Abstract: A rapid method for the determination of 19 antihistamines and metabolites residues in livestock and poultry products by UPLC-Q-TOF/MS was developed. Samples,were extracted with acetonitrile-ethyl acetate solution extract by enhanced matrix removal(EMR)combined with oxidative modification of multi-walled carbon nanotubes(MWCNTs)purification,with 0.1% formic acid formic acid water-0.1% acetonitrile as mobile phase in gradient elution,in EclipsePlus-C₁₈(50 mm×2.1 mm,1.8 μm)on the chromatographic column separation,column temperature 40 ℃,the flow rate of 0.3 mL/min,sample quantity was 5 μL,Q-TOF/MS electrospray cations pattern analysis. The first-order mass spectrometry data were collected in the full-scan mode to quantify the peak area of the excimer ion peak of the measured object,and to determine the quality of the characteristic information such as retention time,accurate mass number and isotope abundance ratio. In the Target MS/MS mode,the secondary mass spectrometry data were targeted and further confirmed by the accurate mass number of the characteristic fragment ions. Statistics indicated that this method whose RSD was 3.2%~8.2%,had a good linear with R²>0.996 in their ranges of linearity,what’s more,the LODs of which were 0.5~2.0 μg/kg and the LOQs were 1.5~6.0 μg/kg along with a hige recovery rate ranged 78.2%~105.3% from different sample matrixes(beef,chicken,pork liver)added 3 levels of concentration.. This method is simple,rapid,sensitive,selective and accurate,and is suitable for rapid screening and quantitative determination of 19 antihistamines and metabolites in livestock and poultry products.