Abstract: This study aims to understand the characteristics of cyclic adenosine monophosphate(cAMP)and cyclic guanosine monophosphate(cGMP)contents in different jujube germplasm resources and select the higher content germplasm,thus to provide reference for further development and utilization resources. The contents of cAMP and cGMP in crisp mature fruits of 204 jujube germplasm resources were determined by using ultra high performance liquid chromatography(UPLC),and variation,correlation and cluster analysis were performed. The results showed that cAMP content was higher than cGMP for the same resource. The mean contents of cAMP and cGMP in crisp mature fruits of 204 resources were 58.01 μg·g^-1·FW and 22.82 μg·g^-1·FW,the variation coefficient were 53.77% and 58.81%,respectively. Both of them were normally distributed. The cAMP content significanty positive correlation with the cGMP content. Cluster analysis in 4.5 genetic distance can divide 204 resources into 3 groups,which were defined as high content group,middle content group and low content group,respectively. 28 jujube resources with high contents of cAMP and cGMP,including ‘Xupuxiangzao’,‘Linzedazao’,‘linyibenzao’,‘Gendedazao’,‘Yanchuandieyazao’ and so on,were screened out. In this study,we established the classification index for jujube cAMP and cGMP contents and selected the specific germplasms with higher contents,which can provide a theoretical basis for the selection of foundation materials for the processing of cAMP and cGMP functional food.