2种新疆沙棘中黄酮、多酚及其抗氧化活性分析

王苗苗; 刘宗浩; 张永; 严欢; 田合; 毛琼玲; 李慕春

doi:10.13386/j.issn1002-0306.2020.18.008

2种新疆沙棘中黄酮、多酚及其抗氧化活性分析

Analysis of the Flavonoid,Polyphenol and Its Antioxidant Activity of 2 Kinds of Sea Buckthorn from Xinjiang

摘要

摘要: 以新疆2种沙棘为研究对象,使用分光光度法、超高效液相色谱法对其黄酮(槲皮素、山奈酚、异鼠李素)、多酚含量及抗氧化活性(DPPH自由基、铁离子自由基、还原力、总抗氧化能力、铁离子螯合能力)进行比较分析。结果表明,沙棘A黄酮、多酚含量分别为(7.02±0.19)、(5.48±0.42)mg·g^-1,沙棘B黄酮、多酚含量分别为(9.56±0.53)、(24.33±1.44)mg·g^-1,沙棘B的黄酮、多酚含量均高于沙棘A。沙棘A、B的DPPH清除率IC₅₀浓度值分别为(128.953±0.098)和(7.521±0.027)μg·mL^-1;当沙棘浓度为1 mg·mL^-1时,沙棘A的FRAP值、T-AOC值、还原力测定值和铁离子螯合测定值分别为(0.5611±0.0042)mmol·L^-1、(6.0457±0.3402)U·mL^-1、(0.2071±0.0113)和(0.3003±0.0164),沙棘B的FRAP值、T-AOC值、还原力测定值和铁离子螯合测定值分别为浓度为(0.4853±0.0067)mmol·L^-1、(4.5318±0.1553)U·mL^-1、(0.2399±0.0104)和(0.8851±0.0077);由此可见,二者抗氧化活性差别不大,沙棘B略高于沙棘A;沙棘A的槲皮素、山奈酚、异鼠李素含量分别为(0.6708±0.0410)、(0.0788±0.0292)和(1.5814±0.0372)mg·g^-1,沙棘B的槲皮素、山奈酚、异鼠李素含量分别为(0.9438±0.0403)、(0.1194±0.0153)和(1.0727±0.0406)mg·g^-1;沙棘B的槲皮素、山奈酚含量均高于沙棘A,而沙棘A的异鼠李素含量明显高于沙棘B。综上,沙棘B具有较高的黄酮、多酚含量及略优的抗氧化活性。

Abstract: This article aimed to compare the flavone content(quercetin,kaempferol and isorhamnetin),phenol content and the in vitro antioxidant capacity(DPPH free radical scavenging ability,ferric ion reducing antioxidant power assay,reducing power,total antioxidant capacity and Fe²⁺ chelating activity) of two kinds of sea buckthorn by spectrophotometry analysis and UPLC method. The contents of flavone and phenol in sea buckthorn A were(7.02±0.19),(5.48±0.42) mg·g^-1,respectively. The contents of flavone and phenol in sea buckthorn B were(9.56±0.53),(24.33±1.44) mg·g^-1,respectively. The results showed that the contents of flavone and phenol in sea buckthorn B were markedly higher than those of sea buckthorn A. The IC₅₀ of DPPH free radical scavenging rate for sea buckthorn A and sea buckthorn B were(128.953±0.098),(7.521±0.027) μg·mL^-1,respectively. When the concentration was 1 mg·mL^-1,the FRAP,T-AOC,reducing power and Fe²⁺ chelating activity of sea buckthorn A were(0.5611±0.0042) mmol·L^-1,(6.0457±0.3402) U·mL^-1,(0.2071±0.0113) and(0.3003±0.0164),respectively. In addition,the FRAP,T-AOC,reducing power and Fe²⁺ chelating activity of sea buckthorn B were(0.4853±0.0067) mmol·L^-1,(4.5318±0.1553) U·mL^-1,(0.2399±0.0104) and(0.8851±0.0077),respectively. In the antioxidant capacity,there was no significant difference between the two kinds of sea buckthorn,and the antioxidant capacity of sea buckthorn B was slightly better than that of the sea buckthorn A. The contents of quercetin,kaempferoll and isorhamnetin in sea buckthorn A were(0.9438±0.0403),(0.1194±0.0153),(1.0727±0.0406) mg·g^-1. The contents of quercetin,kaempferoll and isorhamnetin in sea buckthorn B were(0.9438±0.0403),(0.1194±0.0153) and(1.0727±0.0406) mg·g^-1. The contents of quercetin and kaempferoll in sea buckthorn B were higher than those of sea buckthorn A,but the content of isorhamnetin in sea buckthorn B was markedly less than that of the sea buckthorn A. In summary,the sea buckthorn B had better antioxidant activity,and higher amounts of total flavone and total phenol.

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