马氏珍珠贝中核苷类成分的分离富集工艺研究

张焱; 杨书婷; 韦小翠; 颜媛媛; 朱宇超; 嵇晶; 王琪; 张晓云; 何瑞欣; 徐雅蝶; 程建明

doi:10.13386/j.issn1002-0306.2020.17.029

马氏珍珠贝中核苷类成分的分离富集工艺研究

Study on the Separation and Enrichment of Nucleoside Components in Pinctada martensii

摘要

摘要: 目的:对马氏珍珠贝中核苷类成分进行分离富集工艺研究,以期得到纯度较高的天然核苷。方法:以马氏珍珠贝水提醇沉液中的核苷类成分为指标,采用HPLC测定含量,对大孔树脂型号、上样工艺、洗脱工艺进行考察,采用UV对纯化部位的核苷类成分进行测定。结果:根据HPLC-UV所得结果,选定大孔树脂型号为SP207、上样浓度为2.19 mg/mL、上样pH为4.6、上样流速为2 BV/h,核苷最佳上样体积为25 mL,洗脱时水洗量为2 BV,洗脱溶剂选择15%乙醇,洗脱流速选择3 BV/h,洗脱体积选择为3 BV,径高比选择1:7。结论:经SP207纯化后,总核苷纯度为58.19%,表明该方法适用于分离富集马氏珍珠贝中的核苷类成分。

Abstract: Objective:In order to obtain natural nucleosides with higher purity,the separation and enrichment process of nucleosides in Pinctada martensii was studied. Methods:The nucleosides from the liquid decocted with water and deposited with alcohol of Pinctada martensii were used as indicators to determine the type of macroporous resin by HPLC-UV,the loading process and the elution process.The nucleoside at the purified site was determined by UV. According to the results obtained by HPLC-UV,the selected macroporous resin model was SP207,the loading concentration was 2.19 mg/mL,the loading pH was 4.6,the loading flow rate was 2 BV/h,and the optimal loading volume of nucleoside was 25 mL. The elution time was 2 BV,the elution solvent was 15% ethanol,the elution flow rate was 3 BV/h,the elution volume was 3 BV,and the aspect ratio was 1:7. Conclusion:After SP207 purification,the purity of total nucleosides was 58.19%,which indicated that this method was suitable for the separation and enrichment of nucleosides in Pinctada martensii.

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