沙棘粕槲皮素体外抗氧化及对衰老模型小鼠保护作用的研究

沈晓溪 张一鸣 赵梓伊 石晨晖 尚宏丽

沈晓溪,张一鸣,赵梓伊,等. 沙棘粕槲皮素体外抗氧化及对衰老模型小鼠保护作用的研究[J]. 食品工业科技,2021,42(20):350−356. doi:  10.13386/j.issn1002-0306.2021020011
引用本文: 沈晓溪,张一鸣,赵梓伊,等. 沙棘粕槲皮素体外抗氧化及对衰老模型小鼠保护作用的研究[J]. 食品工业科技,2021,42(20):350−356. doi:  10.13386/j.issn1002-0306.2021020011
SHEN Xiaoxi, ZHANG Yiming, ZHAO Ziyi, et al. Study on Antioxidant Activity of Quercetin from Seabuckthorn Meal in Vitro and Its Protective Effect on Aging Mice[J]. Science and Technology of Food Industry, 2021, 42(20): 350−356. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2021020011
Citation: SHEN Xiaoxi, ZHANG Yiming, ZHAO Ziyi, et al. Study on Antioxidant Activity of Quercetin from Seabuckthorn Meal in Vitro and Its Protective Effect on Aging Mice[J]. Science and Technology of Food Industry, 2021, 42(20): 350−356. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2021020011

沙棘粕槲皮素体外抗氧化及对衰老模型小鼠保护作用的研究

doi: 10.13386/j.issn1002-0306.2021020011
基金项目: 辽宁省自然科学基金项目(20180550549)
详细信息
    作者简介:

    沈晓溪(1998−),女,硕士研究生,研究方向:食品加工与安全,E-mail:2300929979@qq.com

    通讯作者:

    尚宏丽(1977−),女,博士,副教授,研究方向:食品生物技术,E-mail:shanghongli007@126.com

  • 中图分类号: TS255

Study on Antioxidant Activity of Quercetin from Seabuckthorn Meal in Vitro and Its Protective Effect on Aging Mice

  • 摘要: 以沙棘粕槲皮素为研究对象,探索其体外抗氧化活性和对衰老模型小鼠的保护作用。采用1, 1-二苯基-2-三硝基苯肼(DPPH·)法、超氧阴离子(${\rm{O}}_2^- \cdot$)法、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS+·)法、高铁离子还原(FRAP)法,四种方法对沙棘果槲皮素体外抗氧化活性进行研究。通过颈部皮下注射D-半乳糖建立小鼠衰老模型,用不同剂量的(100、250、500 mg/kg)沙棘粕槲皮素灌胃处理小鼠,眼球取血测血清中总抗氧化能力(T-AOC)、谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量;取心脏、肾脏和肝脏做苏木精-伊红染色(HE)病理学切片,观察其组织细胞病理状态。结果表明:沙棘粕槲皮素对DPPH·、${\rm{O}}_2^- \cdot $、ABTS+·具有良好的清除能力。与模型组比较,沙棘粕槲皮素低剂量组的MDA含量显著降低(P<0.05),中、高剂量组小鼠血清中T-AOC、GSH-PX、SOD活力显著升高(P<0.05),MDA含量显著降低(P<0.05)。从病理组织切片看,随着沙棘粕槲皮素剂量的增加,心肌纤维排列有序,横纹清晰;肾小囊和肾小球形态逐渐正常,肾小管数量增多且排列紧密;肝细胞数量增多,细胞形态完整,窦间隙逐渐显著。由此可知,沙棘粕槲皮素能提高衰老模型小鼠抗氧化酶活性和还原性物质含量,降低脂质过氧化物含量,从而提高衰老模型小鼠的抗氧化能力,对衰老模型小鼠有较好的保护作用。
  • 图  1  沙棘粕槲皮素、VC和芦丁对DPPH·的清除能力

    Figure  1.  Scavenging ability of quercetin, VC and rutin from seabuckthorn meal on DPPH·

    图  2  沙棘粕槲皮素、VC和芦丁对${\rm{O}}_2^- \cdot $的清除能力

    Figure  2.  Scavenging ability of quercetin, VC and rutin from seabuckthorn meal on ${\rm{O}}_2^- \cdot $

    图  3  沙棘粕槲皮素、VC和芦丁对ABTS+·的清除能力

    Figure  3.  Scavenging ability of quercetin, VC and rutin from seabuckthorn meal on ABTS+·

    图  4  沙棘粕槲皮素、VC和芦丁的还原力

    Figure  4.  Reducing power of quercetin, VC and rutin in seabuckthorn meal

    图  5  各组小鼠心脏组织切片HE染色结果(400×)

    Figure  5.  Results of tissue slicing of heart sections by HE staining in each group(400×)

    注:A~F分别表示正常组,模型组,阳性对照组,槲皮素低、中、高剂量组。图中箭头表示心肌细胞。

    图  6  各组小鼠肾脏组织切片HE染色结果(400×)

    Figure  6.  Results of tissue slicing of kidney sections by HE staining in each group(400×)

    注:A~F分别表示正常组,模型组,阳性对照组,槲皮素低、中、高剂量组。1:肾小球;2:肾小囊。

    图  7  各组小鼠肝脏组织切片HE染色结果(400×)

    Figure  7.  Results of tissue slicing of liver sections by HE staining in each group(400×)

    注:A~F分别表示正常组,模型组,阳性对照组,槲皮素低、中、高剂量组。1:细胞核;2:包浆颗粒物;3:窦间隙。

    表  1  沙棘粕槲皮素对衰老小鼠血清中T-AOC、GSH-PX、SOD活力及MDA含量的影响

    Table  1.   Effect of seabuckthorn fruit quercetin on the activities of T-AOC、GSH-PX、SOD and the content of MDA in serum of mice

    组别T-AOC(U/mL)GSH-PX(U/mL)SOD(U/mL)MDA(U/mL)
    正常组18.359±2.47160.791±5.647134.758±8.8916.257±1.394
    模型组7.145±1.492**32.835±3.497**97.869±6.374**15.839±1.975**
    阳性对照组15.794±3.056##58.347±4.739##126.218±8.537##7.024±1.206##
    低剂量组9.478±1.05341.153±6.374100.339±4.79612.174±0.958#
    中剂量组11.579±0.974#52.818±5.079##109.758±5.263#9.943±1.472##
    高剂量组14.078±2.058##58.493±4.925##128.357±8.279##7.938±1.253##
    注:与正常组相比,*P<0.05,**P<0.01;与模型组相比,#P<0.05,##P<0.01。
    下载: 导出CSV
  • [1] 焦岩, 常影, 余世锋, 等. 大果沙棘总黄酮体外抗氧化和抑菌作用研究[J]. 食品研究与开发,2015,36(19):12−15. [Jiao Yan, Chang Ying, Yu Shifeng, et al. In vitro antioxidant and antibacterial activities of total flavonoids from Hippophae rhamnoides[J]. Food Research and Development,2015,36(19):12−15. doi:  10.3969/j.issn.1005-6521.2015.19.004
    [2] 张佳婵, 王昌涛, 赵丹, 等. 沙棘粕醇提物的定性定量分析及其对衰老小鼠肝脏抗氧化指标的影响[J]. 食品科学,2019,40(2):229−238. [Zhang Jiachan, Wang Changtao, Zhao Dan, et al. Qualitative and quantitative analysis of ethanol extract from seabuckthorn meal and its effect on liver antioxidant indexes of aging mice[J]. Food Science,2019,40(2):229−238. doi:  10.7506/spkx1002-6630-20180614-285
    [3] 张佳婵, 王昌涛, 易思雨, 等. 沙棘粕提取物对H2O2诱导的B16F10细胞氧化损伤的保护及修复[J]. 中国食品学报,2019,19(8):13−21. [Zhang Jiachan, Wang Changtao, Yi Siyu, et al. Protection and repair of seabuckthorn meal extract on H2O2 induced oxidative damage in B16F10 cells[J]. Chinese Journal of Food,2019,19(8):13−21.
    [4] Rivera L, R Morón, M Sánchez, et al. Quercetin ameliorates metabolic syndrome and improves the inflammatory status in obese Zucker rats[J]. Obesity,2012,16(9):2081−2087.
    [5] 游庭活, 温露, 刘凡. 衰老机制及延缓衰老活性物质研究进展[J]. 天然产物研究与开发,2015,27(11):1985−1990. [You Tinghuo, Wen LU, Liu Fan. Progress in the study of aging mechanism and anti-aging active substances[J]. Research and Development of Natural Products,2015,27(11):1985−1990.
    [6] Vetter J, P Novák, Wagner M R, et al. Ageing mechanisms in lithium-ion batteries[J]. Journal of Power Sources,2005,147(1-2):269−281. doi:  10.1016/j.jpowsour.2005.01.006
    [7] Jordi M, Czrina C, Gan W B, et al. Abnormal mitochondrial transport and morphology are common pathological denominators in SOD1 and TDP43 ALS mouse models[J]. Human Molecular Genetics,2014(6):1413−1424.
    [8] 段亚云, 李建颖, 程瑶, 等. 黑果枸杞叶槲皮素的制备及其体外抗氧化活性研究[J]. 食品研究与开发,2019,40(5):59−64. [Duan Yayun, Li Jianying, Cheng Yao, et al. Preparation of quercetin from Lycium ruthenicum leaves and its antioxidant activity in vitro[J]. Food Research and Development,2019,40(5):59−64. doi:  10.3969/j.issn.1005-6521.2019.05.011
    [9] 黄思莹, 黄春萍, 刘旭, 等. 桑叶槲皮素对D-半乳糖致衰老小鼠抗氧化能力及肠道菌群的影响[J]. 四川师范大学学报(自然科学版),2020,43(3):373−377. [Huang Siying, Huang Chunping, Liu Xu, et al. Effects of quercetin from mulberry leaves on antioxidant capacity and intestinal microflora in aging mice induced by D-galactose[J]. Journal of Sichuan Normal University (Natural Science Edition),2020,43(3):373−377.
    [10] Wang Y, Yu H, Wang S, et al. Targeted delivery of quercetin by nanoparticles based on chitosan sensitizing paclitaxel-resistant lung cancer cells to paclitaxel[J]. Materials Science and Engineering C,2020,119:111442.
    [11] 吴春, 徐丽萍, 聂芊. 槲皮素-β-环糊精包合物作为亚硝化反应的抑制剂研究[J]. 食品与发酵工业,2007(7):78−80. [Wu Chun, Xu Liping, Nie Qian. Study on quercetin-cyclodextrin inclusion complex as an inhibitor of nitrosation[J]. Food and Fermentation Industry,2007(7):78−80.
    [12] Lu J, Papp L V, Fang J, et al. Inhibition of mammalian thioredoxin reductase by some flavonoids: Implications for myricetin and quercetin anticancer activity[J]. Cancer Research,2006,66(8):4410−4418. doi:  10.1158/0008-5472.CAN-05-3310
    [13] Zheng Yanyan, He Ruonan, Chen Zhongwei, et al. Optimization of extraction process of quercetin from flos sophorae immaturus using orthogonal design[J]. Medicinal Plant,2020,11(6):67−71.
    [14] 童钰琴, 李姝, 牛曼思, 等. 苦荞麸皮总黄酮体外抗氧化活性及体内解酒护肝作用[J]. 食品工业科技,2020,41(17):314−319, 326. [Tong Yuqin, Li Shu, Niu Mansi, et al. Antioxidant activity of total flavonoids from buckwheat bran and its in vivo action of relieving wine and protecting liver[J]. Food Industry Technology,2020,41(17):314−319, 326.
    [15] 孙丽萍, 王大仟, 张智武. 11种天然植物提取物对DPPH自由基的清除作用[J]. 食品科学,2009,30(1):45−47. [Sun Liping, Wang Daqian, Zhang Zhiwu. Scavenging effect of 11 natural plant extracts on DPPH free radical[J]. Food Science,2009,30(1):45−47. doi:  10.3321/j.issn:1002-6630.2009.01.010
    [16] Zhou G, Lv G. Comparative studies on scavenging DPPH free radicals activity of flavone C-glycosides from different parts of Dendrobium officinale[J]. China Journal of Chinese Materia Medica,2012,37(11):1536−1540.
    [17] 胡月芳. 淮山多糖超声辅助提取及清除超氧阴离子自由基的作用[J]. 江苏农业科学,2017,45(4):144−146. [Hu Yuefang. Ultrasonic assisted extraction of polysaccharides from Huaishan mountain and its scavenging effect on superoxide anion radical[J]. Jiangsu Agricultural Sciences,2017,45(4):144−146.
    [18] Wu C Q, Chen G H. Detection of synergistic effect of superoxide dismutase and jujubosides on scavenging superoxide anion radical by capillary electrophoresis[J]. Biomedical Chromatography,2019,33(10):e4630.
    [19] 孟庆焕, 王化, 王洪政, 等. 牡丹种皮黄酮提取及对ABTS自由基清除作用[J]. 植物研究,2013,33(4):504−507. [Meng Q H, Wang H, Wang H Z, et al. Extraction of flavonoids from peony seed coat and its scavenging effect on ABTS free radical[J]. Plant Research,2013,33(4):504−507. doi:  10.7525/j.issn.1673-5102.2013.04.020
    [20] Liu Z, Shi Y, Liu H, et al. Purification and identification of pine nut (Pinus yunnanensis Franch.) protein hydrolysate and its antioxidant activity in vitro and in vivo[J]. Chemistry & Biodiversity,2021,18:e2000710.
    [21] 王蔚新, 张俊, 占剑峰, 等. 福白菊胎菊与朵菊粗多糖体外抗氧化活性分析[J]. 黄冈师范学院学报,2020,40(6):9−13. [Wang Weixin, Zhang Jun, Zhan Jianfeng, et al. In vitro antioxidant activity of crude polysaccharides from Chrysanthemum morifolium and Chrysanthemum morifolium[J]. Journal of Huanggang Normal University,2020,40(6):9−13. doi:  10.3969/j.issn.2096-7020.2020.06.02
    [22] 毕凯媛, 李娜, 崔珊珊, 等. 红树莓花色苷对D-半乳糖衰老模型小鼠的保护作用[J]. 食品工业科技,2019,40(6):279−284. [Bi Kaiyuan, Li Na, Cui Shanshan, et al. The protective effect of anthocyanin of rubbingus on D-galactose aging model mice[J]. Food Industry Technology,2019,40(6):279−284.
    [23] 王兵亚, 连秀仪, 杨琛擘, 等. 夏黑葡萄花青素抗氧化活性及对D-半乳糖衰老模型小鼠的保护作用[J]. 食品工业科技,2020,41(3):22−26, 32. [Wang Bingya, Lian Xiuyi, Yang Chenbo, et al. Antioxidant activity of anthocyanins from Xiahei grape and its protective effect on D-galactose aging model mice[J]. Food Industry Science and Technology,2020,41(3):22−26, 32.
    [24] 孙玉军, 江昌俊, 任四海. 秀珍菇多糖对D-半乳糖致衰老小鼠的保护作用[J]. 食品科学,2017,38(5):251−256. [Sun Yujun, Jiang Changjun, Ren Sihai. Protective effect of Pleurotus ostreatus polysaccharide on aging mice induced by D-galactose[J]. Food Science,2017,38(5):251−256. doi:  10.7506/spkx1002-6630-201705041
    [25] 石艳宾, 鲍佳彤, 王亦萱, 等. 金银花绿原酸与芦丁、槲皮素协同抗氧化作用[J]. 食品工业,2020,41(5):199−202. [Shi Yanbin, Bao Jiatong, Wang Yixuan, et al. Synergistic antioxidant effect of chlorogenic acid of honeysuckle with rutin and quercetin[J]. Food Industry,2020,41(5):199−202.
    [26] 田建华, 张春媛, 魏璐. 沙棘果渣总黄酮提取工艺优化及抗氧化活性研究[J]. 天然产物研究与开发,2021,33(1):65−72. [Tian Jianhua, Zhang Chunyuan, Wei Lu. Optimization of extraction technology and antioxidant activity of total flavonoids from seabuckthorn fruit residue[J]. Research and Development of Natural Products,2021,33(1):65−72.
    [27] 肖颖, 余英才, 余雄英, 等. 异鼠李素和槲皮素抗氧化活性研究[J]. 时珍国医国药,2012,23(5):1118−1120. [Xiao Ying, Yu Yingcai, Yu Xiongying, et al. Study on the antioxidant activities of isorhamnetin and quercetin[J]. Shizhen National Medicine,2012,23(5):1118−1120. doi:  10.3969/j.issn.1008-0805.2012.05.031
    [28] He M, Zhao L, Wei M J, et al. Neuroprotective effects of (-)-epigallocatechin-3-gallate on aging mice induced by D-galactose[J]. Biological & Pharmaceutical Bulletin,2009,32(1):55−60.
    [29] Pei L P, Dong F H. Effect of flavone of Pueraria lobata Ohwi on osseous changes in the aged rats induced by D-galactose[J]. Chinese Journal of Gerontology,2007:1750−1753.
    [30] 张勍, 汪晟坤, 连秀仪, 等. 夏黑葡萄花青素在D-半乳糖致衰老小鼠心肌细胞抗氧化和清除自由基中的作用[J]. 河南医学研究,2019,28(20):3649−3652. [Zhang Qiong, Wang Shengkun, Lian Xiuyi, et al. Effect of anthocyanins from Xiahei grape on antioxidation and free radical scavenging of cardiomyocytes in aging mice induced by D-galactose[J]. Henan Medical Research,2019,28(20):3649−3652. doi:  10.3969/j.issn.1004-437X.2019.20.001
    [31] 包晓玮, 李建瑛, 任薇, 等. 沙棘多糖对D半乳糖致衰老小鼠的抗氧化作用[J]. 食品工业科技,2020,41(4):293−297, 306. [Bao Xiaowei, Li Jianying, Ren Wei, et al. Antioxidant effect of seabuckthorn polysaccharide on aging mice induced by D-galactose[J]. Food Industry Science and Technology,2020,41(4):293−297, 306.
  • 加载中
图(7) / 表(1)
计量
  • 文章访问数:  36
  • HTML全文浏览量:  10
  • PDF下载量:  1
  • 被引次数: 0
出版历程
  • 收稿日期:  2021-02-02
  • 网络出版日期:  2021-09-13

目录

    /

    返回文章
    返回

    重要通知

    《食品工业科技》编辑部携手万方数据开通学术不端专属检测通道,具体信息参见本刊动态。