Abstract: To evaluate the antioxidant effect of polysaccharide from Hippophae rhamnoides(PBP)on subacute aging mice induced by D-galactose,the mice were divided into blank group,aging model group,positive group(V_E170 mg/kg),low(100 mg/kg),middle(200 mg/kg)and high(400 mg/kg)dose group of PBP,and fed continuous gavage for 30 d. The viscera indexes,activities of SOD,GSH-Px and MDA content in liver,brain and serum of each group were determined. The changes of liver and brain cells and structures were observed by HE staining.The results showed that,compared with the model group,the body mass of mice biggest increase were high dose PBP group and V_E group,and the brain,heart and spleen index of mice in high,middle PBP and V_E group increased significantly compared with the model group(P<0.05,P<0.01).GSH-Px in liver,brain and serum of high,medium PBP group were higher than those in model group(P<0.01),SOD activity in liver,brain and serum of high PBP group were higher than those in model group(P<0.01). MDA content in liver,brain and serum of high dose and V_E group were lower than those in model group(P<0.01).HE staining results of tissue sections showed that PBP had protective effect on liver,brain tissue cells and structure damage induced by aging in mice. The protective effect of PBP on D-galactose induced subacute aging mice may be related to its antioxidant effect.