Ultrasonic Extraction of β-Carotene from Spirulina platensis in Aqueous Two-phase System and Its Anti-glycosylation Effect
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摘要: 研究超声辅助有机溶剂/糖双水相体系萃取螺旋藻中β-胡萝卜素最佳提取条件，并对其抗糖基化作用进行分析，在确定萃取体系为叔丁醇/麦芽糖双水相体系基础上，以螺旋藻粉末加入量、超声时间、超声功率为自变量，β-胡萝卜素得率为因变量，采用正交试验设计优化萃取条件。采用赖氨酸-乳糖模拟体系评价萃取后β-胡萝卜素抗糖基化能力。结果表明：3.4 g叔丁醇/2.4 g麦芽糖体系中，螺旋藻粉末加入量为0.05 g，补足水分至10 g，在超声功率90 W，超声时间5 min条件下，螺旋藻中β-胡萝卜素萃取得率为3.19 mg/g，在浓度为50～450 μg/mL范围内，其对模拟体系形成的晚期糖基化终产物的最高抑制率为47.28%，可为新型晚期糖基化终末产物（AGEs）抑制剂开发提供参考依据。Abstract: The optimum extraction conditions of β-carotene from Spirulina platensis by ultrasonic assisted organic solvent / sugar aqueous two-phase system were studied, and its anti glycosylation effect was analyzed. On the basis of determining the extraction system as tert butyl alcohol/maltose aqueous two-phase system, the amount of Spirulina platensis powder, ultrasonic time and ultrasonic power were taken as independent variables, and the yield of β-carotene was taken as dependent variable. The results showed that, in the system of 3.4 g tert-butanol/2.4 g maltose, the amount of Spirulina powder was 0.05 g, fill up with water to 10g, under the ultrasonic power of 90 W and ultrasonic time of 5 min, the extraction yield of β-carotene from Spirulina was 3.19 mg/g. When β-carotene concentration was at ranges of 50 to 450 μg/mL, the highest inhibition rate of AGEs was 47.28%, which could provide a reference for the development of novel AGEs inhibitors.
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Key words:
- β-carotene /
- Spirulina platensis /
- ultrasonic extraction /
- t-butanol/maltose system /
- anti-glycosylation
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图 2 叔丁醇加入量对螺旋藻中β-胡萝卜素萃取效果的影响
Figure 2. Effect of tert butyl alcohol on the extraction of β-carotene from Spirulina platensis
图 3 麦芽糖加入量对螺旋藻中β-胡萝卜素萃取效果的影响
Figure 3. Effect of maltose addition on extraction of β-carotene from Spirulina platensis
图 4 螺旋藻粉末加入量对螺旋藻中β-胡萝卜素萃取效果的影响
Figure 4. Effect of Spirulina powder addition on the extraction of β-carotene from Spirulina platensis
图 5 超声功率对螺旋藻中
β-胡萝卜素萃取效果的影响 Figure 5. Effect of ultrasonic power on extraction of β-carotene from Spirulina platensis
图 6 超声时间对螺旋藻中β-胡萝卜素萃取效果的影响
Figure 6. Effect of ultrasonic time on extraction of β-carotene from Spirulina platensis
图 7 β-胡萝卜素及阳性对照组AG在模拟反应体系下的糖基化抑制率
Figure 7. Glycation inhibition rates of positive control group (AG) and β-carotene in model system
表 1 因素水平设计
Table 1. Factors and levels
水平 A螺旋藻粉末加入量（g） B超声功率（W） C超声时间（min） 1 0.025 80 5 2 0.050 90 8 3 0.075 100 11 
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表 2 正交试验设计及结果
Table 2. Orthogonal test design and result analysis
实验号 因素 β-胡萝卜素
得率（mg/g）A（螺旋藻
粉末加入量）B（超声
功率）C（超声
时间）D
空白1 1 1 1 1 2.89 2 1 2 2 2 2.96 3 1 3 3 3 2.81 4 2 1 2 3 2.89 5 2 2 3 1 2.84 6 2 3 1 2 2.99 7 3 1 3 2 2.68 8 3 2 1 3 2.89 9 3 3 2 1 2.85 k1 2.89 2.82 2.92 2.86 k2 2.91 2.90 2.90 2.88 k3 2.81 2.88 2.78 2.86 R 0.10 0.08 0.14 0.02
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表 3 SPSS方差分析数据表
Table 3. SPSS ANOVA data table
方差来源 III 型平方和 df 均方 F Sig. 校正模型 0.063a 6 0.011 19.671 0.049 截距 73.811 1 73.811 137935.855 0.000 A-螺旋藻粉末加入量 0.015 2 0.008 14.343 0.065 B-超声功率 0.013 2 0.007 12.462 0.074 C-超声时间 0.034 2 0.017 32.208 0.030 D-误差 0.001 2 0.001 总计 73.875 9 校正的总计 0.064 8
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