鱼油对顺铂损伤HEK293细胞及A549细胞的影响

杜月梅; 刘云帆; 秦菲; 高丽萍

doi:10.13386/j.issn1002-0306.2020120173

鱼油对顺铂损伤HEK293细胞及A549细胞的影响

doi: 10.13386/j.issn1002-0306.2020120173

杜月梅^{1, 2},
刘云帆^{1, 2},
秦菲^{1, 2, ,},
高丽萍^{1, 2}

1.
北京联合大学生物化学工程学院，北京 100023
2.
生物活性物质与功能食品北京市重点实验室，北京 100191

基金项目: 北京联合大学研究生资助项目；北京联合大学推进产教融合，提升实践应用能力项目。

详细信息

作者简介:
杜月梅（1995−），女，硕士，研究方向：生物活性物质对机体的生化作用，E-mail：104665526@qq.com

通讯作者:
秦菲（1977−），女，博士，副教授，研究方向：生物活性物质对机体的生化作用，E-mail：qinfei@buu.edu.cn

中图分类号: R151.2
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- 文章访问数: 84
- HTML全文浏览量: 14
- PDF下载量: 19
- 被引次数: 0
出版历程
- 收稿日期: 2020-12-18
- 网络出版日期: 2022-10-21
- 刊出日期: 2022-11-23

Effects of Fish Oil Combined with Cisplatin on HEK293 Cells and A549 Cells

DU Yuemei^{1, 2},
LIU Yunfan^{1, 2},
QIN Fei^{1, 2
, ,},
GAO Liping^{1, 2}

1.
College of Biochemical Engineering, Beijing Union University, Beijing 100023, China
2.
Beijing Municipal Key Laboratory of Biologically Active Substances and Functional Food, Beijing 100191, China

摘要

摘要: 目的：探讨鱼油对顺铂（cis-dichlorodiamineplatinum (Ⅱ)，CDDP）抗A549人肺腺癌细胞活性的影响及对CDDP损伤HEK293细胞的保护作用。方法：体外培养A549和HEK293细胞，将细胞分为对照组、CDDP组、鱼油组、鱼油+CDDP组；分别用不同浓度的鱼油对细胞进行预处理，同时建立CDDP组、鱼油+CDDP组CDDP 损伤模型，CCK-8法检测细胞活力，试剂盒测定HEK293细胞中丙二醛(MDA)含量，二还原型谷胱甘肽(GSH)含量，超氧化物歧化酶(SOD)的活力等指标。结果：4~16 mg/L浓度的鱼油能够显著抑制CDDP造成的人胚肾HEK293细胞毒性（P<0.05），其中4 mg/L浓度的鱼油保护效果最佳，且此浓度的鱼油能够显著抑制CDDP（20 mg/L）引起的GSH含量降低以及MDA含量升高（P<0.05）。当鱼油浓度≥32 mg/L时显著增强了CDDP对A549细胞的抑制效果（P<0.05）。结论：一定浓度范围内，鱼油对CDDP致人胚肾细胞HEK293损伤具有一定的保护作用。低浓度鱼油对CDDP 所致A549细胞的抑制作用没有明显的影响，高浓度的鱼油增强了CDDP 的抗癌效果。
- 鱼油 /
- 顺铂 /
- 肾毒性 /
- 抗氧化 /
- 抗肿瘤
Abstract: Objective: To explore the effect of fish oil on the activity of cis-dichlorodiamineplatinum (Ⅱ) (CDDP) against A549 human lung adenocarcinoma cells and its protective effect on HEK293 cells damaged by CDDP. Methods: A549 and HEK293 cells were cultured in vitro, and the cells were divided into control group, CDDP group, fish oil group, fish oil+CDDP group; the cells were pretreated with different concentrations of fish oil, and CDDP injury models of CDDP group and fish oil+CDDP group were established at the same time. Cell viability was detected by CCK-8 method, and the kits were used to measure malondialdehyde (MDA) content, direduced glutathione (GSH) content, and superoxide dismutase (SOD) activity in HEK293 cells. Results: Fish oil at a concentration of 4~16 mg/L could significantly inhibit the cytotoxicity of HEK293 cells caused by CDDP (P<0.05). Fish oil at a concentration of 4 mg/L had the best protective effect, and fish oil at this concentration could significantly inhibit CDDP (20 mg/L) decreased GSH content and increased MDA content (P<0.05). When the fish oil concentration was ≥32 mg/L, the inhibitory effect of CDDP on A549 cells was significantly enhanced (P<0.05). Conclusion: Within a certain concentration range, fish oil has a certain protective effect on CDDP-induced damage to HEK293 of human embryonic kidney cells. Low-concentration fish oil has no obvious effect on the inhibitory effect of CDDP-induced A549 cells, and high-concentration fish oil enhances the anti-cancer effect of CDDP.
- fish oil /
- cisplatin /
- nephrotoxicity /
- anti-oxidant /
- antitumor

HTML全文

图 1 CDDP对HEK293细胞的毒性作用（n=6）

Figure 1. Toxic effect of CDDP on HEK293 cells (n=6)

注：与对照组比较，*表示P<0.05；图2、图4~图5同。

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图 2 不同浓度鱼油对HEK293 细胞存活率的影响（n=6）

Figure 2. Effects of different concentrations of fish oil on the survival rate of HEK293 cells (n=6)

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图 3 不同浓度鱼油对CDDP诱导HEK293细胞损伤的影响（n=6）

Figure 3. The effect of different concentrations of fish oil on the damage of HEK293 cells induced by CDDP (n=6)

注：与对照组相比，#表示P<0.05；与CDDP 模型组相比，*表示P<0.05；图6同。

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图 4 CDDP 对A549细胞的抑制作用（n=6）

Figure 4. Inhibition effect of CDDP on A549 cells (n=6)

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图 5 不同浓度鱼油对A549细胞存活率的影响（n=6）

Figure 5. Effects of different concentrations of fish oil on survival rate of A549 cells (n=6)

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图 6 不同浓度的鱼油联合CDDP 对A549 细胞的影响（n=6）

Figure 6. The effect of different concentrations of fish oil combined with CDDP on A549 cells (n=6)

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表 1 鱼油和顺铂联合用药对HEK293 细胞内抗氧化指标的影响

Table 1. The effect of the combination of fish oil and cisplatin on the anti-oxidation indexes in HEK293 cells

组别	SOD活力（U/mg pro）	GSH含量（μmol/g pro）	MDA含量（μmol/g pro）
对照组	45.94±1.21^b	72.89±2.39^a	1.03±0.02^c
CDDP组	36.38±2.44^c	45.98±1.11^c	1.54±0.04^a
鱼油组	52.20±4.53^a	76.11±4.52^a	0.90±0.01^c
鱼油+CDDP组	37.05±0.98^c	56.43±3.24^b	1.26±0.01^b
注：同列上标小写字母不同表示有统计学差异（P<0.05）。

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