Abstract: Prepared the flavones reference extract from Ziziphus jujuba seed was substituted for single reference substance to determine the ingredients(spinosin,6-feruloylspinosin)of Zaoren Anshen granules to explore the feasibility for quality control of traditional Chinese medicine. The results showed that there was a good linearity between the concentration of spinosin,6-feruloylspinolin in the flavones reference extract and the peak area value in ranges of 0.0087~0.4366(r=0.9996),0.0039~0.1952 mg·mL^-1(r=0.9996),respectively. The average recoveries of two components were 100.26%,100.90% with RSDs which were 2.45%,1.37%. The results of 12 batches of Zaoren Anshen granules were determined by utilizing the reference extract of Semen Zizipon seed as control,which were basically consistent with those obtained by single reference substance. The results indicated that the reference extract could replace the corresponding of reference substance to determine the traditional Chinese medicine ingredients,and be used for quality control of Chinese herbal medicines and their preparations.