发光杆菌产几丁质酶的工艺优化

陈立功; 吴家葳; 张冉; 张庆芳; 迟乃玉; 王晓辉

doi:10.13386/j.issn1002-0306.2019120161

发光杆菌产几丁质酶的工艺优化

doi: 10.13386/j.issn1002-0306.2019120161

陈立功^1,2,
吴家葳^1,2,
张冉^1,2,
张庆芳^1,2,
迟乃玉^1,2,
王晓辉^1,2, ,

1. 大连大学生命科学与技术学院, 辽宁大连 116600;
2. 辽宁省海洋微生物工程技术研究中心, 辽宁大连 116600

基金项目:

国家自然科学基金青年科学基金（31500039）；辽宁省博士启动基金指导计划项目（20170520167）；大连市青年科技之星项目（2017RQ155）；大连大学博士专项基金（2017QL020）；辽宁省自然科学基金（20180550728）。

详细信息

作者简介:
陈立功(1995-),男,硕士研究生,研究方向:海洋微生物及其酶,E-mail:ligong0808@163。com。

通讯作者:
王晓辉(1981-),女,博士,副教授,研究方向:生物化工与酶工程,E-mail:wangxiaohui@dlu.edu.cn。

中图分类号: TS201.3
计量
- 文章访问数: 9
- HTML全文浏览量: 1
- PDF下载量: 3
- 被引次数: 0
出版历程
- 收稿日期: 2019-12-16

Optimization of Chitinase Production by the Photobacterium sp.LG-1

1. School of Life Science and Biotechnology, Dalian University, Dalian 116600, China;
2. Liaoning Marine Microbial Engineering Technology Research Center, Dalian 116600, China

摘要

摘要: 为提高海洋发光杆菌Photobacterium sp.LG-1产低温几丁质酶的酶活性，利用响应面法对其发酵产酶条件进行了优化。Plackett-Burman实验结果表明，影响菌株产酶的三个主要因素分别为发酵温度、发酵时间和酵母膏含量。菌株产酶的最适条件为：胶体几丁质浓度12.0 g/L、酵母膏浓度4.5 g/L、转速220 r/min、发酵温度20℃、装液量75 mL/250 mL、接种量1%、初始pH7.0、发酵时间120 h，几丁质酶的最大酶活为5.10 U/mL。实际平均酶活经验证与预测酶活相近，几丁质酶活比优化前提高了11.50%。为下一步低温几丁质酶的降解机制研究及在工业中的应用提供参考。
- 发光杆菌 /
- 几丁质酶 /
- 发酵条件优化 /
- 响应面法
Abstract: To increase chitinase activity,response surface methodology was used to optimize the fermentation conditions of cold-adapted chitinase by Photobacterium sp. LG-1. The result of Plackett-Burman showed that the important factors influencing chitinase production were fermentation temperature,fermentation time and yeast extract. The optimal fermentation conditions were determined as followed: colloidal chitin 12.0 g/L,yeast extract 4.5 g/L,shaking speed 220 r/min,fermentation temperature 20 ℃,liquid volume 75 mL/250 mL,inoculums dose 1%,pH7.0,and fermentation time 120 h,respectively. Under these conditions,the max enzyme activity of chitinase was 5.10 U/mL.The maximum theoretic value was consistent with mean value of verification test and the chitinase production was increased by 11.50% comparing to that before optimization.It provides a reference for the research on the degradation mechanism of low-temperature chitinase and its application in industry.
- Photobacterium /
- chitinase /
- optimization of fermentation conditions /
- response surface methodology

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