Abstract: Kjeldahl method,Soxhlet extraction method and atomic absorption spectrometry were used to determine essential nutrients and active ingredients,amino acids and minerals in the leaves of Paeonia suffruticosa Andr.from three different regions in Heze. The results showed that the protein,essential amino acid and medicinal amino acid contents of leaves of Paeonia suffruticosa Andr.from Yuncheng were the highest,which were 21.9%,8.01 g/100 g and 12.61 g/100 g,respectively. The essential amino acid index(EAAI)of leaves of Paeonia suffruticosa Andr.from Yuncheng was 0.79,which was the available protein source. Leaves of Paeonia suffruticosa Andr.from Chengwu contained the highest contents of total fatty acid,which was 0.4400%. Leaves of Paeonia suffruticosa Andr. from Caoxian had the highest vitamin C and calcium contents,which were 54.8 mg/100 g and 1.51 g/100 g,respectively. As a consequence,leaves of Paeonia suffruticosa Andr.were rich in essential nutrients and reasonable in composition. The nutritional and active components of leaves of Paeonia suffruticosa Andr.from three different regions were distinct,thus the region of materials should be selected according to the demand of development products.