荧光定量PCR技术在食品快速检测中的应用

丁博群 刘珊娜

丁博群,刘珊娜. 荧光定量PCR技术在食品快速检测中的应用[J]. 食品工业科技,2021,42(7):366−373. doi:  10.13386/j.issn1002-0306.2020050185
引用本文: 丁博群,刘珊娜. 荧光定量PCR技术在食品快速检测中的应用[J]. 食品工业科技,2021,42(7):366−373. doi:  10.13386/j.issn1002-0306.2020050185
DING Boqun, LIU Shanna. Application of Real-time Quantitative PCR Technology in Food Rapid Detection[J]. Science and Technology of Food Industry, 2021, 42(7): 366−373. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2020050185
Citation: DING Boqun, LIU Shanna. Application of Real-time Quantitative PCR Technology in Food Rapid Detection[J]. Science and Technology of Food Industry, 2021, 42(7): 366−373. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2020050185

荧光定量PCR技术在食品快速检测中的应用

doi: 10.13386/j.issn1002-0306.2020050185
基金项目: 天津市教委科研计划项目(2019KJ042)
详细信息
    作者简介:

    丁博群(1996−),女,硕士研究生,研究方向:食品加工与安全,E-mail:dingboqun@126.com

    通讯作者:

    刘珊娜(1984−),女,博士,副教授,研究方向:食品生物技术,E-mail:lsn_nxy@aliyun.com

  • 中图分类号: TS207.3

Application of Real-time Quantitative PCR Technology in Food Rapid Detection

  • 摘要: 荧光定量聚合酶链式反应(real-time quantitative polymerase chain reaction,qPCR)是一种将聚合酶链式反应(polymerase chain reaction,PCR)扩增与实时检测相结合的技术,也是近年来食品快速检测技术的研究热点之一。与传统PCR技术相比,它具有耗时短、操作简单、灵敏度高、特异性强等优势。本文简要概述了荧光定量PCR技术的基本原理、发展历程、分类及特点,综述了荧光定量PCR技术在食源性致病菌、掺杂掺假、转基因食品、过敏原、食源性寄生虫、可食用昆虫等食品检测中的应用,并对荧光定量PCR技术及其在食品快速检测中应用的前景进行了展望。
  • 表  1  荧光定量PCR在食源性致病菌检测中的应用情况

    Table  1.   Application of real-time qPCR in the detection of food-borne pathogens

    致病菌种类被检食品荧光定量PCR技术参考
    文献
    单增李斯特菌肉制品TaqMan探针[21]
    鲜切甜瓜[22]
    鲜切蔬菜、牛奶、奶酪[23]
    冷冻蔬菜[24]
    生禽肉、动物性海产品、鲜鸡蛋、熟肉制品、
    中式凉拌菜、米面制品、坚果类、蔬菜
    荧光探针[25]
    大肠杆菌猪肉、牛奶TaqMan探针[26]
    牡蛎[27]
    生菜、卷心菜荧光探针[28]
    志贺氏菌牛奶SYBR Green染料[29]
    乳品TaqMan探针[30]
    冷冻蔬菜[24]
    鲜猪肉[31]
    生禽肉、动物性海产品、鲜鸡蛋、熟肉制品、
    中式凉拌菜、米面制品、坚果类、蔬菜
    荧光探针[25]
    沙门氏菌乳品TaqMan探针[30]
    奶粉[32]
    肉制品[33]
    冷冻蔬菜[24]
    牛乳荧光染料[34]
    冷冻肉制品荧光探针[35]
    生禽肉、动物性海产品、鲜鸡蛋、熟肉制品、
    中式凉拌菜、米面制品、坚果类、蔬菜
    [25]
    金黄色葡萄球菌肉类、乳制品SYBR Green II染料[36]
    奶粉TaqMan探针[32]
    冷冻蔬菜[24]
    生禽肉、动物性海产品、鲜鸡蛋、熟肉制品、
    中式凉拌菜、米面制品、坚果类、蔬菜
    荧光探针[25]
    蜡样芽胞杆菌肉制品TaqMan探针[37]
    鲜牛奶荧光探针[38]
    生禽肉、动物性海产品、鲜鸡蛋、熟肉制品、
    中式凉拌菜、米面制品、坚果类、蔬菜
    [25]
    副溶血性弧菌水产品SYBR Green I染料[39]
    生禽肉、动物性海产品、鲜鸡蛋、熟肉制品、
    中式凉拌菜、米面制品、坚果类、蔬菜
    荧光探针[25]
    酿脓链球菌新鲜猪肉TaqMan探针[40]
    克罗诺杆菌奶粉TaqMan探针[32]
    下载: 导出CSV

    表  2  标准培养方法、常规PCR与荧光定量PCR比较

    Table  2.   Comparison of standard culture method, conventional PCR and real-time qPCR

    检测方法效率灵敏度成本仪器及操作参考文献
    标准培养方法费时(约4~7 d),步骤繁多,检测速度慢无需特殊仪器,操作简单易行[4748]
    常规PCR检测速度较快较高较高操作复杂,易污染,需进行电泳分析[23]
    荧光定量PCR耗时较少(可48 h内完成),操作步骤简便,检测速度快需要荧光定量PCR仪,需培训专业技术人员[49]
    下载: 导出CSV

    表  3  荧光定量PCR在过敏原检测中的应用

    Table  3.   Application of real-time qPCR in the detection of allergens

    食品类型过敏原检测限检测方法目的基因参考文献
    商业食品核桃2.5 pgSYBR GreenJug r 1, 3, 4[65]
    坚果糊榛子2 mg/kgTaqManhsp 1[66]
    板栗粉与小麦粉混合物板栗100 mg/kgTaqManCas s 9[67]
    商业食品花生、榛子、开心果、杏仁、
    腰果、澳洲坚果、核桃、山核桃
    0.1 mg/kgTaqManITS[68]
    商业食品小麦、荞麦、花生10 mg/kgTaqMan18S r RNA[69]
    实验室样品大豆、芹菜0.01%TaqManatp A, mtd[70]
    饼干,婴儿食品,酸奶猕猴桃25 mg/kgSYBR GreenAct c2[71]
    20 mg/kgPru p2.01B
    苹果50 mg/kgMal d1
    功能性食品油菜籽蜂花粉1 pg/μL~1 ng/μLTaqMan-QSYPEP[72]
    茶花蜂花粉mat K
    莲花蜂花粉ITS
    松花粉mat K
    玫瑰蜂花粉ITS
    杨柳蜂花粉ITS
    虞美人蜂花粉ITS
    猕猴桃蜂花粉ITS
    调味酱0.01 pgTaqMan,Eva Green16S rRNA[73]
    调味酱0.1 pgTaqMan16S rRNA[74]
    猪肉饺子、通心粉沙拉、蛤蜊汁螃蟹0.1~105 mg/kg12S rRNA[75]
    食用油、蛤蜊汤、炸玉米饼混合物大西洋鳕鱼、太平洋鳕鱼、白眼狭鳕鱼1~10 mg/kgCOI[76]
    牛奶α-乳清蛋白0.05 ngTaqMan-MGBα-LA[64]
    火腿、香肠牛奶蛋白6 fgTaqMan12S rRNA[77]
    下载: 导出CSV
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出版历程
  • 收稿日期:  2020-05-18
  • 网络出版日期:  2021-01-28
  • 刊出日期:  2021-04-01

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