Abstract: Objective:To investigate the protective effect of LBP on liver injury induced by acrylamide (AA) in rats. Methods:70 healthy adult rats were randomly divided into 7 groups:AA infected group (AA, gavaged AA aqueous solution 20 mg/kg bw), positive control group (NAC+AA, gavaged AA aqueous solution 20 mg/kg bw and N-acetyl cysteine aqueous solution 200 mg/kg bw), toxic suppression group (LBP+AA, divided into low, medium and high dose groups, gavaged AA aqueous solution 20 mg/kg bw and LBP 50 mg/kg bw, 100 mg/kg bw, 150 mg/kg bw), polysaccharide high dose group (LBPH, gavaged LBP 200 mg/kg bw) and normal group (CT, gave the same volume of saline). The drugs was given continuously for 4 weeks, the rats were weighed weekly, after administration, various related indexes were measured and liver tissue structure was observed. Results:The weight loss of rats in the AA-infected group was significant, the SOD, CAT and GSH-Px contents in the liver tissue of the rats were significantly decreased, and the contents of MDA, AST and ALT were significantly increased compared with the CT group (P<0.05). Severe pathological changes were observed in liver tissues in the AA-infected group, with disordered arrangement of hepatic lobules and blurred boundary between hepatocytes. The weight of rats in the LBP groups and the NAC group were significantly improved, the activity of SOD, CAT, GSH-Px in cells were significantly increased, and the contents of MDA, AST and ALT in liver tissues were significantly decreased compared with the AA infected group (P<0.05). The pathological changes of liver tissues were improved, the liver cells were arranged normally, the cell boundaries were clear and the cell structure was complete in NAC+AA group and LBP+AA groups. Conclusion:Lycium barbarum polysaccharide can improve the liver injury of AA infected rats and improve the level of anti-oxidation in the liver.