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中国精品科技期刊2020
牛婧娥, 吴国泰, 杜丽东, 李倩, 史彦斌, 任远. 当归润肠膏的制备工艺和质量标准[J]. 食品工业科技, 2019, 40(13): 107-112,140. DOI: 10.13386/j.issn1002-0306.2019.13.018
引用本文: 牛婧娥, 吴国泰, 杜丽东, 李倩, 史彦斌, 任远. 当归润肠膏的制备工艺和质量标准[J]. 食品工业科技, 2019, 40(13): 107-112,140. DOI: 10.13386/j.issn1002-0306.2019.13.018
NIU Jing-e, WU Guo-tai, DU Li-dong, LI Qian, SHI Yan-bin, REN Yuan. Preparation Technology and Quality Standard of DangguiRunchang Paste[J]. Science and Technology of Food Industry, 2019, 40(13): 107-112,140. DOI: 10.13386/j.issn1002-0306.2019.13.018
Citation: NIU Jing-e, WU Guo-tai, DU Li-dong, LI Qian, SHI Yan-bin, REN Yuan. Preparation Technology and Quality Standard of DangguiRunchang Paste[J]. Science and Technology of Food Industry, 2019, 40(13): 107-112,140. DOI: 10.13386/j.issn1002-0306.2019.13.018

当归润肠膏的制备工艺和质量标准

Preparation Technology and Quality Standard of DangguiRunchang Paste

  • 摘要: 本文对当归润肠膏的制备工艺和质量标准进行了研究,并考察其稳定性,为贮藏条件和保质期制定提供理论参考。采用正交试验L9(34)优选最佳提取工艺;用薄层层析和高效液相色谱法对当归润肠膏的主要成分进行了定性鉴别和定量分析;采用单因素实验及经典恒温加速试验考察稳定性,预测其有效期。当归润肠膏的最佳提取工艺:加入10倍处方量药材的水(w/v)浸泡1.25 h,回流提取2 h,趁热过滤;滤渣中再加8倍水量(w/v),再回流提取1.5 h;合并滤液。按此操作平行制备3批样品,其出膏得率、浸出物含量分别为64.24%±0.99%、21.43%±0.68%,多糖、阿魏酸含量分别为(7.78±0.03)、(4.05±0.24) mg/mL。将滤液浓缩至相对密度为1.207±0.008(70 ℃)的清膏,加入1倍清膏量的炼蜜(w/w)得到煎膏剂。在薄层层析谱图中可检出当归、大枣、干姜和枳壳的特征斑点,煎膏剂中阿魏酸的含量为(102.7±4.4) μg/g。经强光照射(20 ℃、4500 lx)和高温储存(避光、60 ℃)10 d后,阿魏酸的含量均显著降低。未添加稳定剂的当归润肠膏预测保质期约3个月,加入0.4%的NaHSO3、0.1%的EDTA-2Na作为稳定剂后,其预测保质期延长至1.68年,比未添加稳定剂的样品保质期延长了8倍。当归润肠膏的制备方法简单可行,质量相对稳定可控。

     

    Abstract: In this article, the preparation process and quality standards of DangguiRunchang paste (DRP) were studied, and its stability was also investigated to provide theoretical reference for storage conditions and shelf life. Orthogonal test L9 (34) was used to optimize the extraction process. The main components of DRP were qualitatively identified and quantitatively analyzed by Thin-layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC) method. The single factor test and the classical constant temperature accelerated test were used to investigate the stability and predict the shelf life. The optimal extraction conditions were:raw herbal materials were soaked with 10 times the amount of water (w/v) for 1.25 h, refluxed for 2 h, filtered while the extract was hot. The solid residue was extracted with 8 times the amount of water (w/v) again for 1.5 h. According to the above-mentioned method, 3 batches of samples were prepared in parallel and the yield and extractive content of the extracts were 64.24%±0.99% and 21.43%±0.68%, respectively. The polysaccharide and ferulic acid contents were (7.78±0.03) and (4.05±0.24) mg/mL, respectively. The filtrates were combined and concentrated to the relative density of 1.207±0.008 (70℃). The equal amount of refined honey (w/w) was added, mixed well to obtain the final preparation. The marker components of Radix Angelicae Sinensis, Zingibeais Rhizoma, Aurantii Frucrus and Jujubae Fructus were identified using TLC analyses. The content of ferulic acid in the DRP was (102.7±4.4) μg/g. After 10 days of exposure to strong light (20℃, 4500 xl) and high temperature storage (protection from light, 60℃), the content of ferulic acid significantly reduced. The DRP without stabilizers had a shelf life about 3 mouths. After added 0.4% NaHSO3 and 0.1% EDTA-2Na as stabilizers, its shelf life was extended to about 1.68 years, which was 8 times longer than that of the sample without stabilizers. The preparation technology of DRP was simple and feasible. The quality was relatively stable and controllable.

     

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