摘要:
为了研究毛蕊花苷的稳定性,本文采用高效液相色谱法研究毛蕊花苷在不同温度(37、50、60、80、100 ℃)、不同pH(2、6、7、8)、光照/避光条件下的降解动力学。结果表明,毛蕊花苷在不同温度、pH和光照/避光条件下的降解反应均属于一级反应。对毛蕊花苷半衰期的计算结果表明,高温(60 ℃以上)、近中性环境(pH6~8)、光照均不利于毛蕊花苷的保存;毛蕊花苷在中低温(37、50 ℃)、低pH(2)及避光条件下更稳定,半衰期明显延长。本文分析了影响毛蕊花苷稳定性的主要因素,可为毛蕊花苷提取、纯化和活性分析等相关研究提供重要的参考。
Abstract:
In order to study the stability(the thermal,light and pH stability)of verbascoside,this paper discussed the effects of temperature(37,50,60,80,100 ℃),pH(2,6,7,8)and light on the degradation kinetics of verbascoside by HPLC. The results showed that the degradation reaction of verbascoside in different temperature,pH and light/dark conditions belonged to the first order reaction. The results showed that high temperature(above 60 ℃),near neutral environment(pH6~8)and light were not conducive to the preservation of verbascoside. The half-life of verbascoside was more stable at medium low temperature(37,50 ℃),low pH(2)and dark conditions,and the half-life was significantly prolonged. In this paper,the main factors affecting the stability of verbascoside were analyzed,which could provide an important reference for the extraction,purification and activity analysis of verbascoside.
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