Abstract: In order to compare the amino acid components in different kinds of passion fruit,the amino acid composition and nutritional value of 5 passion fruit juice were analyzed by an automatic amino acid analyzer,and significance analysis and cluster analysis were performed to compare their differences. The results indicated that 17 amino acids were found in all passion fruit samples,‘Guava Taste Golden Fruit’contains the highest amounts of TAA(977.03 mg·100 g^-1),EAA(268.14 mg·100 g^-1)and CEAA(63.26 mg·100 g^-1),which was significantly higher than ‘Shi Sheng Zhu Xi Zi Guo’. In addition,EAA/NEAA(42.13%),EAA/TAA(29.63%)and SRC(72.61)of ‘Shi Sheng Zhu Xi Zi Guo’ were the highest. The score of ratio coefficient(SRC)of amino acids was 64.49~72.61,and the first limiting amino acids in passion fruit were methionine and cystine. The 5 passion fruits were classified into 2 categories by using cluster analysis based on the TAA,EAA and delicious amino acid etc. ‘Guava Taste Golden Fruit’was classified as one category in view of containing of the highest amounts of TAA,EAA,CAA,FLA,BIA,MEA,BCA and ArAA. ‘Tai Nong’,‘Zi Xiang’,‘Man Tian Xing’and ‘Shi Sheng Zhu Xi Zi Guo’were classified as the other one category.