Abstract: In this study,solution-stirring method was used to prepare sanguinarine and HP-β-CD inclusion complex. Taking the inclusion ratio of HP-β-CD and sanguinarine,the inclusion temperature,and the inclusion time as the influencing factors,based on the single factor test,the Box-Behnken center combination method was used to perform 3 factors and 3 levels. The experimental design was based on the response values of sanguinarine and HP-β-CD inclusion rate,and the response surface method was used to further optimize the inclusion conditions. The results showed that the best conditions were:inclusion ratio 10:1,inclusion temperature 60℃,inclusion time 190 min,The inclusion rate of sanguinarine and HP-β-CD was 73.55%,which was close to the predicted value(72.43%) (relative error 1.5%). The method was stable and repeatable,and was an efficient method for preparing sanguinarine HP-β-CD inclusion compound.