Abstract: Objective: To study the anti-alcoholic and hepatoprotective effect of Shanxi Maojian tea aqueous extract, in order to provide the evidence for its further development and utilization. Methods: Kunming mice were randomly divided into 5 groups: Drunken model group, naloxone hydrochloride group (0.002 g/kg), high-dose Maojian tea group (3 g/kg), medium-dose Maojian tea group (1.5 g/kg), low-dose Maojian tea group (0.75 g/kg). Two hours after single gavage of Maojian tea by dosage of administration, each group was given the corresponding 50% alcohol (20 mL/kg), and 30 minutes after gavage with 50% alcohol, naloxone hydrochloride group was intraperitoneally injected with naloxone hydrochloride injection. The ebriety time and the sober time of drunken mice in each group were recorded. Sixty SD rats were randomly divided into 6 groups: Control group, chronic alcoholic liver injury model group, Dongbaogantai group (0.36 g/kg), Maojian tea high-dose, medium-dose and low-dose groups. Except for control group, the other groups gavage with 50% alcohol in the morning. In the afternoon, Dongbaogantai group and Maojian tea groups were given the corresponding drugs by continuous administration for 6 weeks. After the experiment, the contents of glutamate aminotransferase (ALT), aspartate aminotransferase (AST), cholesterol (TC), triglyceride (TG) in serum were detected, and the levels of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione (GSH) in liver were detected and pathological observation was taken after H&E staining with liver of rats. Results: Compared with the drunken model group, the sober time of Maojian tea groups were significantly shortened (P<0.01). Compared with control group, the levels of ALT, AST, TC, TG in chronic alcoholic liver injury model group were significantly increased (P<0.05), and MDA significantly increased (P<0.01), while the levels of SOD and GSH were significantly decreased (P<0.01). Compared with alcoholic liver injury group, the levels of AST, TC and MDA in Maojian tea high-dose group were significantly decreased (P<0.01), the levels of SOD and GSH were significantly increased (P<0.01), and the levels of ALT in Maojian tea medium-dose group were significantly decreased (P<0.05), AST content significantly decreased (P<0.01), SOD and GSH significantly increased (P<0.01), the levels of AST and TG in Maojian tea low-dose group were significantly decreased (P<0.01), and the levels of SOD and GSH were significantly increased (P<0.01). H&E staining results showed that the pathological changes of liver tissues in different doses of Maojian tea groups could be improved to different degrees. Conclusion: Maojian tea had anti-alcoholic and hepatoprotective effect.