Abstract: The isolation, purification and structure dentification of polysaccharide from Fagopyrum esculentum Moench bee pollen was studied in this paper. The polysaccharide was extracted from Fagopyrum esculentum Moench bee pollen with extraction in water and alcohol precipitation. The crude polysaccharide was purified using papain treatment combined with Sevag method and DEAE-52 cellulose column chromatography, and three polysaccharide fractions (WFPP-N、WFPP-1、WFPP-2) were obtained.The chemical structures of main components were characterized by ultraviolet spectroscopy (UV), gas chromatography-mass spectrometry (GC-MS), high performance liquid chromatography (HPLC), and infrared spectroscopy (IR).The results showed that the three fractions did not contain proteins, nucleic acid, and so on. All polysaccharide fractions were composed of arabinose, glucose, galactose, rhamnose, xylose, mannose.The monosaccharide components in different polysaccharide fractions had different molar ratio. The molecular weights of the WFPP-1, WFPP-2 were 1.96×10⁴ and 2.24×10⁴ Da, respectively. And the molecular weight of WFPP-2 contained two components were 2.40×10⁴, 4.38×10³ Da. The infrared spectrum of the polysaccharide showed that all fractions had polysaccharide characteristic absorption peak. In this paper, three polysaccharide components isolated from the WFPP were analyzed and identified, which provided a reference for studying the functional activity of polysaccharide from Fagopyrum esculentum Moench bee pollen in the future.