桦褐孔菌与其固体发酵物对2型糖尿病大鼠的氧化应激损伤作用

王欢 王丽莹 刘俊泽 唐敏 陈长宝 王淑敏

王欢,王丽莹,刘俊泽,等. 桦褐孔菌与其固体发酵物对2型糖尿病大鼠的氧化应激损伤作用[J]. 食品工业科技,2021,42(20):328−333. doi:  10.13386/j.issn1002-0306.2020090128
引用本文: 王欢,王丽莹,刘俊泽,等. 桦褐孔菌与其固体发酵物对2型糖尿病大鼠的氧化应激损伤作用[J]. 食品工业科技,2021,42(20):328−333. doi:  10.13386/j.issn1002-0306.2020090128
WANG Huan, WANG Liying, LIU Junze, et al. Effects of Sclerotium and Solid Fermentation Products of Inonotus obliquus on Inflammation and Oxidative Stress in Diabetic Rats[J]. Science and Technology of Food Industry, 2021, 42(20): 328−333. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2020090128
Citation: WANG Huan, WANG Liying, LIU Junze, et al. Effects of Sclerotium and Solid Fermentation Products of Inonotus obliquus on Inflammation and Oxidative Stress in Diabetic Rats[J]. Science and Technology of Food Industry, 2021, 42(20): 328−333. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2020090128

桦褐孔菌与其固体发酵物对2型糖尿病大鼠的氧化应激损伤作用

doi: 10.13386/j.issn1002-0306.2020090128
基金项目: 国家重点研发计划项目(2019YFC1710704);2021年吉林省科技发展计划项目(202522YY010680786)
详细信息
    作者简介:

    王欢(1987−),女,博士,助理研究员,研究方向:菌物药活性次生代谢产物,E-mail:wanghuanmyco@163.com

    通讯作者:

    陈长宝(1962−),男,博士,教授,研究方向:生药资源开发与利用,E-mail:ccb2021@126.com

    王淑敏(1965−),女,博士,教授,研究方向:中药生药学、微生物与生化药学,E-mail:wangsm@ccucm.edu.cn

  • 中图分类号: TS201.4

Effects of Sclerotium and Solid Fermentation Products of Inonotus obliquus on Inflammation and Oxidative Stress in Diabetic Rats

  • 摘要: 目的:研究比较桦褐孔菌菌核、固体发酵物和二者的水提物给药对糖尿病大鼠机体炎症和氧化应激损伤的作用。方法:本研究采用高糖高脂饮食联合STZ诱导的2型糖尿病大鼠模型,监测大鼠体重、口服糖耐量试验、酶联免疫吸附试验(ELISA)检测大鼠血清中炎症因子白细胞介素IL-6、IL-1β,肿瘤坏死因子TNF-α、超氧化物歧化酶SOD及丙二醛MDA的含量。结果:与模型组相比,桦褐孔菌IO 3.08、6.16 g/kg剂量组、桦褐孔菌水提物WIO 0.4 g/kg剂量组、桦褐孔菌固体发酵物水提物WSFIO 0.8 g/kg剂量组均可显著提高糖尿病大鼠体重;固体发酵物SFIO 1.3、2.6 g/kg剂量组能显著地改善大鼠口服糖耐量(P<0.05,P<0.01);WIO 0.4、0.8 g/kg剂量组、WSFIO 0.4 g/kg剂量组均可显著降低血清中IL-1β的水平;IO 3.08、6.16 g/kg剂量组、WIO 0.4 g/kg剂量组、SFIO 1.3、2.6 g/kg剂量组和WSFIO 0.8 g/kg剂量组均可显著降低血清中TNF-α的水平;桦褐孔菌各给药组在不同程度上均可显著提高血清中SOD的水平(P<0.05,P<0.01或P<0.001);桦褐孔菌各给药组均可降低血清中MDA的水平(P<0.01或P<0.001)。结论:桦褐孔菌固体发酵物具有良好的抗炎及抗氧化活性。直接以菌粉、固体发酵物或二者的水提物给药对各个指标的影响不一,总体来说,固体发酵物代替野生桦褐孔菌改善2型糖尿病的炎症及氧化应激具有可行性。
  • 表  1  桦褐孔菌对糖尿病大鼠体重的影响(${\rm{\bar x}}$±s, g, n=10)

    Table  1.   Effects of I. obliquus on body weight of the diabetic rats (${\rm{\bar x}}$±s, g, n=10)

    时间(周)空白组模型组阳性药组IO组(g/kg) WIO组(g/kg)
    3.086.160.40.8
    0212.4±11.8222.3±4.8221.9±13.1226.0±5.7218.8±13.5218.3±10.1221.5±11.3
    1283.7±11.1283.0±16.3276.7±12.5288.5±13.6286.3±11.7282.3±15.2286.1±22.3
    2346.4±13.6332.3±35.6323.1±16.4340.4±16.4340.6±12.8340.8±12.5343.5±30.6
    3384.6±14.4365.7±44.2352.9±21.4383.0±22.4390.3±13.8383.4±19.1380.7±38.1
    4418.9±13.8390.0±55.4376.4±23.2417.0±28.3414.9±14.6408.4±17.3410.7±42.6
    5462.4±15.7412.5±40.0###396.8±22.1444.5±23.01453.1±26.6*442.2±21.2424.5±32.4
    6505.8±25.7430.5±44.9###401.8±28.1448.3±27.0457.6±34.6444.2±24.9437.9±33.8
    7529.5±16.7426.0±61.9###405.6±31.5461.4±13.2*470.4±27.0**458.5±34.5438.9±15.2
    8529.9±18.6433.0±57.0###411.3±32.2472.3±11.2*488.2±29.0***467.0±40.1443.9±26.7
    9545.6±10.7435.8±58.1###414.7±27.3476.7±11.3*487.0±34.3**467.0±41.1448.8±10.6
    10554.5±14.9433.0±56.3###415.3±37.0485.3±12.9***487.8±37.9***475.9±55.3**450.2±13.9
    11562.8±17.4430.2±53.0###414.3±36.3480.6±13.5**487.1±40.5***471.0±37.3*439.7±9.9
    12572.2±19.0417.6±43.7###405.2±39.2472.3±20.5***478.3±41.3***462.7±39.3**432.2±15.8
    13578.9±21.5413.8±37.2###405.7±41.7456.4±23.7**474.0±46.2***457.6±43.0**430.2±13.4
    14599.0±20.3386.4±36.4###383.3±50.1434.8±41.3**449.4±49.8***425.7±48.8*401.7±13.6
    下载: 导出CSV
    时间(周)SFIO组(g/kg) WSFIO组(g/kg)
    1.32.60.40.8
    0217.5±12.2212.6±6.8218.3±5.9232.4±4.9
    1283.9±8.0286.3±7.5336.4±10.5288.2±15.7
    2321.7±26.3346.4±5.9379.5±16.8341.8±16.4
    3359.1±32.8383.7±9.0410.9±20.0385.0±19.0
    4384.6±43.2404.6±12.6448.8±28.8406.5±25.9
    5404.3±44.0405.2±20.6457.3±21.4434.6±21.4
    6417.4±41.1426.8±26.5460.2±22.4453.3±18.8
    7421.2±40.7428.7±37.3467.1±32.3461.2±22.0
    8424.5±39.0429.9±35.8459.6±32.7469.0±17.4
    9439.9±42.3437.1±42.7464.8±29.6472.0±17.7*
    10440.6±33.5442.2±45.0461.7±32.3473.5±16.0*
    11439.5±34.7444.0±40.5447.5±35.9471.8±13.0*
    12438.2±32.2432.1±47.8434.3±43.0467.9±12.1**
    13429.5±28.8416.8±54.0429.5±45.3458.0±30.4**
    14403.6±29.5403.7±56.0409.9±43.2444.3±22.9***
    注:与空白组比较:#P<0.05, ##P<0.01, ###P<0.001;与模型组比较,*P<0.05, **P<0.01, ***P<0.001;表2~表4同。
    下载: 导出CSV

    表  2  桦褐孔菌对糖尿病大鼠口服糖耐量的影响(${\rm{\bar x}}$±s, mmol/L, n=10)

    Table  2.   Effects of I. obliquus on oral glucose tolerance of the diabetic rats (${\rm{\bar x}}$±s, mmol/L, n=10)

    组别剂量(g/kg)0 min30 min60 min90 min120 min
    空白组4.13±1.136.51±0.626.53±0.305.53±1.235.48±0.60
    模型组11.77±4.91##25.23±2.41###21.17±4.80###20.77±6.10###17.87±5.74###
    阳性药组0.28.35±2.2722.83±4.1622.00±5.8318.25±4.9515.07±5.29
    IO组3.088.28±2.5923.74±4.3316.36±6.8217.46±5.7214.74±6.79
    IO组6.167.97±0.2123.07±1.2422.94±4.1520.13±7.5115.63±6.75
    WIO组0.47.80±0.9623.90±3.3819.73±4.1418.60±2.8016.10±1.47
    WIO 组0.810.25±6.5823.50±1.2719.60±0.7119.15±1.2014.90±0.92
    SFIO 组1.38.70±0.6017.90±8.63**17.80±6.4715.50±9.1913.70±9.97
    SFIO 组2.68.03±1.7218.83±0.46*20.97±3.2117.97±2.8114.64±1.85
    WSFIO 组0.48.37±0.1521.10±3.3821.07±1.1418.27±2.8715.10±3.59
    WSFIO 组0.810.48±4.9221.10±3.0819.23±1.2316.13±1.0512.55±0.89
    下载: 导出CSV

    表  3  桦褐孔菌对糖尿病大鼠血清中IL-6,IL-1β,TNF-α水平的影响(${\rm{\bar x}}$±s,n=10)

    Table  3.   Effects of I. obliquus on IL-6,IL-1β and TNF-α levels in serum of the diabetic rats (${\rm{\bar x}}$±s, n=10)

    组别剂量(g/kg)IL-6(pg/mL)IL-1β(pg/mL)TNF-α(pg/mL)
    空白组92.18±19.4897.07±4.0871.36±5.59
    模型组190.55±28.24##141.27±37.76#91.46±13.66##
    阳性药组0.2122.63±25.02*83.11±14.24**75.65±4.60**
    IO 组3.08182.48±18.45138.84±8.9470.88±6.85***
    IO 组6.16177.32±9.96141.31±19.3270.97±17.01***
    WIO 组0.4192.57±25.3182.06±8.23**77.23±7.72*
    WIO 组0.8127.83±24.9884.63±13.37*80.41±3.10
    SFIO 组1.3175.82±10.80143.28±11.9075.80±8.44**
    SFIO 组2.6145.46±11.09144.64±11.9369.71±12.06***
    WSFIO 组0.4148.29±32.9884.23±42.66*92.69±1.72
    WSFIO 组0.8138.76±48.02154.50±2.6165.41±13.58***
    下载: 导出CSV

    表  4  桦褐孔菌对糖尿病大鼠血清中SOD和MDA水平的影响(${\rm{\bar x}}$±s,n=10)

    Table  4.   Effects of I. obliquus on SOD and MDA levels in serum of the diabetic rats

    组别剂量(g/kg)SOD(U/mL)MDA(mmol/mL)
    空白组85.24±1.0811.35±1.23
    模型组51.22±1.56###15.22±2.11###
    阳性组0.279.88±2.02***10.66±2.58***
    IO 组3.0868.56±2.13***10.14±1.27***
    IO 组6.1672.33±1.98***9.65±0.63***
    WIO 组0.453.47±1.66*8.87±1.10***
    WIO 组0.853.95±1.40**8.12±1.47***
    SFIO 组1.370.02±2.45***11.76±0.75**
    SFIO 组2.675.66±2.00***11.76±1.09**
    WSFIO 组0.466.81±1.87***9.21±1.69***
    WSFIO 组0.870.74±1.61***11.24±1.27***
    下载: 导出CSV
  • [1] Duru KC, Kovaleva E G, Danilova I G, et al. The pharmacological potential and possible molecular mechanisms of action of Inonotus obliquus from preclinical studies[J]. Phytotherapy Research,2019,33(8):1966−1980. doi:  10.1002/ptr.6384
    [2] Choi S Y, Hur S J, An C H, et al. Anti-inflammatory effects of Inonotus obliquus in colitis induced by dextran sodium sulfate[J]. Journal of Biomedicine & Biotechnology,2010:943516−943515. doi:  10.1155/2010/943516
    [3] Zhao F Q, Mai Q Q, Ma J H, et al. Triterpenoids from Inonotus obliquus and their antitumor activities[J]. Fitoterapia,2015,101:34−40. doi:  10.1016/j.fitote.2014.12.005
    [4] Blagodatski A, Yatsunskaya M, Mikhailova V, et al. Medicinal mushrooms as an attractive new source of natural compounds for future cancer therapy[J]. Oncotarget,2018,9:29259−29274. doi:  10.18632/oncotarget.25660
    [5] 张树庭, Solomon P Wasser. 药用菌及其产品在农业和生物医学领域中的应用现状与未来研究趋势(下)[J]. 食药用菌,2019,27(3):149−155. [Zhang S T, Solomon P Wasser. Application status and future research trend of medicinal fungi and their products in agriculture and biomedical field[J]. Edible and Medicinal Mushrooms,2019,27(3):149−155.
    [6] 牛君, 王傲, 史钏, 等. 中国食用菌降血糖研究进展[J]. 中国食用菌,2020,39(1):1−7, 15. [Niu J, Wang A, Shi C, et al. Advance research on hypoglycemic effect of edible fungi in China[J]. Edible Fungi of China,2020,39(1):1−7, 15.
    [7] 杜文婧, 王琦. 桦褐孔菌资源分布及药理活性研究进展[J]. 菌物研究,2013,11(1):49−56. [Du W J, Wang Q. A review of the distribution and pharmacological activity of Inonotus obliquus[J]. Journal of Fungal Research,2013,11(1):49−56.
    [8] Kim Y O, Park H W, Kim J H, et al. Anti-cancer effect and structural characterization of endopolysaccharide from cultivated mycelia of Inonotus obliquus[J]. Life Sciences,2006,79:72−80. doi:  10.1016/j.lfs.2005.12.047
    [9] Saar M. Fungi in Khanty folk medicine[J]. Journal of Ethnopharmacology,1991,31(2):175−179. doi:  10.1016/0378-8741(91)90003-V
    [10] 马丹丹, 汪雯翰, 薛蓓, 等. 桦褐孔菌降糖活性成分及治疗糖尿病机制研究进展[J]. 南京师大学报(自然科学版),2019,42(4):111−117. [Ma D D, Wang W H, Xue B, et al. Hypoglycemic active components and treatment of Inonotus obliquus advances in research on mechanism of diabetes[J]. Journal of Nanjing Normal University (Natural Science Edition),2019,42(4):111−117.
    [11] Zhang N, Chen H, Ma L, et al. Physical modifications of polysaccharide from Inonotus obliquus and the antioxidant properties[J]. Int. J. Biol. Macromol,2013,54:209−215. doi:  10.1016/j.ijbiomac.2012.12.030
    [12] Wang C, Chen Z Q, Pan Y X, et al. Anti-diabetic effects of Inonotus obliquus polysaccharides-chromium (III) complex in type 2 diabetic mice and its sub-acute toxicity evaluation in normal mice[J]. Food and Chemical Toxicology,2017:498−509.
    [13] Sun J E, Ao Z H, Lu Z M, et al. Antihyperglycemicn and antilipidperoxidative effects of dry matter of culture broth of Inonotus obliquus in submerged culture on normal and alloxan-diabetes mice[J]. Journal of Ethnopharmacology,2008,118:7−13. doi:  10.1016/j.jep.2008.02.030
    [14] Lemieszek M K, Langner E, Kaczor J, et al. Anticancer effects of fraction isolated from fruiting bodies of Chaga medicinal mushroom, Inonotus obliquus (Pers. : Fr.) Pilát (Aphyllophoromycetideae): in vitro studies[J]. International Journal of Medicinal Mushrooms,2011,162(2):131−143.
    [15] Glamočlija J, Ćirić A, Nikolić M, et al. Chemical characterization and biological activity of Chaga (Inonotus obliquus), a medicinal “mushroom”[J]. Journal of Ethnopharmacology,2015,162:323−332. doi:  10.1016/j.jep.2014.12.069
    [16] Kim H G, Yoon D H, Kim C H, et al. Ethanol extract of Inonotus obliquus inhibits lipopolysaccharide-induced inflammation in RAW 264.7 macrophage cells[J]. Journal of Medicinal Food,2007,10:80−89. doi:  10.1089/jmf.2006.156
    [17] Shashkina M Y, Shashkin P N, Sergeev A V. Chemical and medicobiological properties of chaga (review)[J]. Pharmaceut Chem J,2006,40:560−568. doi:  10.1007/s11094-006-0194-4
    [18] Konrad AS, Bartosz S, Tadeusz P, et al. Inonotus obliquus-from folk medicine to clinical use[J]. Journal of Traditional and Complementary Medicine,2021,11(4):293−302. doi:  10.1016/j.jtcme.2020.08.003
    [19] Zhang C J, Guo J Y, Cheng H, et al. Spatial structure and anti-fatigue of polysaccharide from Inonotus obliquus[J]. Int J Biol Macromol,2020,151:855e860.
    [20] Liu Z, Yu D, Li L, et al. Three-phase partitioning for the extraction and purification of polysaccharides from the immunomodulatory medicinal mushroom Inonotus obliquus[J]. Molecules,2019,24(3):1e14.
    [21] Nguyet T M N, Lomunova M, Le BV, et al. The mast cell stabilizing activity of Chaga mushroom critical for its therapeutic effect on food allergy is derived from inotodiol[J]. Int Immunopharm,2017,2018(54):286e295.
    [22] 史晓虎. 通过抑制微炎症, 干预糖尿病肾病的分子机制初探[D]. 北京: 北京协和医学院, 2014.

    Shi X H. New treatment strategy on diabetic kidney disease by targeting to inflammation[D]. Beijing: Chinese Academy of Medical Sciences & Peking Union Medical College, 2014.
    [23] 常化静, 何羡霞, 苏楠, 等. IKK/NF-κB信号通路与2型糖尿病及中药抗炎治疗[J]. 中华中医药学刊,2014,32(8):1952−1955. [Chang H J, He X X, Su N, et al. IKK/NF-κB signal pathway and type 2 diabetes and anti-inflammatory treatment of traditional Chinese medicine[J]. Chinese Archives of Traditional Chinese Medicine,2014,32(8):1952−1955.
    [24] 戚琛晔. 2型糖尿病大鼠氧化应激与骨骼肌细胞GLUT4表达及转位的相关性研究[D]. 河南: 河南科技大学, 2014.

    Qi C Y. Correlation study between oxidative stress with GLUT4 expression and translocation in type 2 diabetic rat skeletal muscle cells[D]. Henan: Henan University of Science and Technology, 2014.
    [25] 刘迪. 人参皂苷协同抑制HEK-293细胞氧化应激损伤及机制研究[D]. 长春: 吉林大学, 2016.

    Liu D. Study on synergistic effects and mechanisms of ginsenoside against oxidative stress in human embryonic kidney 293 cells[D]. Changchun: Jilin University, 2016.
    [26] Wang J, Wang C, Li S, et al. Anti-diabetic effects of Inonotus obliquus polysaccharides in streptozotocininduced type 2 diabetic mice and potential mechanism via PI3K-Akt signal pathway[J]. Biomedicine & Pharmacotherapy,2017,95:1669−1677.
    [27] 童楠. 益肾颗粒调控足细胞自噬改善糖尿病贤病的作用机制研究[D]. 北京: 中国中医科学院, 2018.

    Dong N. Effect and mechanism of regulating autophagy in podocyte to improve diabetic kidney disease with Yishen decoction[D]. Beijing: China Academy of Chinese Medical Science, 2018.
    [28] 王欢, 王淑敏, 陈长宝, 等. 黄绿卷毛菇水提物对糖尿病大鼠机体抗氧化及抗炎作用初探[J]. 菌物学报,2019,38(9):1519−1526. [Wang H, Wang S M, Chen C B, et al. Assessment of antioxidant and anti-inflammatory potential of the aqueous extract of Floccularia luteovirens in diabetic rats[J]. Mycosystema,2019,38(9):1519−1526.
    [29] 马春宇, 于洪宇, 王慧娇, 等. 苦瓜总皂苷对 2 型糖尿病大鼠降血糖作用机制的研究[J]. 天津医药,2014,42(4):321−324. [Ma C Y, Yu H Y, Wang H J, et al. Hypoglycemic mechanism of total saponins of Momordica charantia in type 2 diabetes mellitus rats[J]. Tianjin Med J,2014,42(4):321−324. doi:  10.3969/j.issn.0253-9896.2014.04.010
    [30] 张苗, 李建宽, 葛睿, 等. 桦褐孔菌总多糖抗糖尿病作用的研究[J]. 山西医科大学学报,2020,51(4):327−331. [Zhang M, Li J K, Ge R, et al. Antidiabetic effects of Inonotus obliquus total polysaccharides[J]. J Shanxi Med Univ,2020,51(4):327−331.
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  • 收稿日期:  2020-09-14
  • 网络出版日期:  2021-08-29
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