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中国精品科技期刊2020
张天花, 曾慧慧, 樊自强, 杨莉, 段永霞, 叶帆, 杨柳, 余邦良. 超声法提取海南眼树莲总三萜的工艺优化及其抗氧化性[J]. 食品工业科技, 2020, 41(15): 167-172. DOI: 10.13386/j.issn1002-0306.2020.15.026
引用本文: 张天花, 曾慧慧, 樊自强, 杨莉, 段永霞, 叶帆, 杨柳, 余邦良. 超声法提取海南眼树莲总三萜的工艺优化及其抗氧化性[J]. 食品工业科技, 2020, 41(15): 167-172. DOI: 10.13386/j.issn1002-0306.2020.15.026
ZHANG Tian-hua, ZENG Hui-hui, FAN Zi-qiang, YANG Li, DUAN Yong-xia, YE Fan, YANG Liu, YU Bang-liang. Optimization of Ultrasonic Extraction of Total Triterpenoids from Hainan Dischidia chinensis and Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2020, 41(15): 167-172. DOI: 10.13386/j.issn1002-0306.2020.15.026
Citation: ZHANG Tian-hua, ZENG Hui-hui, FAN Zi-qiang, YANG Li, DUAN Yong-xia, YE Fan, YANG Liu, YU Bang-liang. Optimization of Ultrasonic Extraction of Total Triterpenoids from Hainan Dischidia chinensis and Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2020, 41(15): 167-172. DOI: 10.13386/j.issn1002-0306.2020.15.026

超声法提取海南眼树莲总三萜的工艺优化及其抗氧化性

Optimization of Ultrasonic Extraction of Total Triterpenoids from Hainan Dischidia chinensis and Its Antioxidant Activity

  • 摘要: 目的:优化眼树莲中总三萜的提取工艺,并研究其抗氧化能力。方法:采用超声方法提取眼树莲中三萜,并利用分光光度法测定其含量。首先利用单因素实验确定最初实验条件,在单因素基础上,使用L3(34)正交实验优化工艺。用DPPH·法测定优化后提取的总三萜抗氧化性。结果:4个因素对总三萜提取量影响大小为料液比 > 温度 > 超声功率 > 超声时间,最佳提取工艺为采用提取溶剂为乙酸乙酯,超声时间为30 min,料液比为1∶20 (g/mL),超声功率为200 W,温度为55 ℃,眼树莲中总三萜平均提取量为20.82 mg/g。该工艺提取获得的眼树莲总三萜浓度为2.00 mg/mL时,对DPPH·的清除率为26.86%,其抗氧活性低于VC。结论:与传统溶剂提取、索氏提取相比,优化后的超声提取工艺提取眼树莲总三萜的提取量明显较高,平均提取量为20.82 mg/g,获得的眼树莲总三萜具有一定的抗氧化活性。

     

    Abstract: Objective:To optimize the extraction process of total triterpenoids from Dischidia chinensis and to study the antioxidant capacity. Methode:Triterpenoids were extracted from Dischidia chinensis by ultrasound and their contens were determinated with spectrophotometry. Firstly, the initial test conditions were determined by single factor test. L3 (34) orthogonal test was used to optimize the process based on single factor experiment. The antioxidant activity of total triterpenoids extracted after optimization by DPPH·.Results:The effects ability of four factors on the yield of total triterpenoids were as follows:Material-liquid ratio > temperature > ultrasound power > ultrasound time. The optimum extraction process was that the solvent was ethyl acetat, ultrasound time was 30 min, material-liquid ratio was 1:20 (g/mL), ultrasound power was 200 W, temperature was 55℃. And the average yield of total triterpenoids from Dischidia chinensis was 20.82 mg/g. When the concentration of total triterpenoids extracted by this process was 2.00 mg/mL, the DPPH removal rate was 26.86%, but its antioxidant activity was lower than that of VC. Conclution:Compared with traditional solvent extraction and Soxhlet extraction, the yield of total triterpenoids extracted by the optimized ultrasonic extraction process was obviously higher, and the average yield was 20.82 mg/g. The obtained total triterpenoids from Dischidia chinensis had certain antioxidant activity.

     

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