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中国精品科技期刊2020
张杨波, 兰时乐, 董新荣, 黄建安, 刘仲华. 紫甘薯α-淀粉酶酶解液中紫色素的分离与糖液发酵生产乙醇的研究[J]. 食品工业科技, 2019, 40(9): 184-188. DOI: 10.13386/j.issn1002-0306.2019.09.032
引用本文: 张杨波, 兰时乐, 董新荣, 黄建安, 刘仲华. 紫甘薯α-淀粉酶酶解液中紫色素的分离与糖液发酵生产乙醇的研究[J]. 食品工业科技, 2019, 40(9): 184-188. DOI: 10.13386/j.issn1002-0306.2019.09.032
ZHANG Yang-bo, LAN Shi-le, DONG Xin-rong, HUANG Jian-an, LIU Zhong-hua. Study on Separation of Purple Pigment from α-Amylase Enzymatic Hydrolyzate of Purple Sweet Potato and Production of Ethanol with the Sugar Liquid by Fermentation[J]. Science and Technology of Food Industry, 2019, 40(9): 184-188. DOI: 10.13386/j.issn1002-0306.2019.09.032
Citation: ZHANG Yang-bo, LAN Shi-le, DONG Xin-rong, HUANG Jian-an, LIU Zhong-hua. Study on Separation of Purple Pigment from α-Amylase Enzymatic Hydrolyzate of Purple Sweet Potato and Production of Ethanol with the Sugar Liquid by Fermentation[J]. Science and Technology of Food Industry, 2019, 40(9): 184-188. DOI: 10.13386/j.issn1002-0306.2019.09.032

紫甘薯α-淀粉酶酶解液中紫色素的分离与糖液发酵生产乙醇的研究

Study on Separation of Purple Pigment from α-Amylase Enzymatic Hydrolyzate of Purple Sweet Potato and Production of Ethanol with the Sugar Liquid by Fermentation

  • 摘要: 本文综合利用紫甘薯资源,同时获得紫色素和糖液,糖液发酵制备乙醇。以大孔树脂分离紫甘薯α-淀粉酶酶解液后获得紫色素与糖液,考察氮源种类与添加量、酵母接种量、发酵时间对糖液发酵产乙醇含量的影响,通过正交实验优化糖液发酵生产乙醇工艺。实验结果表明,紫甘薯酶解液经HPD400型大孔树脂分离,紫色素的色价(E1 cm1%)达到86.3(波长为530 nm),糖液中总糖含量达到55.5 mg/L;(NH4)2SO4作为发酵氮源,(NH4)2SO4添加量为3.0 g/100 mL、酵母接种量为1.5 mL、发酵时间为7 d,可得到13.0%vol乙醇。此方法获得了紫甘薯色素,副产物糖液发酵可生产乙醇,为紫甘薯的综合利用提供了思路。

     

    Abstract: In this paper,purple sweet potato resources were comprehensively utilized,purple pigment and sugar liquid were extracted at the same time,and sugar liquid was further fermented to prepare ethanol. The purple pigment and sugar liquid from purple sweet potato α-amylase hydrolysate separated by macroporous resin,the effects of kinds and amounts of nitrogen source,yeast dosage and fermentation time on producing ethanol by fermentation of sugar liquid were investigated and optimized the process of ethanol production by sugar fermentation through orthogonal experiment. The results showed that the purple sweet potato enzymatic hydrolysate was separated by HPD400 macroporous resin. The color value of purple pigment was E1 cm1%=86.3(at 530 nm)and the content of total sugar in solution was 55.5 mg/L. (NH4)2SO4 as nitrogen source with a dosage of 3.0 g in 100 mL sugar solution,the yeast of 1.5 mL and the fermentation time of 7 day. Under the conditions,an ethanol solution with 13.0%vol concentration was obtained. In the process,purple sweet potato pigment was obtained and ethanol was also got by fermentation of the by-product sugar solution,which provides a reference for comprehensive utilization of purple sweet potato.

     

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