簕菜提取物对酒精性肝损伤大鼠的保护作用

李冬梅 李志阳 杨君 郝志明 杨洁 林春华

李冬梅,李志阳,杨君,等. 簕菜提取物对酒精性肝损伤大鼠的保护作用[J]. 食品工业科技,2021,42(18):380−386. doi:  10.13386/j.issn1002-0306.2021010238
引用本文: 李冬梅,李志阳,杨君,等. 簕菜提取物对酒精性肝损伤大鼠的保护作用[J]. 食品工业科技,2021,42(18):380−386. doi:  10.13386/j.issn1002-0306.2021010238
LI Dongmei, LI Zhiyang, YANG Jun, et al. Protective Effect of Acanthopanax trifoliatus Extract on Rats with Alcoholic Liver Injury[J]. Science and Technology of Food Industry, 2021, 42(18): 380−386. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2021010238
Citation: LI Dongmei, LI Zhiyang, YANG Jun, et al. Protective Effect of Acanthopanax trifoliatus Extract on Rats with Alcoholic Liver Injury[J]. Science and Technology of Food Industry, 2021, 42(18): 380−386. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2021010238

簕菜提取物对酒精性肝损伤大鼠的保护作用

doi: 10.13386/j.issn1002-0306.2021010238
基金项目: 广州市科技局科研项目(201904010283);广东省南亚热带休闲农业工程技术研究中心资助项目(粤科函产学研字[2017]1649号)
详细信息
    作者简介:

    李冬梅(1975−),女,硕士,副教授,研究方向:食品加工与安全,E-mail:1308216802@qq.com

    通讯作者:

    林春华(1966−),女,硕士,教授,研究方向:特色蔬菜种质资源创新利用与休闲农业,E-mail:442612534@qq.com

  • 中图分类号: TS201.4

Protective Effect of Acanthopanax trifoliatus Extract on Rats with Alcoholic Liver Injury

  • 摘要: 目的:探讨簕菜不同提取物的解酒护肝作用,为簕菜解酒护肝功能食品的研发提供依据。方法:建立白酒灌胃大鼠致酒精肝损伤模型,考察簕菜醇提物和水提物对大鼠酒精耐受、睡眠和醒酒时间的影响,检测大鼠血液中乙醇质量浓度、血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)活性,以及大鼠肝脏组织中乙醇脱氢酶(ADH)、乙醛脱氢酶(ALDH)、超氧化物歧化酶(SOD)活性及谷胱甘肽(GSH)、丙二醛(MDA)含量,切片镜检肝细胞形态变化。结果:与酒精模型组比较,除水提物低剂量组对醉酒时间无显著变化(P>0.05)外,簕菜提取物各剂量组均可显著延长酒精耐受时间,并缩短睡眠、醒酒时间(P<0.05,P<0.01);簕菜提取物均可极显著降低血清中乙醇含量(P<0.01);除水提低剂量组AST活力无显著变化外(P>0.05),簕菜提取物各剂量组均能显著抑制酒精引起的大鼠血清ALT和AST活性升高(P<0.05);极显著增加肝脏组织中ADH、ALDH、SOD活力和GSH含量(P<0.01),降低MDA含量(P<0.01);肝细胞镜检结果显示,簕菜提取物均能在一定程度上减轻酒精导致的肝细胞变性程度。结论:簕菜醇提物、水提物均具有较好的防醉酒和解酒护肝作用,比较而言,醇提物效果优于水提物。
  • 图  1  簕菜提取物对急性酒精中毒大鼠肝脏病理形态学变化的影响(HE, 400×)

    Figure  1.  Effects of Acanthopanax trifoliatus extract on the pathological changes of liver in rats with acute alcoholism (HE, 400×)

    注:A.空白组;B.酒精模型组;C.阳性对照组;D.簕菜醇提低剂量组;E.簕菜醇提中剂量组;F.簕菜醇提高剂量组;G.簕菜水提低剂量组;H.簕菜水提中剂量组;I.簕菜水提高剂量组。

    表  1  簕菜提取物对大鼠酒精耐受、睡眠和醒酒时间的影响($\bar {\rm{x}} \pm {\rm{s}}$,n=7)

    Table  1.   Effects of Acanthopanax trifoliatus extract on the drunkenness, sleep and sobering times of SD rats ($\bar {\rm{x}} \pm {\rm{s}}$, n=7)

    组别剂量(g/kg)酒精耐受时间(min)睡眠时间(min)醒酒时间(min)
    酒精模型组19.13±1.74168.87±4.74188.00±5.89
    阳性对照组0.2732.59±2.29##122.70±7.97##155.29±6.78##
    簕菜醇提物低剂量组0.420.86±1.64##147.46±5.40##168.29±6.32##
    簕菜醇提物中剂量组0.829.03±2.12##131.03±8.34##159.29±6.99##
    簕菜醇提物高剂量组1.628.50±2.01##132.36±5.11##160.86±5.37##
    簕菜水提物低剂量组0.420.06±1.77151.94±5.32##172.00±5.63##
    簕菜水提物中剂量组0.828.63±2.08##130.94±4.61##159.57±5.00##
    簕菜水提物高剂量组1.620.53±1.63#143.47±3.97##164.00±3.06##
    注:与模型组比较,#表示差异显著,P<0.05,##表示差异极显著,P<0.01;表2同。
    下载: 导出CSV

    表  2  簕菜提取物对大鼠血液中乙醇含量的影响($\bar {\rm{x}} \pm {\rm{s}} $,n=7)

    Table  2.   Effects of Acanthopanax trifoliatus extract on the ethanol content in rat blood ($\bar {\rm{x}} \pm {\rm{s}} $, n=7)

    组别
    乙醇(g/L)
    0.5 h1 h2 h4 h
    酒精模型组4.58±0.084.55±0.124.52±0.074.39±0.21
    阳性对照组3.05±0.03##2.94±0.08##2.79±0.09##2.57±0.06##
    簕菜醇提物低剂量组3.70±0.12##3.63±0.15##3.68±0.08##3.59±0.07##
    簕菜醇提物中剂量组3.40±0.06##3.36±0.07##3.40±0.10##3.27±0.07##
    簕菜醇提物高剂量组3.07±0.08##2.97±0.11##2.91±0.10##2.82±0.06##
    簕菜水提物低剂量组3.87±0.07##4.05±0.21##3.82±0.21##3.75±0.07##
    簕菜水提物中剂量组3.45±0.06##3.41±0.11##3.36±0.06##3.30±0.13##
    簕菜水提物高剂量组3.17±0.13##3.14±0.13##3.03±0.10##2.96±0.08##
    下载: 导出CSV

    表  3  簕菜提取物对大鼠血清中ALT和AST活力的影响($\bar {\rm{x}} \pm {\rm{s}} $,n=7)

    Table  3.   Effects of Acanthopanax trifoliatus extract on the activities of ALT and AST in rat serum ($\bar {\rm{x}} \pm {\rm{s}} $, n=7)

    组别剂量(g·kg−1ALT(U/L)AST(U/L)
    空白组12.99±2.8495.84±4.76
    酒精模型组103.58±7.78**160.27±7.73**
    阳性对照组0.2713.57±2.32**##94.24±5.17**##
    簕菜醇提物低剂量组0.450.02±2.85**##99.38±8.14##
    簕菜醇提物中剂量组0.840.85±3.05**##79.53±4.40**##
    簕菜醇提物高剂量组1.624.50±2.83**##71.80±2.36**##
    簕菜水提物低剂量组0.484.25±2.66**##154.00±3.19**
    簕菜水提物中剂量组0.844.90±3.26**##134.62±7.73**#
    簕菜水提物高剂量组1.617.21±4.47**##84.97±5.07**##
    注:与空白组比较,*表示差异显著,P<0.05,**表示差异极显著,P<0.01;与模型组比较,#表示差异显著,P<0.05,##表示差异极显著,P<0.01;表4~表5同。
    下载: 导出CSV

    表  4  簕菜提取物对大鼠肝组织ADH、ALDH活性的影响($\bar {\rm{x}} \pm {\rm{s}} $,n=7)

    Table  4.   Effects of Acanthopanax trifoliatus extract on the activity of ADH, ALDH in rat liver tissue ($\bar {\rm{x}} \pm {\rm{s}} $, n=7)

    组别剂量(g·kg−1ADH(U/mg prot)ALDH(U/mg prot)
    空白组6.54±0.1120.05±0.41
    酒精模型组4.98±0.08**15.07±0.48**
    阳性对照组0.2710.00±0.44**##29.05±0.45**##
    簕菜醇提物低剂量组0.46.53±0.10##21.17±0.69*##
    簕菜醇提物中剂量组0.86.94±0.14**##21.40±0.46**##
    簕菜醇提物高剂量组1.67.05±0.19**##21.97±0.38**##
    簕菜水提物低剂量组0.46.63±0.12##21.52±0.52**##
    簕菜水提物中剂量组0.87.13±0.31**##22.81±0.43**##
    簕菜水提物高剂量组1.67.85±0.15**##25.79±0.39**##
    下载: 导出CSV

    表  5  簕菜提取物对大鼠肝组织GSH、MDA含量和SOD活性的影响($\bar {\rm{x}} \pm {\rm{s}} $,n=7)

    Table  5.   Effect of Acanthopanax trifoliatus extract on the contents of GSH, MDA and the activity of SOD in rats liver tissue ($\bar {\rm{x}} \pm {\rm{s}} $, n=7)

    组别剂量(g·kg−1GSH(μmol/g prot)MDA(μmol/g prot)SOD(U/mg prot)
    空白组13.24±0.293.96±0.04312.16±4.37
    酒精模型组8.77±0.42**5.69±0.04**281.33±4.20**
    阳性对照组0.2721.08±0.54**##3.73±0.02**##357.66±11.85**##
    簕菜醇提物低剂量组0.413.52±0.40 ##4.37±0.05**##330.17±3.38**##
    簕菜醇提物中剂量组0.815.35±0.53**##4.07±0.04**##336.07±2.53**##
    簕菜醇提物高剂量组1.614.77±0.20**##3.95±0.03##323.79±3.00**##
    簕菜水提物低剂量组0.413.95±0.45*##4.23±0.04**##320.37±2.13**##
    簕菜水提物中剂量组0.815.22±0.22**##4.27±0.03**##316.25±2.79*##
    簕菜水提物高剂量组1.615.13±0.23**##4.18±0.05**##311.15±1.69##
    下载: 导出CSV
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  • 收稿日期:  2021-02-01
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    《食品工业科技》编辑部携手万方数据开通学术不端专属检测通道,具体信息参见本刊动态。