Abstract: The intestinal tract is not only the main part of nutrient digestion and absorption, but also an important immune and endocrine organ. The digestion and absorption of nutrients in the intestine is a crucial factor to achieve their efficient utilization. Constructing an appropriate in vitro intestinal model is of great significance to clarify the effective absorption site, absorption efficiency, and mechanism of nutrients. Organoids are widely used in various fields because they can highly simulate the genetic and apparent characteristics of target tissues or organs. This paper reviews the recent application research progress of intestinal organoids in nutrient digestion and absorption, with a view to providing a reference for the wide application of intestinal organoids in nutrient digestion and absorption.