藤茶提取物的降尿酸作用研究

吴淑慧 於洪建 於天 郑志刚 柯达 赵玲

吴淑慧,於洪建,於天,等. 藤茶提取物的降尿酸作用研究[J]. 食品工业科技,2021,42(18):350−355. doi:  10.13386/j.issn1002-0306.2020080100
引用本文: 吴淑慧,於洪建,於天,等. 藤茶提取物的降尿酸作用研究[J]. 食品工业科技,2021,42(18):350−355. doi:  10.13386/j.issn1002-0306.2020080100
WU Shuhui, YU Hongjian, YU Tian, et al. Study on Anti-hyperuricemia Effect of Ampelopsis grossedentata Extracts[J]. Science and Technology of Food Industry, 2021, 42(18): 350−355. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2020080100
Citation: WU Shuhui, YU Hongjian, YU Tian, et al. Study on Anti-hyperuricemia Effect of Ampelopsis grossedentata Extracts[J]. Science and Technology of Food Industry, 2021, 42(18): 350−355. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2020080100

藤茶提取物的降尿酸作用研究

doi: 10.13386/j.issn1002-0306.2020080100
详细信息
    作者简介:

    吴淑慧(1994−),女,硕士研究生,研究方向:天然药物的开发与利用,E-mail:wushuhui1212@163.com

    通讯作者:

    赵玲(1982−),女,博士,副教授,研究方向:天然药物的开发与利用,E-mail:zhaolingcpu@126.com

  • 中图分类号: TS201.4

Study on Anti-hyperuricemia Effect of Ampelopsis grossedentata Extracts

  • 摘要: 目的:研究藤茶提取物对高尿酸血症小鼠的降尿酸效果和对肝肾的保护作用。方法:腹腔注射氧嗪酸钾诱导建立高尿酸血症小鼠模型,以藤茶提取物低、中、高剂量(100、200、400 mg/kg)和别嘌呤醇(10 mg/kg)连续灌胃14 d,检测小鼠血清尿酸(Uric acid, UA)、尿素氮(Blood urea nitrogen, BUN)、肌酐(Creatinine, CRE)、天门冬氨酸氨基转氨酶(Aspartate aminotransferase, AST)、谷氨酸氨基转氨酶(Alanine aminotransferase, ALT)和肝脏黄嘌呤氧化酶(Xanthine oxidase, XOD)水平;计算肝肾系数后切片染色,观察肝肾组织的病理学特征。结果:与模型组相比,200和400 mg/kg藤茶提取物组小鼠血清尿酸值分别降低22.65%(P<0.05)和29.15%(P<0.01),显著降低血清肌酐和尿素氮水平(P<0.05), 下调天门冬氨酸氨基转氨酶、谷氨酸氨基转氨酶和肝脏黄嘌呤氧化酶水平(P<0.05),降低肝肾系数;病理组织形态学检查结果发现藤茶提取物对高尿酸血症小鼠肝组织和肾组织有明显改善作用。结论:200、400 mg/kg藤茶提取物对高尿酸血症小鼠有良好的降尿酸效果和肝肾保护作用。
  • 图  1  小鼠体重相对变化率情况

    Figure  1.  Relative change rate of mice body weight

    图  2  藤茶提取物对高尿酸血症小鼠血清尿酸值的影响

    Figure  2.  Effects of Ampelopsis grossedentata extract on serum uric acid value in HUA mice

    注:与正常组比较,*:P<0.05,**:P<0.01;与模型组比较,#:P<0.05,##:P<0.01。图3~图5同。

    图  3  藤茶提取物对高尿酸血症小鼠血清肌酐和尿素氮含量的影响

    Figure  3.  Effects of Ampelopsis grossedentata extract on serum CRE and BUN in HUA mice

    图  4  藤茶提取物对高尿酸血症小鼠血清AST和AST含量的影响

    Figure  4.  Effects of Ampelopsis grossedentata extract on serum AST and ALT in HUA mice

    图  5  藤茶提取物对高尿酸血症小鼠肝脏组织XOD活性的影响

    Figure  5.  Effects of Ampelopsis grossedentata extract on XOD activities in HUA mice

    图  6  AGE对氧嗪酸钾诱导的高尿酸血症小鼠肾脏的影响(200×)

    Figure  6.  Effects of AGE on the influence of kidney in hyperuricemia mice induced by potassium oxonate(200×)

    注:正常组(A);模型组(B);别嘌醇组(C);高剂量组(D);中剂量组(E);低剂量组(F)。图7同。

    图  7  AGE对氧嗪酸钾诱导的高尿酸血症小鼠肝脏的影响(200×)

    Figure  7.  Effects of AGE on the influence of liver in hyperuricemia mice induced by potassium oxonate (200×)

    表  1  藤茶提取物对高尿酸血症小鼠脏器系数的影响

    Table  1.   Effects of Ampelopsis grossedentata extract on organ coefficient of HUA mice

    组别肝脏系数(%)肾脏系数(%)
    NC7.5907±0.852.0237±0.19
    MC7.9295±0.88*2.4987±0.27*
    AGEL7.9078±0.762.3199±0.14
    AGEM7.837±0.592.1427±0.36#
    AGEH7.6305±0.982.1287±0.11#
    ALLO7.4616±0.63#2.0291±0.25#
    注:与正常组比较,*:P<0.05,**:P<0.01;与模型组比较,#:P<0.05,##:P<0.01。
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  • 收稿日期:  2020-08-13
  • 网络出版日期:  2021-08-04
  • 刊出日期:  2021-09-14

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