Abstract: This article mainly explored the effects of the germination process of Qingke on its β-glucan content,molecular weight,anti-oxidation and anti-inflammatory effect. The results showed that during the germination process,the content of β-glucan increased at first and then decreased,and the molecular weight decreased from 275.74 kDa to 114.63 kDa;Compared with the non-germinated Qingke beta-glucan,the FRAP value,DPPH and ABTS inhibition rate of germinated Qingke beta-glucan had increased by 35.63%,248.64% and 80.57% respectively. In addition,it was found that germinated Qingke β-glucan could improve the cell survival rate,reduce the secretion levels of inflammatory factors TNF-α,IL-6 and pro-inflammatory factors PGE₂,NO,decrease the production of MDA,increase the content of SOD and GSH to improve the level of oxidative stress during cell inflammation significantly by establishing an LPS-induced Caco-2 cell inflammation model. In summary,germination treatment could significantly enhance the function of Qingke β-glucan to relieve intestinal inflammation damage.