Abstract: To increase Cordyceps militaris yield and utilization efficiency of culture medium,10 groups of multigrain culture medium for Cordyceps militaris were made up,the growth rates and productivity of fruit bodies,and sensory evaluation of coarser grains porridge made of culture medium were compared. Then,3 superior multigrain culture medium were selected out for further analyzing their active substance content of fruit bodies,and the amino acids and flavor composition of culture medium. Results showed that the selected three groups were oats medium(oats 25 g),complex medium 6(oats 5 g+brown rice 5 g+soybean 5 g+lotus seed 10 g)and brown rice medium(brown rice 25 g). Among them,the Cordyceps militaris fruiting bodies cultured by oat medium and complex medium 6 showed fast growth rate and high production performance,and their sensory evaluation of coarser grains porridge were better. The sensory evaluation of multigrain porridge made of brown rice medium was the highest. The two amino acids with the highest content in the three groups were Pro and Glu. The maximum TAV was glutamate(33.65~46.95). The medium with the highest organic acid content was oat medium and the lowest was brown rice medium. The TAV value of malic acid and succinic acid in oats medium were 12.49 and 19.65,respectively. The TAV value of tartaric acid in brown rice medium was 7.27,and which of succinic acid inmedium 6 was 30.09,those made great contribution in tastes. Results showed that the culture of cordyceps militaris with suitable multigrain medium provided high fruiting body yield,and better taste of Cordyceps militaris multigrain porridge,which provided a theoretical basis for improving the exploitation and utilization level of the medium.