发光杆菌产几丁质酶的工艺优化

陈立功 吴家葳 张冉 张庆芳 迟乃玉 王晓辉

陈立功, 吴家葳, 张冉, 张庆芳, 迟乃玉, 王晓辉. 发光杆菌产几丁质酶的工艺优化[J]. 食品工业科技, 2021, 42(1): 110-114,131. doi: 10.13386/j.issn1002-0306.2019120161
引用本文: 陈立功, 吴家葳, 张冉, 张庆芳, 迟乃玉, 王晓辉. 发光杆菌产几丁质酶的工艺优化[J]. 食品工业科技, 2021, 42(1): 110-114,131. doi: 10.13386/j.issn1002-0306.2019120161
CHEN Li-gong, WU Jia-wei, ZHANG Ran, ZHANG Qing-fang, CHI Nai-yu, WANG Xiao-hui. Optimization of Chitinase Production by the Photobacterium sp.LG-1[J]. Science and Technology of Food Industry, 2021, 42(1): 110-114,131. doi: 10.13386/j.issn1002-0306.2019120161
Citation: CHEN Li-gong, WU Jia-wei, ZHANG Ran, ZHANG Qing-fang, CHI Nai-yu, WANG Xiao-hui. Optimization of Chitinase Production by the Photobacterium sp.LG-1[J]. Science and Technology of Food Industry, 2021, 42(1): 110-114,131. doi: 10.13386/j.issn1002-0306.2019120161

发光杆菌产几丁质酶的工艺优化

doi: 10.13386/j.issn1002-0306.2019120161
基金项目: 

国家自然科学基金青年科学基金(31500039);辽宁省博士启动基金指导计划项目(20170520167);大连市青年科技之星项目(2017RQ155);大连大学博士专项基金(2017QL020);辽宁省自然科学基金(20180550728)。

详细信息
    作者简介:

    陈立功(1995-),男,硕士研究生,研究方向:海洋微生物及其酶,E-mail:ligong0808@163。com。

    通讯作者:

    王晓辉(1981-),女,博士,副教授,研究方向:生物化工与酶工程,E-mail:wangxiaohui@dlu.edu.cn。

  • 中图分类号: TS201.3

Optimization of Chitinase Production by the Photobacterium sp.LG-1

  • 摘要: 为提高海洋发光杆菌Photobacterium sp.LG-1产低温几丁质酶的酶活性,利用响应面法对其发酵产酶条件进行了优化。Plackett-Burman实验结果表明,影响菌株产酶的三个主要因素分别为发酵温度、发酵时间和酵母膏含量。菌株产酶的最适条件为:胶体几丁质浓度12.0 g/L、酵母膏浓度4.5 g/L、转速220 r/min、发酵温度20℃、装液量75 mL/250 mL、接种量1%、初始pH7.0、发酵时间120 h,几丁质酶的最大酶活为5.10 U/mL。实际平均酶活经验证与预测酶活相近,几丁质酶活比优化前提高了11.50%。为下一步低温几丁质酶的降解机制研究及在工业中的应用提供参考。
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  • 收稿日期:  2019-12-16

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