Abstract: The insulin resistant models of HepG2 cells were induced by insulin to study the effects of D-pinitol compound with Mn²⁺ on glucose consumption and glycogen synthesis. The expression of AMPK genes was detected with real-time polymerase chain reaction(RT-PCR). The results showed that when the insulin concentration was 1×10^-3 mmol/L and the action time was 36 h,the glucose consumption reached the minimum. Compared with the model group,when the D-pinitol concentration was 100 mg/L and MnSO₄ was 10 mg/L,glucose consumption and glycogen content were extremely significantly(p<0.01). The mRNA expression levels of AMPKα-1,AMPKα-2 and GLUT4 increased significantly(p<0.05)while G6pase mRNA expression decreased significantly(p<0.05). D-pinitol compound with Mn²⁺ could improve the glucose consumption,increase glycogen synthesis. Moreover,it had a positive effect on hypoglycemic activity through AMPK signaling pathway.