基于高效液相色谱法测定水产品中甘氨酸甜菜碱

丁源; 邢家溧; 承海; 郑睿行; 徐晓蓉; 丁超琼; 毛玲燕; 沈坚; 严小军; 李和生

doi:10.13386/j.issn1002-0306.2019.15.030

基于高效液相色谱法测定水产品中甘氨酸甜菜碱

Determination of Glycine Betaine in Aquatic Products by High Performance Liquid Chromatography

摘要

摘要: 应用高效液相色谱技术,建立水产品中甘氨酸甜菜碱定量检测方法。针对甘氨酸甜菜碱极性特点,选用BEH HILIC色谱柱,以乙腈:水为80:20构成优化的流动相,等度洗脱,紫外法于195 nm波长处检测甘氨酸甜菜碱特征峰。结果表明在15~200 μg/mL浓度范围内,检测目标物浓度与特征峰面积呈良好线性关系(R²=0.9999),方法检出限为5.12 μg/mL,定量限为12.00 μg/mL,平均回收率在96.5%~97.7%,相对标准偏差(RSD)为1.0%~2.2%。三类水产品实样测定,梭子蟹、对虾、花蛤所含甘氨酸甜菜碱分别为0.435、1.685和4.384 mg/g,相对标准偏差分别为0.5%、0.5%及1.8%。本方法简单快速、灵敏度高、重复性好,适用于水产品中甘氨酸甜菜碱定量检测。

Abstract: A method for quantitative determination of glycine betaine in aquatic products was developed by high performance liquid chromatography. In view of the hydrophilicity of glycine betaine,the BEH HILIC chromatographic column was selected for analysis,the optimized mobile phase was formed by using acetonitrile with water at 80:20,which was used for equal elution,and the content of glycine betaine was analyzed at the wavelength of 195 nm. The results showed that the method had good linearity in the range of 15~200 μg/mL concentration (R²=0.9999). The detection limit was 5.12 μg/mL,the quantitative limit was 12.00 μg/mL,the average recovery was 96.5%~97.7%,the relative standard deviation (RSD) was 1.0%~2.2%. The content of glycine betaine in swimming crab,prawn and clam was 0.435,1.685 and 4.384 mg/g,respectively. The relative standard deviation was 0.5%,0.5% and 1.8%,respectively. This method is simple,rapid,sensitive and reproducible. It is suitable for the quantitative detection of glycine betaine in aquatic products.

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