a2.825±0.85d
    异亮氨酸Ile0.730±0.27a0.717±0.33a
    亮氨酸Leu0.772±0.28a0.760±0.26a
    γ-氨基丁酸GABA27.944±0.28a28.626±2.12a17.028±2.11d24.561±1.38ab19.897±1.56cd22.158±0.77bc
    组氨酸His1.761±0.82b2.675±2.12ab5.030±1.43a1.549±0.76b0.929±0.05b0.639±0.50b
    赖氨酸Lys36.874±1.41b38.052±1.41a42.145±2.05a30.590±1.40c19.015±0.64d5.970±1.45e
    精氨酸Arg60.693±0.79c212.721±3.54a51.791±2.13d24.516±1.34e89.471±1.28b
    脯氨酸Pro73.090±1.41b66.349±1.42c66.575±1.43c83.612±1.40a45.865±2.22d
    苯丙氨酸Phe0.737±0.27
    胱氨酸(Cys)23.810±1.40a1.995±0.84c3.450±0.73b
    酪氨酸Tyr7.876±1.41
    必需氨基酸占比(%)17.800±0.21b35.650±0.40a11.320±0.33d14.360±0.30c11.900±0.20d4.260±0.03e
    游离氨基酸总量245.880±4.13b122.930±3.21f435.720±4.33a227.660±5.16d199.010±2.18e237.640±3.25c
    注:同行不同小写字母表示差异显著(P<0.05),表7同。
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    根据游离氨基酸呈味的特点,参考侯娜等[25]和刘伟等[26]的分类并稍作修改,将所检测出的氨基酸分为5类:鲜味氨基酸(Asp、Glu、Gly、Ala、Lys)、甜味氨基酸(Ser、Thr、His、Pro)、芳香族氨基酸(Tyr、Phe)、苦味氨基酸(Arg、Val、Leu、Ile)和其他(Asn、GABA、(Cys)2)。由图1可知,不同品种蓝莓呈味氨基酸差异较大:相较于其他呈味氨基酸,蓝雨和园蓝的苦味氨基酸含量丰富;绿宝石和密斯梯的甜味氨基酸含量较多;布里吉塔所含鲜味、甜味、苦味氨基酸含量基本持平;莱格西则含有较多的鲜味氨基酸。

    图  1  不同蓝莓游离氨基酸滋味特征
    Figure  1.  Flavor characteristics of free amino acids in different blueberries

    不同品种蓝莓的挥发性风味成分见表7。6个品种蓝莓共检测73种挥发性风味成分,其中,园蓝、绿宝石分别检测出36、35种香气成分,莱格西和蓝雨检测出31种香气成分,布里吉塔和密斯梯分别检测出23、26种香气成分,不同品种蓝莓香气成分的类别和含量差异较大。挥发性风味成分总量及种类最多的为园蓝(14604.72 ng/g),其次为绿宝石(7052.68 ng/g),莱格西香气成分总量最低,仅有园蓝的11%。

    表  7  不同品种蓝莓挥发性风味物质组成(ng/g)
    Table  7.  Composition of volatile flavor compounds in different varieties of blueberries (ng/g)
    类别CAS号化合物品种
    布里
    吉塔
    莱格西蓝雨绿宝石密斯梯园蓝
    醇类470-82-6桉叶油醇593.93±9.59
    78-70-6芳樟醇1123.29±10.13c124.92±4.10f629.74±7.81e1454.75±12.10b949.27±9.12d6621.32±15.21a
    98-55-5alpha-松油醇31.58±1.02d278.88±3.34b201.95±1.23c581.89±4.66a
    7341-17-52-乙基己基硫醇159.65±1.02b203.29±3.41a52.56±0.46d61.77±0.82c
    97-99-4四氢糠醇34.27±0.11
    18409-18-2反式-2-癸烯醇52.04±0.13
    536-59-4紫苏醇26.17±0.09
    7786-44-92,6-壬二烯醇666.71±10.21b1329.42±13.20a
    5921-73-32-壬炔-1-醇1257.75±16.21
    34995-77-2三甲基-5-乙烯基四氢化-2-呋喃甲醇210.22±2.46
    74962-98-4反-2-十三烯醇32.22±0.21
    112-53-8十二醇22.85±0.34
    醛类100-52-7苯甲醛179.07±1.23b63.25±0.43c172.37±1.66b280.74±3.25a
    2548-87-0反-2-辛烯醛52.37±0.45
    124-19-6壬醛216.30±2.71a205.13±1.96b206.29±3.77b
    557-48-2反,顺-2,6-壬二烯醛21.73±0.70
    18829-56-6反式-2-壬烯醛56.55±0.45c116.21±1.21a73.58±0.48b
    112-31-2癸醛90.85±1.02f150.79±2.70d119.19±0.98e223.01±4.21b169.68±2.33c269.42±3.22a
    60784-31-8反-2-壬烯醛44.79±0.22c94.67±1.09a78.86±0.37b42.15±0.34c
    124-13-0正辛醛101.10±0.77
    112-54-9十二醛44.64±0.34
    141-27-5顺-3,7-二甲基-2,6-辛二烯醛49.70±0.36b80.60±0.93a
    萜烯类123-35-3月桂烯476.75±10.30d521.18±9.89b493.36±12.30c570.75±10.99a
    13877-91-3罗勒烯94.17±0.46e264.15±6.44c214.21±4.81d277.84±3.77b279.19±2.35b558.63±6.70a
    4316-65-83,5,5-三甲基-1-己烯58.74±0.53
    7785-26-4左旋-alpha-蒎烯218.58±2.21a168.00±1.70b
    586-62-9萜品油烯61.54±0.22b61.26±0.19b55.37±0.09c61.63±0.16b144.04±1.33a
    5392-40-5柠檬醛147.88±1.33
    87-44-51-石竹烯382.21±3.66a39.75±1.22c50.23±0.44b
    6753-98-6α-石竹烯78.78±0.78
    3338-55-4反-3,7-二甲基-1,3,6-十八烷三烯47.88±1.02
    1139-30-6氧化石竹烯27.75±0.50a13.41±0.06b
    5989-54-8(-)-柠檬烯48.98±0.80b1005.06±17.99a
    11028-42-5雪松烯77.59±1.55b160.00±2.52a
    489-39-4(+)-香橙烯26.74±0.39b92.31±0.81a25.34±0.08b
    5989-27-5右旋萜二烯874.75±10.02
    138-86-3双戊烯413.40±5.11
    29548-02-5黏蒿三烯197.79±1.44
    1195-92-2(R)-氧化柠檬烯58.25±0.62
    6931-54-0beta-环氧蒎烷41.04±0.09
    77-53-2柏木脑33.44±0.07
    2437-56-11-十三烯9.16±0.41
    酯类13361-34-7氰乙酸异辛酯29.56±0.11b42.56±0.41a
    103-11-7丙烯酸2-乙基己酯59.52±0.72e195.99±1.01c258.17±3.07b208.77±2.27c122.60±1.44d288.69±2.93a
    6846-50-02,2,4-三甲基-1,3-戊二醇二异丁酸酯61.89±0.60f123.61±1.02d262.23±2.33a179.46±0.88c205.99±1.63b109.15±1.00e
    84-69-5邻苯二甲酸二异丁酯67.91±0.17a11.73±0.03b
    23853-33-0硫代苯甲酸S-羟甲酯25.91±0.28b57.79±0.51a
    25415-84-32-乙基己酸乙酯85.05±0.72a64.54±0.51c71.76±0.40b28.34±0.05e56.80±0.34d
    141-12-8乙酸橙花酯21.13±0.02
    2566-89-4花生四烯酸甲酯46.21±0.15a24.76±0.04b30.73±0.21c
    1731-88-0十三碳酸脂肪酸43.34±0.11a42.26±0.17a34.10±0.12b
    111-82-0月桂酸甲酯90.66±1.03b43.71±0.18c113.50±0.77a
    110-42-9癸酸甲酯33.28±0.24b16.57±0.04c83.95±0.60a
    110-38-3癸酸乙酯76.25±0.35
    115-99-13,7-二甲基-1,6-辛二烯-3-醇甲酸酯57.20±0.15
    酮类112-12-9甲基壬基甲酮127.08±1.22d387.23±2.88b231.92±1.55c238.54±3.03c475.42±3.50a47.26±0.22e
    3796-70-1香叶基丙酮160.82±1.33b56.58±0.51d77.71±0.40c175.14±0.91a51.88±0.49e
    821-55-62-壬酮235.09±2.11a80.24±0.71c133.05±0.34b53.72±0.23d
    464-48-2左旋樟脑123.43±2.03
    2345-27-92-十四酮90.45±0.30
    6485-40-1左旋香芹酮145.00±1.39
    其他95-47-6邻二甲苯198.51±1.08
    629-20-9环辛四烯710.75±9.22c647.15±4.70d860.01±10.33b332.56±4.12e325.96±2.09f1024.07±15.23a
    629-50-5正十三烷17.92±0.03e182.47±0.40b61.31±0.12c40.51±0.06d215.79±1.13a
    544-76-3十六烷18.72±0.03d19.97±0.01d47.85±0.20b26.34±0.33c116.96±0.73a
    112-40-3十二烷18.39±0.02f81.07±0.53c113.16±0.77b33.26±0.11e43.38±0.21d164.23±1.00a
    1120-21-4十一烷39.11±0.06b34.58±0.03b74.61±0.12a37.30±0.01b
    119-36-8水杨酸甲酯142.91±1.02c160.12±1.34b287.87±2.11a
    15869-93-93,5-二甲基辛烷44.19±0.40
    124-18-5癸烷71.63±0.40
    5333-84-63-甲基四氢苯酐416.90±6.11a365.93±4.03b
    1138-52-93,5-二叔丁基苯酚28.89±0.02
    629-59-4十四烷40.07±0.05
    总量4466.41±16.42e1618.06±12.02f4942.14±18.29d7052.68±23.01b5800.32±20.44c14604.72±17.96a
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    结合表7图2可见,6个品种蓝莓共检测出12种醇类物质,园蓝中醇类物质最为丰富,主要含有芳樟醇、2,6-壬二烯醇、alpha-松油醇等物质,赋予蓝莓果香气息的同时表现出玫瑰木香气又似甜瓜香气。布里吉塔果实独有桉叶油醇,具有清香的植物香气[27]。在检测出的10种醛类物质里,布里吉塔和绿宝石中醛类物质含量较高,主要含有苯甲醛、反-2-辛烯醛、壬醛、反,顺-2,6-壬二烯醛和癸醛等化合物,呈现苦杏仁气味及绿叶清香,6个品种蓝莓均含有癸醛,表现出柑橘清样香气。绿宝石、密斯梯中萜烯类化合物较多,主要含有罗勒烯、月桂烯、萜品油烯、1-石竹烯等。萜类化合物广泛存在于浆果果实中,由于其具有较高的沸点而表现出较稳定的香气[28]。莱格西和园蓝酯类物质种类较多(8种),园蓝、蓝雨和绿宝石的酯类含量较高,主要含有月桂酸甲酯、丙烯酸2-乙基己酯、2,2,4-三甲基-1,3-戊二醇二异丁酸酯和癸酸甲酯等香气成分,使得蓝莓具有典型的果香。除甲基壬基甲酮、香叶基丙酮外,莱格西、蓝雨、绿宝石和密斯梯还含有2-壬酮,在蓝莓果香和植物香的基础上增添了甜香和青香。

    图  2  蓝莓香气成分比例
    Figure  2.  Proportion of aroma components in blueberry

    对6个品种蓝莓感官品质、游离氨基酸及挥发性风味化合物含量等20个指标进行主成分分析,结果如表8所示。提取出3个主成分,累计贡献率达89.223%,能解释绝大部分变量信息。主成分载荷矩阵可以表达出变量对相应主成分的影响程度[29]。由表9可知,对第一主成分产生正向影响的为糖酸比、TSS、花青素、总酚、黄酮含量,具有较高的载荷权数,产生负向影响的为总酸。第二主成分主要代表果形指数、游离氨基酸含量、胶粘性和咀嚼性,为正相关。第三主成分反映蓝莓色泽,与L*值呈正相关,b*值呈负相关。将三个主成分的得分记为F1、F2、F3,以3组主成分相应的特征值除以特征值总和作为权重数,建立主成分综合得分模型:F=0.50×F1+0.31×F2+0.19×F3,得分情况见表10。6种蓝莓的综合评分由高到低依次为:园蓝、蓝雨、莱格西、绿宝石、密斯梯、布里吉塔。

    表  8  主成分特征值及累计贡献率
    Table  8.  Principal component characteristic values and cumulative contribution rate
    主成分特征值贡献率(%)累计贡献率(%)
    18.83444.16944.169
    25.60628.02972.198
    33.40517.02589.223
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    表  9  主成分载荷表
    Table  9.  Principal component load table
    指标成分
    123
    单果重(X1−0.443−0.6530.122
    果形指数(X20.0590.939−0.292
    L*(X30.119−0.2770.910
    a*(X4−0.634−0.633−0.019
    b*(X5−0.209−0.051−0.887
    硬度(X6−0.3480.6920.620
    弹性(X70.583−0.4050.119
    胶粘性(X8−0.090.7340.65
    咀嚼性(X9−0.0250.7430.644
    水分含量(X10−0.907−0.35−0.005
    TSS(X110.972−0.072−0.125
    还原糖(X120.361−0.7410.279
    可溶性糖(X130.781−0.5620.083
    总酸(X14−0.9830.0690.059
    糖酸比(X150.986−0.012−0.064
    花青素(X160.8730.445−0.181
    总酚(X170.9480.2710.055
    黄酮(X180.897−0.3050.287
    游离氨基酸总量(X19−0.1670.833−0.438
    风味化合物总量(X200.8740.166−0.190
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    表  10  不同品种蓝莓主成分评分和综合评分
    Table  10.  Principal component score and comprehensive score of different blueberries
    品种F1F2F3F排名
    园蓝5.8460.755−0.3713.0601
    蓝雨−1.7524.215−0.4830.3652
    莱格西−0.863−0.8722.499−0.2243
    绿宝石−1.585−0.0091.325−0.5354
    密斯梯0.252−2.580−0.029−0.6925
    布里吉塔−1.898−1.508−2.941−1.9746
    下载: 导出CSV 
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    采用聚类分析法对20个品质指标进行R型聚类,对蓝莓6个品种进行Q型聚类,结果见图3图4。由图3可知,当距离为20时,可将蓝莓的品质指标分为三大类:第1类为氨基酸含量、果形指数、硬度、咀嚼性、胶粘性等指标;第2类指标为糖酸比、黄酮、花青素、总酚、TSS、风味化合物含量、弹性、还原糖、总糖、L*值;第3类指标为水分含量、总酸、a*、b*及单果重。结合主成分载荷表9可知,第2类指标对应第1主成分的正载荷量,即指标值与综合模型评分呈正相关。结合相关文献[30-31],确定蓝莓品质的核心指标包括糖酸比、黄酮、花青素、硬度、咀嚼性、胶粘性等。

    图  3  不同品种蓝莓R型聚类谱系图
    Figure  3.  R-type cluster pedigree diagram of different blueberry varieties
    图  4  不同品种蓝莓Q型聚类谱系图
    Figure  4.  Q-type cluster pedigree diagram of different blueberry varieties

    图4为不同品种蓝莓Q型聚类谱系图,可以将蓝莓分为三类。第一聚类包括蓝雨、布里吉塔、密斯梯、绿宝石,这四个品种水分含量丰富,可溶性固形物含量较高,总酸含量中等,同时花青素、总酚含量较高,属于鲜食加工皆可的品种。其中,蓝雨果实较小,商品性差,但高花青素含量使其更适宜被作为提取色素的原料利用。第二聚类为莱格西,其果实为扁圆形,属于中大型果实;高糖酸比、高硬度,是适宜鲜食和贮藏的蓝莓品种。第三聚类为园蓝,果实呈圆形,硬度适中,高糖酸比,抗氧化物质含量最高,鲜食的优势突出。

    科学的选择栽培品种对于果农来说至关重要,同时加工品种的选择影响产品深加工的品质;消费者也可以根据自身需求选择喜好的蓝莓品种。6个品种蓝莓在单果重、糖酸比、花青素和总酚含量方面差异显著,布里吉塔果实较大,但糖酸比较低,园蓝和蓝雨花青素、总酚含量较高。主成分分析综合得分排名前三的品种依次为园蓝、蓝雨、莱格西,结合聚类分析筛选出蓝莓品质核心指标为:糖酸比、黄酮、花青素、硬度、咀嚼性、胶粘性。根据Q型聚类分析可以将品种归为三类,第一聚类包括蓝雨、布里吉塔、密斯梯、绿宝石,属于鲜食加工皆可的品种,但蓝雨果实小且果皮厚,商品性差,但花青素含量高,可以作为提取色素原料利用或加工高花青素产品,更适合加工利用。第二聚类为莱格西,适宜鲜食。第三聚类为园蓝,适宜鲜食;其高糖酸比,高营养功能物质含量,更受消费者喜爱,可以进一步扩大其种植面积。本研究筛选出适宜加工的蓝莓品种,后续可以对蓝莓深加工及其花青素提取利用深入研究。

  • 图  1   不同蓝莓游离氨基酸滋味特征

    Figure  1.   Flavor characteristics of free amino acids in different blueberries

    图  2   蓝莓香气成分比例

    Figure  2.   Proportion of aroma components in blueberry

    图  3   不同品种蓝莓R型聚类谱系图

    Figure  3.   R-type cluster pedigree diagram of different blueberry varieties

    图  4   不同品种蓝莓Q型聚类谱系图

    Figure  4.   Q-type cluster pedigree diagram of different blueberry varieties

    表  1   不同品种蓝莓形态指标比较

    Table  1   Comparison of morphological indexes of different varieties of blueberries

    品种单果质量(g)横径(mm)纵径(mm)果形指数果实形状
    莱格西2.01±0.03a13.44±0.77b10.25±0.59b0.76±0.04c扁圆形
    绿宝石1.86±0.09b13.00±0.86c9.86±0.75b0.76±0.05c扁圆形
    密斯梯1.35±0.06c11.21±0.77d8.13±0.60d0.72±0.03d扁圆形
    蓝雨0.85±0.02e9.69±0.52e8.90±0.44c0.92±0.04a椭圆形
    布里吉塔2.06±0.07a14.39±0.50a11.32±0.90a0.79±0.05c扁圆形
    园蓝1.09±0.08d10.85±0.51d8.99±0.63c0.83±0.05b圆形
    注:同列不同字母表示差异显著(P<0.05),表2~表5同。
    下载: 导出CSV

    表  2   不同品种蓝莓色度比较

    Table  2   Comparison of chroma of different blueberries

    品种L*a*b*
    莱格西41.74±4.6a1.18±0.11bc-6.09±1.42b
    绿宝石42.86±3.46a1.49±0.51ab-6.10±1.17b
    密斯梯37.07±4.03b1.74±0.10a-5.99±0.95b
    蓝雨28.75±2.52c1.02±0.25c-4.84±0.75a
    布里吉塔25.30±1.98d1.43±0.95ab-4.63±1.36a
    园蓝35.13±2.79b0.65±0.06d-5.19±0.84ab
    下载: 导出CSV

    表  4   不同品种蓝莓的水分含量、TSS、还原糖、可溶性糖、总酸及糖酸比

    Table  4   Water content, TSS, reducing sugar, soluble sugar, total acid and sugar-acid ratio of different blueberries

    品种水分含量(%)TSS(%)还原糖(%)可溶性糖(%)总酸(%)糖酸比
    莱格西85.96±0.16bc10.7±0.12d9.66±1.49ab10.63±0.77b1.02±0.1b10.48±1.38bc
    绿宝石88.11±0.83a11.3±0.23c7.81±0.45cd11.04±1.26ab1.14±0.01a9.66±2.87c
    密斯梯86.53±0.51b11.9±0.12b9.99±0.07a11.70±1.84ab1.00±0.10b11.74±1.89b
    蓝雨85.28±0.15c10.5±0.12e7.37±0.32d8.93±0.07c1.18±0.03a7.59±0.18d
    布里吉塔87.72±0.35a11.1±0.12c8.38±0.87bcd10.51±0.40bc1.07±0.01ab9.86±0.41bc
    园蓝80.83±0.53d14.9±0.12a8.99±0.42abc12.61±0.48a0.45±0.02c28.23±0.46a
    下载: 导出CSV

    表  3   不同品种蓝莓质构比较

    Table  3   Texture comparison of different blueberry varieties

    品种硬度(g)弹性(mm)胶粘性(N)咀嚼性(mJ)
    莱格西715.31±90.61b0.69±0.03a280.33±41.8a183.67±26.75a
    绿宝石741.75±120.21b0.63±0.07b246.59±36.6b162.55±24.19b
    密斯梯439.41±80.36d0.69±0.03a170.33±15.88d114.44±12.06c
    蓝雨805.67±68.21a0.65±0.02ab296.48±24.82a193.86±19.31a
    布里吉塔408.37±58.17d0.66±0.06ab151.96±20.40d102.58±13.49c
    园蓝510.65±55.66c0.69±0.09a225.74±20.53c155.33±19.93b
    下载: 导出CSV

    表  5   不同品种蓝莓花青素、总酚、黄酮含量比较

    Table  5   Comparison of anthocyanins, total phenols and flavonoids contents in different blueberries

    品种花青素(mg/g)总酚(mg/g)黄酮(mg/g)
    莱格西0.84±0.23c2.35±0.2c0.68±0.06b
    绿宝石0.97±0.18c2.54±0.25c0.58±0.02b
    密斯梯1.05±0.17c3.07±0.13b0.65±0.01b
    蓝雨1.36±0.11b3.01±0.07b0.33±0.03d
    布里吉塔0.92±0.14c1.64±0.04d0.45±0.04c
    园蓝2.09±0.10a5.78±0.39a0.99±0.13a
    下载: 导出CSV

    表  6   不同品种蓝莓游离氨基酸组成

    Table  6   Free amino acid composition of different blueberries

    游离氨基酸
    (mg/100 g)
    莱格西绿宝石密斯梯蓝雨布里吉塔园蓝
    天冬氨酸Asp8.128±0.78b8.319±0.63b10.989±0.71a8.767±1.34ab7.240±0.84b9.128±0.77ab
    苏氨酸Thr1.690±0.62b1.971±0.78ab3.103±0.70a2.108±0.71ab1.84±0.57b2.688±0.65a
    丝氨酸Ser5.019±0.72a6.735±1.40a5.882±1.41a6.025±1.40a6.1±1.48a5.382±0.64a
    天冬酰胺Asn1.752±0.70c4.115±0.74b29.074±1.40a4.843±1.39b4.135±1.40b4.681±2.14b
    谷氨酸Glu10.556±0.70c16.072±1.41b28.187±0.71a14.889±2.11b14.339±1.42b30.914±1.35a
    甘氨酸Gly0.443±0.44b0.353±0.21c0.576±0.35a
    丙氨酸Ala12.722±1.41a12.560±2.11a11.155±0.08a11.797±1.41a12.566±1.34a7.364±0.91b
    缬氨酸Val3.710±1.41b3.071±0.64c4.058±1.49a2.825±0.85d
    异亮氨酸Ile0.730±0.27a0.717±0.33a
    亮氨酸Leu0.772±0.28a0.760±0.26a
    γ-氨基丁酸GABA27.944±0.28a28.626±2.12a17.028±2.11d24.561±1.38ab19.897±1.56cd22.158±0.77bc
    组氨酸His1.761±0.82b2.675±2.12ab5.030±1.43a1.549±0.76b0.929±0.05b0.639±0.50b
    赖氨酸Lys36.874±1.41b38.052±1.41a42.145±2.05a30.590±1.40c19.015±0.64d5.970±1.45e
    精氨酸Arg60.693±0.79c212.721±3.54a51.791±2.13d24.516±1.34e89.471±1.28b
    脯氨酸Pro73.090±1.41b66.349±1.42c66.575±1.43c83.612±1.40a45.865±2.22d
    苯丙氨酸Phe0.737±0.27
    胱氨酸(Cys)23.810±1.40a1.995±0.84c3.450±0.73b
    酪氨酸Tyr7.876±1.41
    必需氨基酸占比(%)17.800±0.21b35.650±0.40a11.320±0.33d14.360±0.30c11.900±0.20d4.260±0.03e
    游离氨基酸总量245.880±4.13b122.930±3.21f435.720±4.33a227.660±5.16d199.010±2.18e237.640±3.25c
    注:同行不同小写字母表示差异显著(P<0.05),表7同。
    下载: 导出CSV

    表  7   不同品种蓝莓挥发性风味物质组成(ng/g)

    Table  7   Composition of volatile flavor compounds in different varieties of blueberries (ng/g)

    类别CAS号化合物品种
    布里
    吉塔
    莱格西蓝雨绿宝石密斯梯园蓝
    醇类470-82-6桉叶油醇593.93±9.59
    78-70-6芳樟醇1123.29±10.13c124.92±4.10f629.74±7.81e1454.75±12.10b949.27±9.12d6621.32±15.21a
    98-55-5alpha-松油醇31.58±1.02d278.88±3.34b201.95±1.23c581.89±4.66a
    7341-17-52-乙基己基硫醇159.65±1.02b203.29±3.41a52.56±0.46d61.77±0.82c
    97-99-4四氢糠醇34.27±0.11
    18409-18-2反式-2-癸烯醇52.04±0.13
    536-59-4紫苏醇26.17±0.09
    7786-44-92,6-壬二烯醇666.71±10.21b1329.42±13.20a
    5921-73-32-壬炔-1-醇1257.75±16.21
    34995-77-2三甲基-5-乙烯基四氢化-2-呋喃甲醇210.22±2.46
    74962-98-4反-2-十三烯醇32.22±0.21
    112-53-8十二醇22.85±0.34
    醛类100-52-7苯甲醛179.07±1.23b63.25±0.43c172.37±1.66b280.74±3.25a
    2548-87-0反-2-辛烯醛52.37±0.45
    124-19-6壬醛216.30±2.71a205.13±1.96b206.29±3.77b
    557-48-2反,顺-2,6-壬二烯醛21.73±0.70
    18829-56-6反式-2-壬烯醛56.55±0.45c116.21±1.21a73.58±0.48b
    112-31-2癸醛90.85±1.02f150.79±2.70d119.19±0.98e223.01±4.21b169.68±2.33c269.42±3.22a
    60784-31-8反-2-壬烯醛44.79±0.22c94.67±1.09a78.86±0.37b42.15±0.34c
    124-13-0正辛醛101.10±0.77
    112-54-9十二醛44.64±0.34
    141-27-5顺-3,7-二甲基-2,6-辛二烯醛49.70±0.36b80.60±0.93a
    萜烯类123-35-3月桂烯476.75±10.30d521.18±9.89b493.36±12.30c570.75±10.99a
    13877-91-3罗勒烯94.17±0.46e264.15±6.44c214.21±4.81d277.84±3.77b279.19±2.35b558.63±6.70a
    4316-65-83,5,5-三甲基-1-己烯58.74±0.53
    7785-26-4左旋-alpha-蒎烯218.58±2.21a168.00±1.70b
    586-62-9萜品油烯61.54±0.22b61.26±0.19b55.37±0.09c61.63±0.16b144.04±1.33a
    5392-40-5柠檬醛147.88±1.33
    87-44-51-石竹烯382.21±3.66a39.75±1.22c50.23±0.44b
    6753-98-6α-石竹烯78.78±0.78
    3338-55-4反-3,7-二甲基-1,3,6-十八烷三烯47.88±1.02
    1139-30-6氧化石竹烯27.75±0.50a13.41±0.06b
    5989-54-8(-)-柠檬烯48.98±0.80b1005.06±17.99a
    11028-42-5雪松烯77.59±1.55b160.00±2.52a
    489-39-4(+)-香橙烯26.74±0.39b92.31±0.81a25.34±0.08b
    5989-27-5右旋萜二烯874.75±10.02
    138-86-3双戊烯413.40±5.11
    29548-02-5黏蒿三烯197.79±1.44
    1195-92-2(R)-氧化柠檬烯58.25±0.62
    6931-54-0beta-环氧蒎烷41.04±0.09
    77-53-2柏木脑33.44±0.07
    2437-56-11-十三烯9.16±0.41
    酯类13361-34-7氰乙酸异辛酯29.56±0.11b42.56±0.41a
    103-11-7丙烯酸2-乙基己酯59.52±0.72e195.99±1.01c258.17±3.07b208.77±2.27c122.60±1.44d288.69±2.93a
    6846-50-02,2,4-三甲基-1,3-戊二醇二异丁酸酯61.89±0.60f123.61±1.02d262.23±2.33a179.46±0.88c205.99±1.63b109.15±1.00e
    84-69-5邻苯二甲酸二异丁酯67.91±0.17a11.73±0.03b
    23853-33-0硫代苯甲酸S-羟甲酯25.91±0.28b57.79±0.51a
    25415-84-32-乙基己酸乙酯85.05±0.72a64.54±0.51c71.76±0.40b28.34±0.05e56.80±0.34d
    141-12-8乙酸橙花酯21.13±0.02
    2566-89-4花生四烯酸甲酯46.21±0.15a24.76±0.04b30.73±0.21c
    1731-88-0十三碳酸脂肪酸43.34±0.11a42.26±0.17a34.10±0.12b
    111-82-0月桂酸甲酯90.66±1.03b43.71±0.18c113.50±0.77a
    110-42-9癸酸甲酯33.28±0.24b16.57±0.04c83.95±0.60a
    110-38-3癸酸乙酯76.25±0.35
    115-99-13,7-二甲基-1,6-辛二烯-3-醇甲酸酯57.20±0.15
    酮类112-12-9甲基壬基甲酮127.08±1.22d387.23±2.88b231.92±1.55c238.54±3.03c475.42±3.50a47.26±0.22e
    3796-70-1香叶基丙酮160.82±1.33b56.58±0.51d77.71±0.40c175.14±0.91a51.88±0.49e
    821-55-62-壬酮235.09±2.11a80.24±0.71c133.05±0.34b53.72±0.23d
    464-48-2左旋樟脑123.43±2.03
    2345-27-92-十四酮90.45±0.30
    6485-40-1左旋香芹酮145.00±1.39
    其他95-47-6邻二甲苯198.51±1.08
    629-20-9环辛四烯710.75±9.22c647.15±4.70d860.01±10.33b332.56±4.12e325.96±2.09f1024.07±15.23a
    629-50-5正十三烷17.92±0.03e182.47±0.40b61.31±0.12c40.51±0.06d215.79±1.13a
    544-76-3十六烷18.72±0.03d19.97±0.01d47.85±0.20b26.34±0.33c116.96±0.73a
    112-40-3十二烷18.39±0.02f81.07±0.53c113.16±0.77b33.26±0.11e43.38±0.21d164.23±1.00a
    1120-21-4十一烷39.11±0.06b34.58±0.03b74.61±0.12a37.30±0.01b
    119-36-8水杨酸甲酯142.91±1.02c160.12±1.34b287.87±2.11a
    15869-93-93,5-二甲基辛烷44.19±0.40
    124-18-5癸烷71.63±0.40
    5333-84-63-甲基四氢苯酐416.90±6.11a365.93±4.03b
    1138-52-93,5-二叔丁基苯酚28.89±0.02
    629-59-4十四烷40.07±0.05
    总量4466.41±16.42e1618.06±12.02f4942.14±18.29d7052.68±23.01b5800.32±20.44c14604.72±17.96a
    下载: 导出CSV

    表  8   主成分特征值及累计贡献率

    Table  8   Principal component characteristic values and cumulative contribution rate

    主成分特征值贡献率(%)累计贡献率(%)
    18.83444.16944.169
    25.60628.02972.198
    33.40517.02589.223
    下载: 导出CSV

    表  9   主成分载荷表

    Table  9   Principal component load table

    指标成分
    123
    单果重(X1−0.443−0.6530.122
    果形指数(X20.0590.939−0.292
    L*(X30.119−0.2770.910
    a*(X4−0.634−0.633−0.019
    b*(X5−0.209−0.051−0.887
    硬度(X6−0.3480.6920.620
    弹性(X70.583−0.4050.119
    胶粘性(X8−0.090.7340.65
    咀嚼性(X9−0.0250.7430.644
    水分含量(X10−0.907−0.35−0.005
    TSS(X110.972−0.072−0.125
    还原糖(X120.361−0.7410.279
    可溶性糖(X130.781−0.5620.083
    总酸(X14−0.9830.0690.059
    糖酸比(X150.986−0.012−0.064
    花青素(X160.8730.445−0.181
    总酚(X170.9480.2710.055
    黄酮(X180.897−0.3050.287
    游离氨基酸总量(X19−0.1670.833−0.438
    风味化合物总量(X200.8740.166−0.190
    下载: 导出CSV

    表  10   不同品种蓝莓主成分评分和综合评分

    Table  10   Principal component score and comprehensive score of different blueberries

    品种F1F2F3F排名
    园蓝5.8460.755−0.3713.0601
    蓝雨−1.7524.215−0.4830.3652
    莱格西−0.863−0.8722.499−0.2243
    绿宝石−1.585−0.0091.325−0.5354
    密斯梯0.252−2.580−0.029−0.6925
    布里吉塔−1.898−1.508−2.941−1.9746
    下载: 导出CSV
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    KALT W, CASSIDY A, HOWARD L R, et al. Recent research on the health benefits of blueberries and their anthocyanins[J]. Advances in Nutrition,2020,11(2):224−236.

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  • 收稿日期:  2021-11-16
  • 网络出版日期:  2022-05-01
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