Abstract: C₉~C₃₃ n-alkane standard solution and Smart Database Pesticides database were used to establish a rapid analytical method for the determination of 32 fungicides in strawberry based on retention index. Thirty-two fungicides were selected as the target, the samples were extracted and purified by QuEChERS method. Gas chromatography tandem mass spectrometry was used for qualitative and quantitative analysis. Results showed that, the 32 fungicides showed good linear relationship in the range of 5~200 μg/L, and the correlation coefficients were greater than 0.9982. The recoveries of 32 fungicides spiked at 10, 50, 100 μg/kg were 70.3%~117.8% and therelative standard deviations were 0.01%~8.50%. The limits of detection were 0.01~2.91 μg/kg, and the limits of quantitative were 0.04~9.71 μg/kg. This method was rapid, simple, accurate, steady, and achieved good performance in strawberry sample analyses, and was suitable for monitoring fungicides using in strawberry, and provided reliable date and technical support for determination of multi-component pesticide residues studies．