Abstract: The amino acid composition and nutritional qualities of 15 kinds of quinoa seeds from Qinghai,Yunnan,Peru,Hebei,Bolivia and other regions were evaluated by amino acid automatic analyzer. The results showed that the total amino acid content of quinoa seeds from different regions were 9.81% to 12.68%,the range of EAA/TAA were 34.5% to 36.0%,and the range of EAA/NEAA were 52.0% to 56.0%. Among them,Guyuan County white quinoa seed(Q13)EAA/TAA was 36.0%,EAA/NEAA was 56.0%,and its quality was the best,which could be used as an ideal resource for the development and utilization of quinoa seed protein products. The average amino acid ratio coefficient(SRC)of quinoa seeds was 88.9,and the amino acid ratio coefficient(RC)of sulfur-containing amino acids(methionine,cysteine)was the smallest,which was the first limiting amino acid. It can be consumed with foods with high levels of methionine and cystine to improve the edible value.