姜黄素超分子包合物对乙醇诱导LO2细胞损伤的保护作用

范土贵 陈建平 高加龙 钟赛意 秦小明

范土贵,陈建平,高加龙,等. 姜黄素超分子包合物对乙醇诱导LO2细胞损伤的保护作用[J]. 食品工业科技,2021,42(18):366−371. doi:  10.13386/j.issn1002-0306.2020120083
引用本文: 范土贵,陈建平,高加龙,等. 姜黄素超分子包合物对乙醇诱导LO2细胞损伤的保护作用[J]. 食品工业科技,2021,42(18):366−371. doi:  10.13386/j.issn1002-0306.2020120083
FAN Tugui, CHEN Jianping, GAO Jialong, et al. Protective Effect of Curcumin/Cyclodextrin Polymer Inclusion Complex on LO2 Cells Damaged by Ethanol[J]. Science and Technology of Food Industry, 2021, 42(18): 366−371. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2020120083
Citation: FAN Tugui, CHEN Jianping, GAO Jialong, et al. Protective Effect of Curcumin/Cyclodextrin Polymer Inclusion Complex on LO2 Cells Damaged by Ethanol[J]. Science and Technology of Food Industry, 2021, 42(18): 366−371. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2020120083

姜黄素超分子包合物对乙醇诱导LO2细胞损伤的保护作用

doi: 10.13386/j.issn1002-0306.2020120083
基金项目: 广东省自然科学基金面上项目(2020A1515010860,2021A1515012455);广东海洋大学创新强校项目(230419100);广东海洋大学“南海学者计划”项目(002029002009)
详细信息
    作者简介:

    范土贵(1994−),男,硕士研究生,研究方向:天然产物活性物质及其生物利用,E-mail:ftg2693982106@163.com

    通讯作者:

    陈建平(1986−),男,博士,副教授,研究方向:天然产物活性物质及其生物利用,E-mail:cjp516555989@126.com

  • 中图分类号: TS201.2

Protective Effect of Curcumin/Cyclodextrin Polymer Inclusion Complex on LO2 Cells Damaged by Ethanol

  • 摘要: 本文考察了姜黄素超分子包合物(Curcumin/Cyclodextrin polymer inclusion complex, CUR/CDP)对乙醇诱导LO2细胞损伤的保护作用。采用MTT法建立乙醇诱导LO2细胞损伤的模型,通过谷氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、丙二醛(MDA)和活性氧(ROS)等试剂盒考察了CUR/CDP对乙醇诱导LO2细胞损伤的保护效果。结果表明,经CUR/CDP(80 μg/mL)处理后,LO2细胞的存活率从54.75%±8.97%(模型对照组)提高到85.27%±2.64%,且细胞培养液中ALT、AST和LDH活力分别从(26.47±0.90)、(41.02±4.41)、(63.77±4.95)U/L(模型对照组)降低到(16.17±0.42)、(22.62±0.79)、(32.25±1.69)U/L(P<0.01),LO2细胞内GSH-PX、SOD活力分别从(21.82±1.34)、(8.45±1.11)U/mg prot(模型对照组)升高到(36.70±0.56)、(16.47±1.27)U/mg prot,LO2细胞内MDA和ROS含量分别从(1.19±0.15)nmol/mg prot、(198.02%±11.76%)(模型对照组)降低到(0.72±0.05)nmol/mg prot、(110.87%±10.22%)(P<0.01)。综上所述,CUR/CDP通过提高LO2细胞相关抗氧化酶的活力和降低细胞内ROS含量来改善乙醇诱导LO2细胞的损伤。
  • 图  1  不同浓度的乙醇对LO2细胞存活率的影响

    Figure  1.  Effects of different ethanol concentrations on the cell viability of LO2 cells

    注:与对照组比较,*表示P<0.05,**表示P<0.01;500 mmol/L乙醇组与600 mmol/L乙醇组比较,#表示P<0.05。

    图  2  CUR/CDP对乙醇损伤LO2细胞存活率的影响

    Figure  2.  Effects of CUR/CDP on the cell viability of LO2 cells injured by ethanol

    注:与对照组比较,##表示P<0.01;与模型组比较,*表示P<0.05,**表示P<0.01;图3同。

    图  3  CUR/CDP对乙醇诱导LO2细胞损伤后ROS含量的影响

    Figure  3.  Effects of CUR/CDP on ROS content in LO2 cells damaged by ethanol

    表  1  CUR/CDP对乙醇诱导LO2细胞损伤后ALT、AST和LDH活力的影响

    Table  1.   Effects of CUR/CDP on the activities of ALT, AST and LDH in the culture medium of LO2 cells damaged by ethanol

    组别ALT(U/L)AST(U/L)LDH(U/L)
    阴性对照组14.63±1.5619.77±2.2727.91±5.08
    模型对照组26.47±0.90##41.02±4.41##63.77±4.95##
    10 µg/mL CUR/CDP23.66±1.5935.01±1.6954.74±5.99
    20 µg/mL CUR/CDP21.96±1.80*30.76±2.08*50.68±2.61*
    40 µg/mL CUR/CDP18.58±0.23**24.93±1.20**42.01±2.15**
    80 µg/mL CUR/CDP16.17±0.42**22.62±0.79**32.25±1.69**
    注:与阴性对照组比较,##表示P<0.01;与模型对照组比较,*表示P<0.05,**表示P<0.01;表2同。
    下载: 导出CSV

    表  2  CUR/CDP对乙醇损伤LO2细胞中SOD活力、GSH-PX活力和MDA含量的影响

    Table  2.   Effects of CUR/CDP on SOD activity, GSH-PX activity and MDA contents in LO2 cells damaged by ethanol

    组别SOD(U/mg prot)GSH-PX(U/mg prot)MDA(nmol/mg prot)
    阴性对照组18.80±0.5139.29±1.120.64±0.13
    模型对照组8.45±1.11##21.82±1.34##1.19±0.15##
    10 µg/mL CUR/CDP10.36±0.3924.26±0.701.05±0.07
    20 µg/mL CUR/CDP12.58±1.08*28.31±2.10*0.96±0.10*
    40 µg/mL CUR/CDP15.05±0.31**33.76±1.20**0.81±0.09**
    80 µg/mL CUR/CDP16.47±1.27**36.70±0.56**0.72±0.05**
    下载: 导出CSV
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  • 收稿日期:  2020-12-09
  • 网络出版日期:  2021-08-05
  • 刊出日期:  2021-09-14

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