Abstract: In this paper,the β-glucosidase in peel and pulp of Jincheng oranges was extracted and purified. The results showed that the activity of β-glucosidase in 0.35 mol/L NaCl eluent from peel was (6.57±4.27)×10^-3 U/g,which was significantly higher than that in 0.4 mol/L NaCl eluent,and it was also higher than that in 0.35 mol/L and 0.4 mol/L NaCl eluent from pulp (p<0.05). The protein content in 0.35 mol/L NaCl eluent from peel was also the highest at the level of 0.154 mg/mL. The molecular weight of this β-glucosidase was higher than 170 kDa,and the maximum fluorescence absorption was at 582 nm,and the size was about 5~25 nm.