Abstract: The aim of this study was to investigate the regulation and mechanism of Dendrobium officinale Kinura et Migo glycoprotein(DOKMG)on skin inflammation.In this paper,macrophage cell line RAW264.7 was targeted and stimulated by DOKMG in different time and concentration,the mRNA expression levels of inflammatory factor IL-1β,IL-6,TNF-α and inflammation-related COX-2 and iNOS were detected by RT-PCR. The expression of inflammatory factors in supernatant of macrophages was detected by ELISA. Western blot was used to analyze the effect of DOKMG on the expression of inflammatory factor protein and NF-κB signaling pathway. The results showed that DOKMG significantly promoted the expression of IL-1β,IL-6,TNF-α genes after 24 h(P<0.001).When the concentration reached 100 μg/mL,the improvement was the most obvious. Compared with the control group,IL-1β increased by 10.09 times,IL-6 increased by 20.45 times,and TNF-α increased by 0.95 times. When the concentration was 100 μg/mL,the secretion of TNF-α、IL-1β increased from 0.52 μg/mL and 118.52 pg/mL to 1.99 μg/mL and 272.81 pg/mL,the expression of inflammatory cytokines and phosphorylation of NF-κB were significantly promoted(P<0.05).These results indicate that DOKM can significantly promote macrophage inflammatory response to promote wound healing,and its mechanism may be related to the activation of NF-κB signaling pathway.