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中国精品科技期刊2020
庞博文, 王勤志, 王俊涛, 夏宁, 滕建文, 韦保耀, 黄丽. 基于DNA检测的 肉制品鉴伪技术研究进展[J]. 食品工业科技, 2020, 41(8): 333-340,346. DOI: 10.13386/j.issn1002-0306.2020.08.054
引用本文: 庞博文, 王勤志, 王俊涛, 夏宁, 滕建文, 韦保耀, 黄丽. 基于DNA检测的 肉制品鉴伪技术研究进展[J]. 食品工业科技, 2020, 41(8): 333-340,346. DOI: 10.13386/j.issn1002-0306.2020.08.054
PANG Bo-wen, WANG Qin-zhi, WANG Jun-tao, XIA Ning, TENG Jian-wen, WEI Bao-yao, HUANG Li. Advances in DNA-Based Identification Techniques for Adulteration of Meat Products[J]. Science and Technology of Food Industry, 2020, 41(8): 333-340,346. DOI: 10.13386/j.issn1002-0306.2020.08.054
Citation: PANG Bo-wen, WANG Qin-zhi, WANG Jun-tao, XIA Ning, TENG Jian-wen, WEI Bao-yao, HUANG Li. Advances in DNA-Based Identification Techniques for Adulteration of Meat Products[J]. Science and Technology of Food Industry, 2020, 41(8): 333-340,346. DOI: 10.13386/j.issn1002-0306.2020.08.054

基于DNA检测的 肉制品鉴伪技术研究进展

Advances in DNA-Based Identification Techniques for Adulteration of Meat Products

  • 摘要: 在利益的驱动下,肉制品掺假行为屡禁不止,因此肉制品鉴伪技术逐步发展。本文综述了近五年来常见的基于DNA检测的肉制品鉴伪技术,包括经典的PCR、qPCR、DNA条码技术,新兴技术如下一代测序技术、极灵敏的HRM、用于基因诊断的LAMP,经典DNA标记如微卫星技术、RAPD、AFLP、RFLP、序列特征扩增区域、单核苷酸多态性等,为肉制品掺假鉴伪等相关研究提供借鉴。

     

    Abstract: Driven by interests,the adulteration of meat products has been repeatedly prohibited. Therefore,the identification technology for adulteration of meat products have gradually been developed. In this paper,the common DNA-based adulteration identification techniques for meat products in recent five years are reviewed,including classical PCR,qPCR and DNA barcode techniques. Emerging technologies include the following generation of sequencing technology,extremely sensitive HRM,LAMP for gene diagnosis,microsatellite technique,RAPD,AFLP,RFLP,sequence characteristic amplification region,single nucleotide polymorphism and others. It provides reference for the study of adulteration and forgery of meat products.

     

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