Abstract: The antioxidant, antibacterial and antiproliferative activities of different solvents (chloroform, ethyl acetate, n-butanol, ethanol) extracts from Ophiolossum vulgatum L. were compared. The antioxidant activity of four extracts was evaluated by determining the removal effect of three free radicals, with ascorbic acid used as control. The antimicrobial activity of the four extracts against several common pathogenic bacteria was determined by microdilution assay. The antiproliferative activities on SKOV3 cells were detected by MTT assay. The results showed that all the extracts had certain antioxidant, antibacterial and antiproliferative activities, especially n-butanol extract exhibited the strongest activity among them. The scavenging effect of n-butanol extract was the best for DPPH free radicals, with IC₅₀ value of 0.114 mg/mL, and it had obvious inhibitory effect on beta hemolytic Streptococcus, Pseudomonas aeruginosa and Monilia albican, with MIC value of 0.313 mg/mL, and had the strongest inhibitory effect on SKOV3 ovarian cancer cells, with IC₅₀ value of 114.5 μg/mL. The n-butanol extract from Ophiolossum vulgatum L. showed the potential to be further studied and used in the fields of drugs and functional foods.