Abstract: Objective:The purpose of this study was to establish a simple,rapid and highly sensitive immunochromatographic method for the determination of quinolones(ciprofloxacin,enrofloxacin,norfloxacin,levofloxacin,and so on) in yak meat. Methods:Ciprofloxacin mouse monoclonal antibody G10785 was coupled with carboxylated europium microspheres,and ciprofloxacin antigen and goat anti-mouse antibody were coated on cellulose nitrate membrane(NC) as detection line(T) and control line(C),respectively. Quantitative standard curves were established according to the value of T/C and quinolone concentration in samples. The sensitivity,accuracy,and precision of the method were evaluated,and the yak meat samples were analyzed by using both the developed method and LC-MS/MS. Results:The limit of quantitation of the established method was lower than 0.96 μg/kg,the in-batch recovery rate ranged from 82.94% to 111.27%,and the coefficient of variation ranged from 4.13% to 9.90%,the inter-assay recovery rate ranged from 88.86%~107.05%,and the coefficient of variation ranged from 3.86%~9.39%. The cross-reactivity rates of the established method with ciprofloxacin,enrofloxacin,norfloxacin,levofloxacin,and danofloxacin were 100%,77.36%,107.66%,93.55%,and 50.92%,respectively. Conclusion:The time-resolved fluorescence immunochromatographic method established in this study had high accuracy,precision,sensitivity and stable performance,and had a good application prospect.