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中国精品科技期刊2020
徐杰, 李新光, 王建中, 王丰俊. 黑果腺肋花楸果汁的酶解制备工艺优化及其功能性质[J]. 食品工业科技, 2020, 41(1): 125-131,137. DOI: 10.13386/j.issn1002-0306.2020.01.021
引用本文: 徐杰, 李新光, 王建中, 王丰俊. 黑果腺肋花楸果汁的酶解制备工艺优化及其功能性质[J]. 食品工业科技, 2020, 41(1): 125-131,137. DOI: 10.13386/j.issn1002-0306.2020.01.021
XU Jie, LI Xin-guang, WANG Jian-zhong, WANG Feng-jun. Optimization of Enzymatic Hydrolysis for Production of Aronia melanocarpa Juice and Its Functional Properties[J]. Science and Technology of Food Industry, 2020, 41(1): 125-131,137. DOI: 10.13386/j.issn1002-0306.2020.01.021
Citation: XU Jie, LI Xin-guang, WANG Jian-zhong, WANG Feng-jun. Optimization of Enzymatic Hydrolysis for Production of Aronia melanocarpa Juice and Its Functional Properties[J]. Science and Technology of Food Industry, 2020, 41(1): 125-131,137. DOI: 10.13386/j.issn1002-0306.2020.01.021

黑果腺肋花楸果汁的酶解制备工艺优化及其功能性质

Optimization of Enzymatic Hydrolysis for Production of Aronia melanocarpa Juice and Its Functional Properties

  • 摘要: 采用单因素实验及响应面优化分析获得了黑果腺肋花楸果汁的最佳酶解制备条件,并对其功能性质进行了研究。结果表明:黑果腺肋花楸酶解制汁的最佳工艺参数为加酶量0.06%,果胶酶与纤维素酶比例为4:5(m:m),酶解温度为45℃,酶解时间为3.5 h,在此条件下,果汁中原花青素含量为(1679.85±6.11)mg/100 g,果实出汁率为78.65%±1.05%。酶解工艺制备的黑果腺肋花楸果汁具有较强的DPPH自由基清除能力,半数抑制浓度为0.232 mg/mL,同时对α-淀粉酶、酪氨酸酶以及黄嘌呤氧化酶均具有较好的抑制作用,其半数抑制浓度分别为2.326、22.587、30.722 mg/mL,表现出质量浓度依赖性。研究结果可为黑果腺类花楸果汁的产业化生产提供理论依据。

     

    Abstract: The optimal enzymatic conditions for the juice of Aronia melancarpa were obtained by single factor experiment and response surface optimization experiment,and the functional properties were also studied. The results showed that the optimum processing parameters for producing Aronia melanocarpa juice by compound enzyme methord were as follows:The composite enzyme adding amount was 0.06%,the ratio of pectinase to cellulase was 4:5 (m:m),the enzymolysis temperature was 45℃,and the enzymolysis time was 3.5 h. Under this condition,the proanthocyanidin content in the juice was(1679.85±6.11) mg/100 g and the juice yield was 78.65%±1.05%. The Aronia melancarpa juice prepared by enzymatic hydrolysis process has strong 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging activity,with median inhibitory concentration(IC50)of 0.232 mg/mL. At the same time,it has high inhibitory effect on α-amylase,tyrosinase and xanthine oxidase,with median inhibitory concentration(IC50)of 2.326,22.587 and 30.722 mg/mL,respectively,showing mass concentration dependence. These results can provide a theoretical basis for the industrial production of Aronia melancarpa juice.

     

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