响应面法优化黑胡萝卜红色素双水相萃取工艺

李治城 武玉倩 张婕妤 展亚莉 常秀莲

李治城,武玉倩,张婕妤,等. 响应面法优化黑胡萝卜红色素双水相萃取工艺[J]. 食品工业科技,2021,42(7):195−200. doi: 10.13386/j.issn1002-0306.2020060219
引用本文: 李治城,武玉倩,张婕妤,等. 响应面法优化黑胡萝卜红色素双水相萃取工艺[J]. 食品工业科技,2021,42(7):195−200. doi: 10.13386/ j.issn1002-0306.2020060219
LI Zhicheng, WU Yuqian, ZHANG Jieyu, et al. Optimization of Extraction Technology of Anthocyanidins from Daucus carota L. by Aqueous Two-phase System Based on Response Surface Methodology[J]. Science and Technology of Food Industry, 2021, 42(7): 195−200. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2020060219
Citation: LI Zhicheng, WU Yuqian, ZHANG Jieyu, et al. Optimization of Extraction Technology of Anthocyanidins from Daucus carota L. by Aqueous Two-phase System Based on Response Surface Methodology[J]. Science and Technology of Food Industry, 2021, 42(7): 195−200. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2020060219

响应面法优化黑胡萝卜红色素双水相萃取工艺

doi: 10.13386/j.issn1002-0306.2020060219
基金项目: 烟台大学研究生科技创新基金资助(YDZD2029);横向课题(SK18H08,SK18H09,SK19H21)
详细信息
    作者简介:

    李治城(1995−),男,硕士研究生,研究方向:天然产物分离,E-mail:Lizhicheng1208@163.com

    通讯作者:

    常秀莲(1965−),女,博士,教授,研究方向:天然产物分离,E-mail:changxiulian7@126.com

  • 中图分类号: TS202.3

Optimization of Extraction Technology of Anthocyanidins from Daucus carota L. by Aqueous Two-phase System Based on Response Surface Methodology

  • 摘要: 采用聚乙二醇(PEG)/硫酸铵双水相体系萃取纯化黑胡萝中的花青素。通过单因素实验考察了硫酸铵、PEG6000、粗提液的质量分数对花青素选择性系数和萃取率的影响,并以花青素的萃取率为响应值,结合响应面试验优化萃取工艺。结果表明,各因素对萃取率的影响程度由大到小依次为硫酸铵质量分数>粗提液质量分数>PEG6000质量分数。以双水相体系的总质量为基准,实验最佳萃取条件为:硫酸铵质量分数为20.0%、粗提液质量分数为12.0%、PEG6000质量分数为14.0%,黑胡萝卜红色素的平均萃取率为94.17%。此萃取方法得到的黑胡萝卜红色素具有一定的抗氧化活性,对DPPH自由基清除能力的IC50值为30.51 mg/L。
  • 图  1  硫酸铵的质量分数对选择性系数和萃取率的影响

    Figure  1.  Effect of mass fraction of ammonium sulfate on selectivity coefficient and extraction rate

    图  2  PEG6000的质量分数对选择性系数和萃取率的影响

    Figure  2.  Effect of mass fraction of PEG6000 on selectivity coefficient and extraction rate

    图  3  粗提液质量分数对选择性系数和萃取率的影响

    Figure  3.  Effect of mass fraction of crude extract on selectivity coefficient and extraction rate

    图  4  各因素相互作用响应面图

    Figure  4.  Response surface plot of the interaction between various factors

    图  5  黑胡萝卜红色素和VC的DPPH自由基清除能力

    Figure  5.  DPPH free radical scavenging ability of black carrot red color and VC

    表  1  响应面试验设计

    Table  1.   Design of response surface experiment

    水平因素
    A:(NH42SO4
    质量分数(%)
    B:PEG6000
    质量分数(%)
    C:粗提液质量
    分数(%)
    −1181210
    0201412
    1221614
    下载: 导出CSV

    表  2  响应面试验结果

    Table  2.   Results of response surface experiment

    试验号因素萃取率Y(%)
    ABC
    110−188.21
    2−1−1086.89
    3−11087.79
    410188.27
    51−1088.83
    60−1−191.17
    701−190.07
    800092.23
    9−10185.37
    1000095.47
    110−1189.51
    1200093.73
    1311088.62
    1400095.28
    15−10−186.73
    1600094.82
    1701189.24
    下载: 导出CSV

    表  3  响应面试验方差分析结果

    Table  3.   Results of analysis of variance of response surface experiment

    方差来源均方和自由度均方FP显著性
    模型149.17916.5714.660.0009**
    A7.1317.136.300.0404*
    B0.1510.150.130.7301
    C1.811.801.590.2480
    AB0.5710.570.50.5006
    AC0.510.500.450.5257
    BC0.1710.170.150.7079
    A285.76185.7675.85<0.0001**
    B211.61111.6110.270.0150*
    C229.52129.5226.110.0014**
    残差7.9171.13
    失拟项0.730.230.130.9371
    纯误差7.2141.8
    总离差157.0816
    注:*表示显著(P<0.05);**表示极显著(P<0.01)。
    下载: 导出CSV
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出版历程
  • 收稿日期:  2020-06-19
  • 网络出版日期:  2021-01-28
  • 刊出日期:  2021-04-01

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